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Measurement of plasma and urine carnitine in patients with cardiomyopathy, renal failure and metabolic abnormalities.January 1994 (has links)
by Leung Cheuk Wa. / Thesis (M.Sc.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 97-106). / ACKNOWLEDGEMENTS --- p.i / LIST OF FIGURES --- p.v / LIST OF TABLES --- p.vi / SUMMARY --- p.1 / Chapter 1. --- INTRODUCTION --- p.3 / Chapter 2. --- BASIC ASPECTS OF CARNITINE / Chapter 2.1 --- BIOSYNTHESIS OF CARNITINE --- p.5 / Chapter 2.2 --- CARNITINE TRANSPORT --- p.8 / Chapter 2.3 --- THE ROLE OF CARNITINE IN INTRACELLULAR METABOLISM --- p.10 / Chapter 2.4 --- THE ROLE OF KIDNEY IN CARNITINE METABOLISM --- p.16 / Chapter 3. --- CARNITINE DEFICIENCY --- p.18 / Chapter 3.1 --- PRIMARY CARNITINE DEFICIENCY --- p.20 / Chapter 3.1.1 --- MYOPATHIC CARNITINE DEFICIENCY --- p.21 / Chapter 3.1.2 --- SYSTEMIC CARNITINE DEFICIENCY --- p.21 / Chapter 3.2 --- SECONDARY CARNITINE DEFICIENCY --- p.22 / Chapter 4. --- CARNITINE METABOLISM IN SELECTED DISEASES / Chapter 4.1 --- CARDIOMYOPATHY --- p.23 / Chapter 4.2 --- ORGANIC ACIDURIAS --- p.24 / Chapter 4.3 --- VALPROIC ACID THERAPY --- p.26 / Chapter 4.4 --- RENAL DIALYSIS ANDTRANSPLANTATION --- p.28 / Chapter 5. --- ANALYTICAL METHODS FOR CARNITINE ASSAYS --- p.30 / Chapter 6. --- DETERMINATION OF TOTAL AND FREE CARNITINE / Chapter 6.1 --- PRINCIPLE OF THE ASSAYS --- p.32 / Chapter 6.1.1 --- FREE CARNITINE DETERMINATION --- p.32 / Chapter 6.1.2 --- TOTAL CARNITINE DETERMINATION --- p.33 / Chapter 6.2 --- INSTRUMENTATION --- p.34 / Chapter 6.3 --- PREPARATION OF REAGENTS AND STANDARDS --- p.36 / Chapter 6.4 --- SPECIMEN COLLECTION --- p.42 / Chapter 6.5 --- SAMPLE PREPARATION --- p.43 / Chapter 6.6 --- ASSAY PROTOCOL FOR FREE CARNITINE --- p.44 / Chapter 6.7 --- ASSAY PROTOCOL FOR TOTAL CARNITINE --- p.46 / Chapter 6.8 --- FACTORS AFFECTING THE PERFORMANCE OF ASSAYS --- p.48 / Chapter 6.9 --- EVALUATION OF FREE AND TOTAL CARNITINE ASSAYS --- p.50 / Chapter 7. --- RESULTS OF EVALUATION OF TOTAL AND FREE CARNITINE ASSAYS / Chapter 7.1 --- CALIBRATION --- p.52 / Chapter 7.2 --- PRECISION --- p.55 / Chapter 7.3 --- LINEARITY RANGE --- p.56 / Chapter 7.4 --- RECOVERY --- p.58 / Chapter 7.5 --- INTERFERENCE OF ACETYLCARNITINE ON FREE CARNITINE ASSAY --- p.59 / Chapter 7.6 --- DISCUSSION --- p.59 / Chapter 8. --- STUDY IN NORMAL SUBJECTS / Chapter 8.1 --- SUBJECTS --- p.61 / Chapter 8.2 --- RESULTS OF THE NORMAL SUBJECTS --- p.61 / Chapter 8.3 --- DISCUSSION --- p.63 / Chapter 9. --- PATIENTS STUDY / Chapter 9.1 --- PATIENTS WITH CARDIOMYOPATHY / Chapter 9.1.1 --- SUBJECTS --- p.66 / Chapter 9.1.2 --- RESULTS OF THE STUDY --- p.66 / Chapter 9.1.3 --- DISCUSSION --- p.69 / Chapter 9.2 --- PATIENTS WITH METABOLIC DISEASES / Chapter 9.2.1 --- SUBJECTS --- p.71 / Chapter 9.2.2 --- RESULTS OF THE STUDY --- p.71 / Chapter 9.2.3 --- DISCUSSION --- p.74 / Chapter 9.3 --- PATIENTS ON VALPROIC ACID THERAPY / Chapter 9.3.1 --- SUBJECTS --- p.75 / Chapter 9.3.2 --- RESULTS OF THE STUDY --- p.75 / Chapter 9.3.3 --- DISCUSSION --- p.77 / Chapter 9.4 --- PATIENTS ON RENAL DIALYSIS AND AFTER TRANSPLANTATION / Chapter 9.4.1 --- SUBJECTS --- p.79 / Chapter 9.4.2 --- RESULTS OF THE STUDY --- p.79 / Chapter 9.4.3 --- DISCUSSION --- p.81 / Chapter 10. --- GENERAL DISCUSSION --- p.84 / Chapter 11. --- REFERENCES --- p.97
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Serum high-sensitivity C-reactive protein concentration of Chinese chronic-renal-failure patients with atherosclerotic vascular disease or cardiac valve calcification.January 2002 (has links)
Chan Fat-Yiu. / Thesis (M.Sc.)--Chinese University of Hong Kong, 2002. / Includes bibliographical references (leaves 85-93). / Abstracts in English and Chinese. / ACKNOWLEDGEMENTS --- p.4 / SUMMARY --- p.5 / ABBREVIATIONS --- p.9 / LIST OF TABLES --- p.11 / LIST OF FIGURES --- p.13 / Chapter CHAPTER I --- INTRODUCTION --- p.14 / Chapter 1.1 --- The Historical Aspects of C-Reaction Protein --- p.15 / Chapter 1.2 --- Biochemistry of CRP --- p.16 / Chapter 1.3 --- Physiology of CRP --- p.18 / Chapter 1.4 --- Current Clinical Applications of Serum CRP Assay --- p.19 / Chapter 1.5 --- Recent Findings of CRP --- p.21 / Chapter 1.5.1 --- Pathophysiology of atherosclerosis --- p.22 / Chapter 1.5.2 --- A nother atherogenic risk factor: hs- CRP --- p.26 / Chapter 1.5.3 --- Can hs-CRP replace other risk factors? --- p.30 / Chapter 1.5.4 --- Altering hs-CRP result in medication --- p.32 / Chapter 1.6 --- Methods of Measurement of CRP Concentration --- p.33 / Chapter 1.7 --- Analytical Considerations in the Measurement of hs-CRP --- p.34 / Chapter CHAPTER II --- OBJECTIVES AND SIGNIFICANCE --- p.36 / Chapter 2.1 --- Objectives --- p.37 / Chapter 2.2 --- Issues and Problems --- p.37 / Chapter 2.3 --- Significance and Value of this Study --- p.38 / Chapter CHAPTER III --- MA TERIALS AND METHODS I Setting up the serum hs-CRP assay on the Hitachi 911 Analyzer --- p.39 / Chapter 3.1 --- Materials --- p.40 / Chapter 3.1.1 --- Reagents from Roche Diagnostics --- p.40 / Chapter 3.1.2 --- Reagents for the Beckman Coulter Array ® Analyzer --- p.40 / Chapter 3.1.3 --- In-house reagents --- p.41 / Chapter 3.2. --- Apparatus and Equipment --- p.41 / Chapter 3.2.1 --- Hitachi 911 Analyzer --- p.41 / Chapter 3.2.2 --- Beckman Coulter Array ® 360 Analyzer --- p.42 / Chapter 3.3 --- The Tina-quant a C-Reactive Protein (Latex) Ultrasensitive Assay --- p.42 / Chapter 3.3.1 --- Priniciple of the Dual-Radius Enhanced Latex (DuREL´ёØ) technology --- p.42 / Chapter 3.3.2 --- Assessment of Analytical Performance --- p.45 / Chapter CHAPTER IV --- MA TERIALS AND METHODS II Serum hs-CRP in Chinese chronic-renal-failure patients with atherosclerotic vascular disease or cardiac valve calcification --- p.48 / Chapter 4.1 --- Patient Recruitment --- p.49 / Chapter 4.2. --- Blood Specimens --- p.49 / Chapter 4.3 --- Assay Methods --- p.50 / Chapter 4.3.1 --- hs-CRP --- p.50 / Chapter 4.3.2 --- TC --- p.50 / Chapter 4.3.3 --- TG --- p.51 / Chapter 4.3.4 --- HDL-C --- p.51 / Chapter 4.3.5 --- LDL-C --- p.52 / Chapter 4.3.6 --- Apo A-1 --- p.52 / Chapter 4.3.7 --- Apo B --- p.53 / Chapter 4.3.8 --- Lp(a) --- p.53 / Chapter 4.4 --- Ultrasound measurement of carotid artery inter-media thickness --- p.53 / Chapter 4.5 --- Statistical analysis --- p.54 / Chapter CHAPTER V --- RESUTLSI Setting up the serum hs-CRP assay on the Hitachi 911 Analyzer --- p.55 / Chapter 5.1 --- Imprecision --- p.56 / Chapter 5.2 --- Linearity --- p.56 / Chapter 5.3 --- Recovery --- p.56 / Chapter 5.4 --- Detection Limit --- p.57 / Chapter 5.5 --- Carry-over --- p.57 / Chapter CHAPTER VI --- RESULTS II Serum hs-CRP in Chinese chronic-renal-failure patients with atherosclerotic vascular disease or cardiac valve calcification --- p.63 / Chapter 6.1 --- Patient Recruitment --- p.64 / Chapter 6.2 --- Chinese chronic-renal-failure patients with AVD --- p.64 / Chapter 6.3 --- Chinese chronic-renal-failure patients with CVC --- p.65 / Chapter CHAPTER VII --- DISCUSSION I Performance of the serum hs-CRP assay on the Hitachi 911 Analyzer --- p.75 / Chapter 7.1 --- "Imprecision, Detection Limit, Linearity, and Recovery of hs-CRP Assay" --- p.76 / Chapter 7.1.1 --- Imprecision --- p.76 / Chapter 7.1.2 --- Detection Limit --- p.76 / Chapter 7.1.3 --- Linearity --- p.76 / Chapter 7.1.4 --- Recovery --- p.77 / Chapter 7.2 --- Overall Performance --- p.77 / Chapter CHAPTER VIII --- DISCUSSION II Serum hs-CRP in Chinese chronic-renal-failure patients with atherosclerotic vascular disease or cardiac valve calcification --- p.79 / Chapter 8.1 --- CAPD Patients --- p.80 / Chapter 8.2 --- Serum hs-CRP Concentration of AVD and CVC Patients --- p.81 / Chapter 8.3 --- Other risk factors in AVD and CVC Patients --- p.82 / Chapter 8.4 --- Conclusion --- p.83 / REFERENCES --- p.85
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