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The aim of the thesis is a realization of advanced analysis of signals from gait laboratory. The introductory part deals with the gait cycle and its relation to the joints kinematic is discussed. Additionally, the work is focused on the description of the gait laboratory and the definition of the indexes in order to quantify patient´s overall gait in kinematic analysis. In the practical part, kinematic data analysis was implemented in the MATLAB environment and the results of healthy individuals and patients with cerebral palsy were compared. Kinematic analysis included peak detection in specific kinematic variables. In the last part a graphical user interface for visualization was implemented.