This dissertation thesis composes of two parts; the first part focus on the characterization of trypsin, enzyme frequently used in proteomic research for the investigation and identification of protein sequences, and its peptide digestion kinetics. The second part is aimed to the enantioseparations of biologically active compounds. First part of this project focus on tryptic digestion of synthetic peptides and the development of HPLC method for the identification of synthetic peptides and their fragments. Using the in-solution digestion and HPLC method, relative kinetic constants were determined for problematic sequences. Amino acids responsible for the decrease in trypsin catalytic activity and their location towards the cleavage site were studied. Certain slight exopeptidase activity of trypsin was noted, especially at the end of peptide chain. Furthermore, three columns with immobilized trypsin used in HPLC were compared concerning their catalytic activity. The immobilization of enzymes on solid support is used to elevate the amount of enzyme present during digestion and to assure better repeatability and reproducibility of obtained results. Activity of a new trypsin column synthesized at the University of North Carolina at Chapel Hill was compared to two commercially available trypsin columns....

IN ENGLISH Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Supervisors: prof. Dr. Michael Gütschow RNDr. Klára Konečná, Ph.D. Candidate: Michaela Hympánová Title of the diploma thesis: Enzyme kinetic evaluation of several potential inhibitors of certain human cysteine and serine proteases Background Cysteine and serine proteases are enzymes involved in many physiological processes. The imbalance between them and their endogenous inhibitors is associated with various diseases such as cancer and osteoporosis. Synthetic inactivators could be useful in the treatment of these enzyme-mediated pathological conditions. Therefore, there are ongoing attempts to develop low-molecular weight inactivators for therapeutically relevant cysteine and serine proteases. In the course of this thesis, compounds synthesized in prof. Gütschow's group were investigated as potential inhibitors of selected human proteases. They belong to imidazole compounds derived from N-protected cyclohexylalanine, 2-phenyl-7,8-dihydroimidazo[1,2- a]pyrazin-6(5H)-one derivatives, ,-unsaturated peptidomimetic compounds, carbamates, an N,N-dibenzylcrotonamide derivatives and peptoides. Aims This diploma thesis has been focused on the evaluation of new potential inhibitors against...

The inhibition of HIV-1 protease plays an important role in combating HIV. Nine HIV-1 protease inhibitors have been succesfully marketed for the treatment since 1995. However, their efficiencies decrease due to the resistance development. More potent compounds with novel structural motifs and mechanisms of action are therefore still needed. Several inhibitory compounds have been reported to bind to the protease at the loci different from the active site. Interestingly, darunavir, which is the last approved inhibitor with supposedly competitive mode of action, was also suggested to bind to the flap region of the protease. Two studies discussed this alternative binding mode based on the X-ray structural and kinetic analysis, respectively. Nevertheless, it is questionable, if such a mechanism is relevant also in physiological conditions or if it is only an artifact of crystallization. Another study provided a strong evidence for the alternative binding of darunavir to highly mutated HIV-1 protease. Based on thermodynamic analysis, it was shown that two molecules of darunavir bind to the protease dimer. Surprisingly, this observation was not confirmed by the X-ray structure analysis since the inhibitor was bound only within the active site. However, this protease variant was employed in further...

The world energy supply and demand, together with limited available resources have resulted in the need to develop alternative energy sources to ensure sustainable and expanding economies. Hydrogen is being considered a viable option with particular application to fuel cells. The Hybrid Sulphur cycle has been identified as a process to produce clean hydrogen (carbon free process) and can have economic benefits when coupled to nuclear reactors (High Temperature Gas Reactor) or solar heaters for the supply of the required process energy. The sulphur trioxide decomposition reactor producing sulphur dioxide for the electrolytic cells in a closed loop system has been examined, but it is clear that development with respect to a more durable active catalyst in a reactor operating under severe conditions needs to be investigated. A suitable sulphur trioxide reactor needs to operate at a high temperature with efficient heating in view of the endothermic reaction, and has to consist of special materials of construction to handle the very corrosive reactants and products. This investigation was undertaken to address (1) the synthesis, characterisation, reactivity and stability of a suitable catalyst (2), determination the reaction rate of the chosen catalyst with a suitable micro reactor (3) construction and evaluation of a packed bed reactor for the required reaction, and (4) the development and validation of a reactor model using computational fluid dynamics with associated chemical reactions. A supported catalyst consisting of 0.5 wt% platinum and 0.5 wt% palladium on rutile (TiO2, titania) was prepared by the sintering of an anatase/rutile supported catalyst with the same noble metal composition, synthesized according to an incipient impregnation procedure using cylindrical porous pellets (±1.7 mm diameter and ±5 mm long). Characterization involving: surface area, porosity, metal composition, - dispersion, - particle size, support phase and sulphur content was carried out and it was found from reactivity determinations that the sintered catalyst, which was very different from the synthesized catalyst, had an acceptable activity and stability which was suitable for further evaluation. A micro pellet reactor was constructed and operated and consisted of a small number of pellets (five) placed apart from each other in a two-stage quartz reactor with sulphur trioxide generated from sulphuric acid in the first stage and the conversion of sulphur trioxide in the second stage, respectively. Attention was only confined to the second stage involving the conversion of sulphur trioxide with the supported catalyst. The overall reaction kinetics of the pellets involving momentum, heat and mass transfer and chemical reaction was evaluated and validated with constants obtained from literature and with an unknown reaction rate equation for which constants were obtained by regression. As result of the complexity of the flow, mass and heat transfer fields in the micro pellet reactor it was necessary to use a CFD model with chemical reactions which was accomplished with a commercial code COMSOL MultiPhysics® 4.3b. A reversible reaction rate equation was used and a least squares regression procedure was used to evaluate the activation energy and pre-exponential factor. The activation energy obtained for the first order forward reaction was higher than values obtained from literature for a first order reaction rate (irreversible reaction) for the platinum group metals on titania catalysts. Detailed analyses of the velocity, temperature and concentration profile revealed the importance of using a complex model for determination of the reaction parameters. A fixed bed reactor system consisting of a sulphuric acid vaporizer, a single reactor tube (1 m length, 25 mm OD) heated with a surrounding electrical furnace followed, by a series of condensers for the analysis of the products was constructed and operated. Three process variables were investigated, which included the inlet temperature, the weight hourly velocity and the residence time in order to assess the performance of the reactor and generate results for developing a model. The results obtained included the wall and reactor centreline temperature profiles together with average conversion. As a result of the complexity of the chemistry and the phases present containing the products from the reactor a detailed calculation was done using vapour/liquid equilibrium with the accompanying mass balance (Aspen-Plus®) to determine the distribution of sulphur trioxide, sulphur dioxide, oxygen and steam. A mass balance was successfully completed with analyses including SO2 with a GC, O2 with a paramagnetic cell analyser, acid/base titrations with sodium hydroxide, SO2 titrations with iodine and measurement of condensables (mass and volume). The results obtained showed that a steady state (constant conversion) was obtained after approximately six hours and that it was possible to obtain sulphur trioxide conversion approaching equilibrium conditions for bed lengths of 100 mm with very low weight hourly space velocities. A heterogeneous 2D model consisting of the relevant continuity, momentum, heat transfer and mass transfer and the reaction rate equation determined in this investigation was developed and solved with the use of the commercial code COMSOL MultiPhysics® 4.3b with an appropriate mesh structure. The geometry of the packed bed (geometry) was accomplished by generating a randomly packed bed with a commercial package DigiPac™. The model predicted results that agreed with experimental results with conversions up to 56%, obtained over the following ranges: weight hourly space velocity equal to 15 h-1, temperatures between 903 K and 1053 K and residence times between 0.1 and 0.07 seconds. The post-processing results were most useful for assessing the effect of the controlling mechanisms and associated parameters. / PhD (Chemical Engineering), North-West University, Potchefstroom Campus, 2014

The world energy supply and demand, together with limited available resources have resulted in the need to develop alternative energy sources to ensure sustainable and expanding economies. Hydrogen is being considered a viable option with particular application to fuel cells. The Hybrid Sulphur cycle has been identified as a process to produce clean hydrogen (carbon free process) and can have economic benefits when coupled to nuclear reactors (High Temperature Gas Reactor) or solar heaters for the supply of the required process energy. The sulphur trioxide decomposition reactor producing sulphur dioxide for the electrolytic cells in a closed loop system has been examined, but it is clear that development with respect to a more durable active catalyst in a reactor operating under severe conditions needs to be investigated. A suitable sulphur trioxide reactor needs to operate at a high temperature with efficient heating in view of the endothermic reaction, and has to consist of special materials of construction to handle the very corrosive reactants and products. This investigation was undertaken to address (1) the synthesis, characterisation, reactivity and stability of a suitable catalyst (2), determination the reaction rate of the chosen catalyst with a suitable micro reactor (3) construction and evaluation of a packed bed reactor for the required reaction, and (4) the development and validation of a reactor model using computational fluid dynamics with associated chemical reactions. A supported catalyst consisting of 0.5 wt% platinum and 0.5 wt% palladium on rutile (TiO2, titania) was prepared by the sintering of an anatase/rutile supported catalyst with the same noble metal composition, synthesized according to an incipient impregnation procedure using cylindrical porous pellets (±1.7 mm diameter and ±5 mm long). Characterization involving: surface area, porosity, metal composition, - dispersion, - particle size, support phase and sulphur content was carried out and it was found from reactivity determinations that the sintered catalyst, which was very different from the synthesized catalyst, had an acceptable activity and stability which was suitable for further evaluation. A micro pellet reactor was constructed and operated and consisted of a small number of pellets (five) placed apart from each other in a two-stage quartz reactor with sulphur trioxide generated from sulphuric acid in the first stage and the conversion of sulphur trioxide in the second stage, respectively. Attention was only confined to the second stage involving the conversion of sulphur trioxide with the supported catalyst. The overall reaction kinetics of the pellets involving momentum, heat and mass transfer and chemical reaction was evaluated and validated with constants obtained from literature and with an unknown reaction rate equation for which constants were obtained by regression. As result of the complexity of the flow, mass and heat transfer fields in the micro pellet reactor it was necessary to use a CFD model with chemical reactions which was accomplished with a commercial code COMSOL MultiPhysics® 4.3b. A reversible reaction rate equation was used and a least squares regression procedure was used to evaluate the activation energy and pre-exponential factor. The activation energy obtained for the first order forward reaction was higher than values obtained from literature for a first order reaction rate (irreversible reaction) for the platinum group metals on titania catalysts. Detailed analyses of the velocity, temperature and concentration profile revealed the importance of using a complex model for determination of the reaction parameters. A fixed bed reactor system consisting of a sulphuric acid vaporizer, a single reactor tube (1 m length, 25 mm OD) heated with a surrounding electrical furnace followed, by a series of condensers for the analysis of the products was constructed and operated. Three process variables were investigated, which included the inlet temperature, the weight hourly velocity and the residence time in order to assess the performance of the reactor and generate results for developing a model. The results obtained included the wall and reactor centreline temperature profiles together with average conversion. As a result of the complexity of the chemistry and the phases present containing the products from the reactor a detailed calculation was done using vapour/liquid equilibrium with the accompanying mass balance (Aspen-Plus®) to determine the distribution of sulphur trioxide, sulphur dioxide, oxygen and steam. A mass balance was successfully completed with analyses including SO2 with a GC, O2 with a paramagnetic cell analyser, acid/base titrations with sodium hydroxide, SO2 titrations with iodine and measurement of condensables (mass and volume). The results obtained showed that a steady state (constant conversion) was obtained after approximately six hours and that it was possible to obtain sulphur trioxide conversion approaching equilibrium conditions for bed lengths of 100 mm with very low weight hourly space velocities. A heterogeneous 2D model consisting of the relevant continuity, momentum, heat transfer and mass transfer and the reaction rate equation determined in this investigation was developed and solved with the use of the commercial code COMSOL MultiPhysics® 4.3b with an appropriate mesh structure. The geometry of the packed bed (geometry) was accomplished by generating a randomly packed bed with a commercial package DigiPac™. The model predicted results that agreed with experimental results with conversions up to 56%, obtained over the following ranges: weight hourly space velocity equal to 15 h-1, temperatures between 903 K and 1053 K and residence times between 0.1 and 0.07 seconds. The post-processing results were most useful for assessing the effect of the controlling mechanisms and associated parameters. / PhD (Chemical Engineering), North-West University, Potchefstroom Campus, 2014

This dissertation thesis focuses on creating tools for the analysis and potential therapeutic intervention in the biological processes regulated by proteolysis. I focus on two important proteolytic enzymes: HIV-1 protease, which is indispensable for the polyprotein processing of the nascent virus and thus for the development of infectious viral particle, and glutamate carboxypeptidase II, a tumor marker and a neuropeptidase from the prostate and central nervous system. Rational design of inhibitors of these therapeutically relevant enzymes serves two purposes: firstly, protease inhibitors were shown to be powerful drugs (HIV protease is in fact the example of successful drug development driven by structural biology). Secondly, and in the context of this thesis perhaps more importantly, inhibitors of medicinally relevant proteases might serve as tools for the elucidation of basic biological questions concerning regulation, timing and spatiotemporal control of such key processes as virus maturation or cancer development. The experimental work described in this thesis summarizes my results in both these areas. Human Immunodeficiency Virus Protease Human immunodeficiency virus (HIV), a causative agent of AIDS, has been estimated to kill close to 40 million people during the past four decades with 1.5...

<p>Su&scaron;enje dunje kao voćne vrste u praksi nije uobičajeno, zbog povi&scaron;ene vrijednosti<br />tvrdoće i čvrstoće. Prethodna istraživanja su&scaron;enja dunje, pokazala su da se osmotskim<br />su&scaron;enjem u okviru kombinovane tehnologije su&scaron;enja dobijaju povoljnije mehaničke<br />osobine su&scaron;enih proizvoda. Na osnovu plana obavljeni su eksperimenti osmotskog i<br />konvektivnog su&scaron;enja dunje u obliku četvrtina. Eksperiment osmotskog su&scaron;enja je<br />obavljen kao trofaktorni, gdje su uticajni faktori: temperatura rastvora, koncentracija<br />rastvora i vrijeme trajanja su&scaron;enja. TakoĎe, eksperiment konvektivnog su&scaron;enja je obavljen<br />kao trofaktorni, a uticajni faktori su: primjenjeni predtretman, temperature vazduha za<br />su&scaron;enje i brzina strujanja vazduha ispred sloja.<br />Na osnovu rezultata mjerenja eksperimenta osmotskog su&scaron;enja, zaključeno je da sa<br />porastom temperature i koncentracije rastvora postiže se veća brzina su&scaron;enja, &scaron;to se i<br />očekivalo. Disperzionom analizom osmotskog su&scaron;enja je ispitan uticaj faktora na brzinu<br />su&scaron;enja i zapreminsko skupljanje četvrtina dunje. Sa statističkom vjerovatnoćom od 99%<br />je dokazano da postoji uticaj svih faktora na brzinu su&scaron;enja uzoraka. Dokazano je sa<br />statističkom vjerovatnoćom od 99% da vrijeme trajanja osmotskog su&scaron;enja ima uticaj na<br />zapreminsko skupljanje četvrtina dunje, dok uticajni faktori temperatura i koncentracija<br />rastvora imaju uticaj sa statističkom vjerovatnoćom od 95%.<br />Analizom rezultata mjerenja veličina tokom eksperimenta konvektivnog su&scaron;enja<br />evidentno je da se sa vi&scaron;om temperaturom vazduha postiže brže sniženje vlažnosti<br />četvrtina dunje. Povećanjem vremena trajanja osmotskog predtretmana, smanjuje se<br />brzina konvektivnog su&scaron;enja. UtvrĎeno je da kori&scaron;ćenje osmotskog predtretmana sa<br />vi&scaron;om temperaturom rastvora i većom koncentracijom rastvora dovodi do smanjenja<br />brzine konvektivnog su&scaron;enja. Na osnovu disperzione analize konvektivnog su&scaron;enja sa<br />statističkom vjerovatnoćom od 99% dokazano je da postoje uticaji predtretmana i<br />temperature vazduha na brzinu su&scaron;enja i zapreminsko skupljanje četvrtina dunje. Uticaj<br />faktora brzine vazduha ispred sloja materijala nije dokazan disperzionom analizom, ali je<br />na osnovu kinetički krivih evidentan uticaj ovog faktora na brzinu su&scaron;enja, pri vi&scaron;im<br />vlažnostima materijala i vi&scaron;oj temperaturi konvektivnog su&scaron;enja. Najvjerovatniji razlog<br />ovakvih rezultata disperzione analize su male vrijednosti brzine vazduha za su&scaron;enje,<br />odnosno njihova mala razlika vrijednosti (0,5 m/s).<br />Kinetika prenosa mase tokom kombinovane tehnologije su&scaron;enja dunje u formi<br />četvrtine je matematički opisana, modelovanjem kinetike osmotskog su&scaron;enja i kinetike<br />konvektivnog su&scaron;enja. U okviru kinetike osmotskog su&scaron;enja četvrtina dunje, obavljeno je<br />matematičko modelovanje promjene vlažnosti u odnosu na suvu bazu i modelovanja<br />zapreminskog skupljanja. Za kinetiku konvektivnog su&scaron;enja četvrtina dunje, obavljeno je<br />matematičko modelovanje promjene vlažnosti u odnosu na suvu bazu tokom vremena<br />konvektivnog su&scaron;enja. Za svaku veličinu koja se modeluje usvojeno po tri matematička<br />modela, a modeli su pro&scaron;ireni uticajnim faktorima u bezdimenzionom obliku. Izvedeni<br />modeli su imali dobro slaganje sa eksperimentalnim rezultatima, &scaron;to pokazuju visoke<br />vrijednosti koeficijente korelacije. Primjena svih modela je jednostavna, gdje je uz pomoć<br />standardnih računara moguće brzo izračunavanje svih modelovanih veličina.<br />Primjenom osmotskog predtretmana u kombinovanoj tehnologiji su&scaron;enja dunje,<br />dobijaju se neke pozitivne osobine su&scaron;enog proizvoda. Smanjenje zapremine je manje<br />izraženo, nego primjenom samo konvektivnog su&scaron;enja. Vlažnost u voćnom tkivu je<br />ravnomjernije rasporeĎena i manje je presu&scaron;ivanje spoljnjih slojeva voćnog tkiva.<br />Stvaraju se manji unutra&scaron;nji naponi izmeĎu slojeva tkiva, uslijed čega nastaju manje<br />deformacije oblika. U povr&scaron;inskom sloju voćnog tkiva postoje adsorpcione veze vlage,<br />rastvorka i suve materije, a ove veze utiču na povećanje vrijednosti vlažnosti pri kojima<br />se osu&scaron;eni proizvod bezbjedno skladi&scaron;ti.</p> / <p>Drying of the quince as a fruit species is not common in practice due to its increased hardness<br />and firmness. Previous researches on quince drying have indicated that osmotic drying, within<br />the combined drying technology, enables favourable mechanical properties of dried products.<br />Planned experiments of the osmotic and convective drying of quince quarters were conducted.<br />The osmotic drying experiment was based on three significant experimental factors: the<br />temperature of osmotic solution, the concentration of osmotic solution, and the duration of<br />osmotic drying. The convective drying experiment was also based on three experimental<br />factors entailing the applied pretreatment, the temperature of drying air and the velocity of<br />drying air in front of material layers.<br />The measurement results obtained during the osmotic drying experiment indicate that an<br />increase in the temperature and the concentration of osmotic solution accelerates the drying<br />process, as expected. A dispersion analysis of the osmotic drying indicated the effects of<br />experimental factors on the drying speed and the volume shrinkage of quince quarters. With a<br />statistical probability of 99%, it was proven that all factors influence the speed of drying.<br />Moreover, with a statistical probability of 99%, it was proven that the duration of osmotic<br />drying influences the volume shrinkage of quince quarters, whereas the significant factors such<br />as the temperature and the concentration of osmotic solution exert influence with a statistical<br />probability of 95%.<br />The analysis of measurement results obtained during the convective drying experiment shows<br />that an increase in the air temperature accelerates a decrease in the moisture content of quince<br />quarters. An increase in the duration of osmotic pretreatment decelerates the convective drying<br />process. It was determined that the application of the osmotic pretreatment with higher<br />solution temperature and concentration decelerates the convective drying. On the basis of a<br />dispersion analysis of the convective drying with a statistical probability of 99%, it was proven<br />that the pretreatment and the air temperature affect the speed of drying and the volume<br />shrinkage of quince quarters. The effects of the air velocity in front of the material layer was<br />not determined by means of the dispersion analysis, nevertheless kinetic curves indicate the<br />influence of this factor on the drying speed, especially in the instance of higher moisture<br />content of the material and higher temperature of the convective drying. The most probable<br />causes of such dispersion analysis results are low values of the drying air velocity, i.e. a slight<br />difference in the values (0.5 m/s).<br />The kinetics of mass transfer during the combined drying of quince quarters was<br />mathematically described by means of the modelling of the kinetics of osmotic drying and the<br />kinetics of convective drying. The kinetics of osmotic drying of quince quarters was expressed<br />via the mathematical modelling of moisture content change in relation to dry basis and the<br />modelling of volume shrinkage. The kinetics of convective drying of quince quarters was<br />expressed via the mathematical modelling of moisture content change in relation to dry basis<br />during the convective drying. Three mathematical models were established for every modelled<br />value, and the models were extended by significant non-dimensional experimental factors. The<br />developed models had a good fit with the experimental results, which was indicated by the<br />high values of the correlation coefficient. The application of all models is simple and standard<br />personal computers can be used for rapid calculations of modelled values.<br />The application of the osmotic pretreatment in the combined quince drying enables<br />favourable properties of dried products. Volume shrinkage is slighter in comparison with the<br />convective quince drying. The moisture content in the fruit tissue is more evenly distributed<br />and the dehydration of the outer layers of the fruit tissue is lower. The tension between the<br />layers of the tissue is reduced thus reducing the shape defects. Within the surface layers of fruit<br />tissues, the adsorption bonds of moisture, solute and dry matter are formed. These bonds<br />increase the moisture content value, which enables the safe storage of dry products.</p>

Cytochromes P450 are the major xenobiotics converting enzymes. They are classified as mixed function monooxygenases (MFO). Isoform 1A1 is a extrahepatic form found mainly in the lung and other tissues. It is strongly induced by polycyclic aromatic hydrocarbons and their derivatives via the Ah receptor. As a marker reaction for this enzyme can be used hydroxylation of Sudan I, which has previously been widely used as a azo dye in industry, but since 1980s it is banned for coloring food and cosmetics for its negative influence on the organism. NADPH:cytochrome P450 reductase is the major electron donor for cytochrome P450 catalyzed monooxygenation reactions. Another electron carrier for cytochrome P450 catalyzed reactions is cytochrome b5. It was shown that cytochrome b5 can stimulate, inhibit or have no effect on P450 catalyzed reactions. This thesis aims to evaluate the influence of the ration between NADPH:cytochrome P450 reductase and cytochrome b5 on cytochrome P450 1A1 catalyzed Sudan I hydroxylation. The main goal is to characterize the influence of electron donor and electron transfer ratios on hydroxylation of Sudan I, and to determine the kinetic parameters KM and VMAX for selected protein ratios. Partial aims of the thesis were to characterize the recombinant proteins used in this study...

The thesis is devoted to the frequency-resolved investigation of the relaxation processes in optoelectronic materials and devices. Conventional fluorescence decay time measurement technique in the frequency domain was adapted for the use with light-emitting diode (LED) excitation and signal registration by a lock-in amplifier. Inorganic phosphors synthesized by aqueous sol-gel combustion method for using as wavelength converters in white LEDs as well as advanced organic semiconducting materials were investigated by photoluminescence decay time and quantum yield measurement techniques. The photoluminescence decay time measurement technique with extremely low quasi-continuous UV LED excitation was applied for the carrier dynamics research in GaN epitaxial layers. The investigation under such a low excitation conditions revealed the contribution of donor-acceptor recombination in the yellow luminescence of GaN. The techniques for in-situ thermal characterization of encapsulated LEDs, including the measurements of phosphors converter temperature and heat relaxation time constants inside a LED, were developed and demonstrated for the investigation of commercial low- and high- power LEDs. / Disertacija yra skirta relaksacijos procesų, vykstančių optoelektronikos medžiagose ir prietaisuose, tyrimui dažninės skyros metodu. Įprastas fluorescencijos gesimo trukmės tyrimo metodas, veikiantis harmoniškai moduliuoto žadinimo režimu, buvo adaptuotas žadinimui puslaidininkiniais šviestukais ir signalų registravimui radijo dažnių faziniu detektoriumi. Neorganiniai vandeniniu zolių-gelių metodu susintetinti fosforai, skirti baltų puslaidininkinių šviestukų gamybai, ir modernios organinės optoelektronikos medžiagos buvo ištirtos fotoliuminescencijos gesimo dėsnio ir kvantinės išeigos matavimo metodikomis, siekiant nustatyti krūvininkų rekombinacijos savybes ir optimizuoti sintezės parametrus. Realizuotas GaN epitaksinių sluoksnių liuminescencijos kinetikos tyrimas labai žemo kvazitolydinio sužadinimo atveju patvirtino rekombinacijos per priemaišas svarbą šio tipo medžiagose. Buvo sukurtos naujos prekinių šviestukų šiluminių savybių in-situ tyrimo metodikos, veikiančios dažninės skyros režimu. Bangos ilgio keitiklių temperatūra baltuose šviestukuose ir šilumos relaksacijos konstantos įvairaus tipo šviestukų konstrukciniuose elementuose buvo išmatuotos esant vardiniam šviestukų veikimo režimui.

Disertacija yra skirta relaksacijos procesų, vykstančių optoelektronikos medžiagose ir prietaisuose, tyrimui dažninės skyros metodu. Įprastas fluorescencijos gesimo trukmės tyrimo metodas, veikiantis harmoniškai moduliuoto žadinimo režimu, buvo adaptuotas žadinimui puslaidininkiniais šviestukais ir signalų registravimui radijo dažnių faziniu detektoriumi. Neorganiniai vandeniniu zolių-gelių metodu susintetinti fosforai, skirti baltų puslaidininkinių šviestukų gamybai, ir modernios organinės optoelektronikos medžiagos buvo ištirtos fotoliuminescencijos gesimo dėsnio ir kvantinės išeigos matavimo metodikomis, siekiant nustatyti krūvininkų rekombinacijos savybes ir optimizuoti sintezės parametrus. Realizuotas GaN epitaksinių sluoksnių liuminescencijos kinetikos tyrimas labai žemo kvazitolydinio sužadinimo atveju patvirtino rekombinacijos per priemaišas svarbą šio tipo medžiagose. Buvo sukurtos naujos prekinių šviestukų šiluminių savybių in-situ tyrimo metodikos, veikiančios dažninės skyros režimu. Bangos ilgio keitiklių temperatūra baltuose šviestukuose ir šilumos relaksacijos konstantos įvairaus tipo šviestukų konstrukciniuose elementuose buvo išmatuotos esant vardiniam šviestukų veikimo režimui. / The thesis is devoted to the frequency-resolved investigation of the relaxation processes in optoelectronic materials and devices. Conventional fluorescence decay time measurement technique in the frequency domain was adapted for the use with light-emitting diode (LED) excitation and signal registration by a lock-in amplifier. Inorganic phosphors synthesized by aqueous sol-gel combustion method for using as wavelength converters in white LEDs as well as advanced organic semiconducting materials were investigated by photoluminescence decay time and quantum yield measurement techniques. The photoluminescence decay time measurement technique with extremely low quasi-continuous UV LED excitation was applied for the carrier dynamics research in GaN epitaxial layers. The investigation under such a low excitation conditions revealed the contribution of donor-acceptor recombination in the yellow luminescence of GaN. The techniques for in-situ thermal characterization of encapsulated LEDs, including the measurements of phosphors converter temperature and heat relaxation time constants inside a LED, were developed and demonstrated for the investigation of commercial low- and high- power LEDs.