Aim of study. The purpose was to investigate and analyse mycotoxicological contamination of concentrated fodder during 2 years and to evaluate the use of chemical conserving CALPRONA NC. Main tasks: 1) To investigate the amounts of aflatoxin B1, zearelenone, T2 and deoxivalenol (DON) mycotoxins in fodder using imunoferment analysis and chromatography (TLC) method’s. 2) To determine minimum and maximum levels of mycotoxins in fodder and compare with the formative’s. 3) Compare levels of mycotoxins in fodder during period of two years. 4) Investigate the influence of chemical conserving CALPRONA NC mycostatical effect on the grain after the harvest. Material and methods: Conservated (CALPRONA NC) and non conservated samples were taken from experimental farm. Quantitative contamination of fungi (cfu – colony forming units) was determined using dilution method and Czapec Dox agar (Курасова, 1971). Level of mycotoxins was determined using IFA (imunoferment analyses) method with commercial set VERATOX®DON, T- 2 toxin, Zearalenone. Also the TLC method was applied using silicon gel plates (methology of Romer Lab.) Diffusion of mycotoxins in fodder during year 2003- 2004 was determined using processing of facts with “R” statistical pack. Results and discussion: 1) Probe fodder is contaminated with fungi and their toxins: DON, zearalenone, T- 2 and aflatoxin B1. 2) Highest levels of mycotoxins in concentrated fodder (mg/kg or ppm) are DON (0.095 ppm), comparing to zearalenone (ZON) and... [to full text]