Santrauka Antikūnų prieš Rhezus kraujo grupės sistemos antigenus atsiradimas ir paplitimas Šis tiriamasis darbas buvo atliekamas norint įvertinti antikūnų prieš Rhezus kraujo grupės sistemos antigenus atsiradimą, paplitimą, bei jų pasiskirstymą po hemotransfuzijų. Buvo ištirtas 115 recipientų kraujas, kurie gydėsi Vilniaus universiteto ligoninės Santariškių klinikose. Tiriamųjų amžiaus ribos buvo nuo 18 iki 82 metų. Antikūnų prieš Rhezus kraujo grupės sistemos antigenus nustatymas ir identifikavimas buvo atliekamas tiesioginio ir netiesioginio Kumbso metodu. Mes tyrėme antikūnų prieš Rhezus kraujo grupės sistemos antigenus aloimunizacijos riziką ketverių metų laikotarpyje. Nustatėme, kad pirmaisiais tyrimo metais šių antikūnų aloimunizacijos rizika siekė 9,56%, o tyrimo pabaigoje (2007 – 2008 m.) – 19,13%. Pirmaisiais tyrimo metais aloantikūnai prieš Rhezus kraujo grupės sistemos antigenus buvo identifikuoti 92,17%, autoantikūnai prieš Rh kraujo grupės sistemos antigenus – 7,83% pacientų. Ketvirtaisiais tyrimo metais aloantikūnai prieš Rh kraujo grupės sistemos antigenus buvo nustatyti 75,47%, o autoantikūnai prieš Rh kraujo grupės sistemos antigenus nebuvo identifikuoti nei vienam ir recipientų. Dažniausiai sutinkami Rhezus kraujo grupės sistemos aloantikūnai buvo prieš D (n = 24) ir E (n = 19) antigenus, o dažniausiai sutinkamos Rh sistemos aloantikūnų kombinacijos: anti – (D+ C) (n = 3), anti – (E+ Cw) (n = 4), anti – (E + c) (n = 3), anti – (D+ C+ E) (n = 5). Tirdami... [toliau žr. visą tekstą] / SUMMARY The Origin and the Incidence of Antibodies against Rhesus Blood System Antigens This research work was carried out seeking to assess origin and incidence of antibodies against antigens of Rhesus Blood Type system as well as their distribution after hemotransfusions. Blood of 115 recipients was explored in patients treated at Vilnius University hospital „Santariškių klinikos”. The age of the persons under research varied between 18 and 82 years. Appearance and spread of antibodies against antigens of Rhesus Blood Type system was established using the direct and indirect Coombs test. We had explored the risk of alloimmunization of antibodies against antigens of Rhesus Blood Type system within the period of four years. The risk of alloimmunization of alloantibodies against antigens of Rhesus Blood Type system, which we found on the first time within the first year of research was 9.56 %. And in the end of research, the risk of alloimmunization of alloantibodies against antigens of Rhesus Blood Type system, which we found on the first time was 19.13 %. On the whole, within the first year of research, alloantibodies against antigens of Rhesus Blood Type system were identified in 92.17 % of the patients, and the autoantibodies against antigens of Rhesus Blood Type – in 7.83 % of the patients.Within the fourth year of research, in total 75.47 % of the patients were identified alloantibodies against antigens of Rhesus Blood Type system, and the autoantibodies against antigens... [to full text]

1. For the first time a wide range of biochemical genetic markers and different typing techniques were used to access levels of genetic variability in Lithuanian horse breeds; 2. DNA based methods were used to access levels of genetic variation in Lithuanian horse breeds; 3. Genetic variation in Lithuanian horses was investigated using mitochondrial DNA (mtDNA) sequencing; 4. Genetic relationship and genetic distances between the breeds were estimated using a wide range of different genetic markers.

At present, cardiovascular diseases cause ca. 30 of deaths worldwide, and are the most common cause of death and disability (The World Health Report 2002; Pearson 1999). Coronary artery disease (CAD) accounts for nearly 50 of all deaths caused by cardiovascular diseases. In 2002, 7.2 million people died of CAD worldwide, and 5.8 million new cases were diagnosed. In 2000, the number of people with CAD around the world amounted to ca. 40 millions (Mackay 2004). The modern understanding of the pathophysiology of atherosclerosis and the concept of “cardiovascular risk factors” started forming in 1950s, when the first findings of the Framingham study were published (Wilson et al. 1998, D’Agostino et al. 2000). Information accumulated during scientific research on atherosclerosis allowed for a significant reduction of CAD-related mortality in the developed countries during the last 20 years, but a more profound analysis showed that the mortality mostly decreased in males, whereas in females it continues to grow. Nearly two-thirds of suddenly deceased women previously showed no clinical symptoms of CAD (AHA 2002). This most probably was influenced by a still predominant erroneous opinion that women, especially of younger age, very rarely have CAD and atherosclerosis of peripheral arteries. Epidemiological studies showed that cardiovascular diseases induced by atherosclerosis are equally frequent cause of death in both males and females. Of all patients who in 2000 in the U.S. died... [to full text]