Solid phase extraction (SPE) has become a popular sample preparation method for the extraction of analytes from complex matrices. Solid phase extraction overcomes such disadvantages as large solvent usage and waste, large amounts of time spent in sample preparation and labor intensive methods with little hope of automation. In this report, SPEC®⋅ Microcolumns are evaluated for the extraction of Zantac® (ranitidine hydrochloride) from urine for high performance liquid chromatography (HPLC) analysis. SPEC®⋅Microcolumns are rigid disc structures with glass fiber impregnated with silica gel. These microcolumns look like packed-bed SPE cartridges but have the advantages of low void volumes and low bed mass that give clean extracts with the use of small amounts of solvents. An SPE method was developed and evaluated for the recovery of Zantac® from water and spiked urine. Percent recoveries and matrix interferences are reported. The limit of detection and analytical range tor the HPLC method of analysis with ultraviolet detection will be presented. / Master of Science