Many lesbian, gay, bisexual, and transgender (LGBT) individuals report experiencing discrimination in their health care that leads to avoidance of regular appointments with providers. Lack of regular primary care can delay diagnoses of preventable conditions and increase patient risks for chronic disease complications. A systematic review of the literature was conducted to understand LGBT cultural competencies for nursing and other health care providers. The Cochrane Handbook for Systematic Reviews and Melnyk's levels of evidence framed this systematic literature review. Articles for inclusion were limited to those published in English between 2008 and 2018. Keywords used in the literature search included LGBT health disparity, LGBT cultural competency orientation, and nursing LGBT education. The search yielded 70 article results, which were further reduced to 12 articles by critically analyzing the applicability of the literature to the practice-related questions and removing duplicate articles. Five articles met the criteria for Levels III-IV (case-control or cohort), 6 met the criteria for Level II (randomized control trials), and 1 was Level 1 (systematic review). The analysis of evidence demonstrated the importance of providing education to nurses and other health care providers regarding LGBT cultural competency. Recommendations are offered for best practice strategies regarding the inclusion of LGBT cultural competencies in nursing orientation modules. Application of the findings may lead to positive social change if knowledgeable health care providers engage the LGBT population in primary care leading to improved health care outcomes.