This study aimed at developing allometric equations for the estimation of aboveground biomass of black willow and eastern cottonwood and determine biomass production by these species under several planting spacing and harvest frequency combinations. Logarithmic model with dbh and tree height was the best fitting model for individual tree aboveground biomass estimation of both species. At area level, logarithmic models with stand age, dominant height, and planting density produced the best results. Mixed-effects modeling showed statistically significant effects of harvest frequency for eastern cottonwood but not for black willow. Overall, we conclude that, biomass production of black willow and eastern cottonwood would play a critical role in the fulfillment of the wood energy demands and biomass yields can be enhanced by considering management factors during plantation. These findings will be useful to forest owners in Lower Mississippi Alluvial Valley for estimating biomass without destructive sampling and have optimal biomass production.