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Coupling aptamer biosensors to signal amplificationYang, Litao, 1976- 22 August 2011 (has links)
Not available / text
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Validation of a HPLC assay for porphobilinogen synthase in human erythrocytes for use in the clinical laboratorySuen, Kin-wah, 孫建華 January 2004 (has links)
(Uncorrected OCR)
Abstract
Porphobilinogen (PBG) synthase condenses two molecules of
aminolaevulinic acid (ALA) to form PBG in heme biosynthesis. The enzyme
activity is sensitive to inhibition by heavy metals such as lead. It can act as a biological indicator of chronic lead POis~r\g to identify the risk group,
especially in children, so that early treatment can be given to prevent possible
permanent damages. A reversed-phase ion-pair HPLC analytical method for
the assay of the PBG synthase activity based on detection of PBG production
has been validated. A Hypersil CN column (150 x 4.6 mm; 5 urn) was
employed together with a mixture of acetonitrile-40 mM phosphate buffer at pH
2.4 with 5 mM 1-heptanesulphonic acid (8:92, v/v). UV detection was
performed at 240 nm. PBG was eluted as a spectrally pure peak resolved from
its impurities in the methanol-inhibited enzyme reaction. The method was
sensitive with a limit of quantitation of 2 ~M. The within-run and between-run
precisions were 8.2% and 13.8% respectively. The recovery was 93.4 �7.1%
(n=6). The preliminary reference range of the PBG synthase activities in the
local pediatric population were from 21.5 to 26.3 ~mol/L RBC/min. Bland and Altman statistical analysis showed that the HPLC assay and the colorimetric assay could not be used interchangeably. The HPLC assay was an alternative way to assess the PBG synthase activities in the human erythrocyte samples.
IV / abstract / toc / Medical Sciences / Master / Master of Medical Sciences
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Development of antibody and antigen detection assays and vaccines for SARS associated coronavirusWong, Hiu-ling, Beatrice. January 2007 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2008. / Also available in print.
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64 |
Adhesion and autoaggregation of Lactobacillus reuteri and description of a new lactobacillus species with mucus binding properties /Roos, Stefan, January 1900 (has links) (PDF)
Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv. / Härtill 4 uppsatser.
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Assessing the toxic impact of chemicals using bacteria /Gabrielson, Jenny, January 2004 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst., 2004. / Härtill 4 uppsatser.
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66 |
Microbiological synthesis of riboflavin to enrich swine viscera used for poultry and livestock feed /Tylec, Fred Walter, Boyd, Vaughan Frank, January 1953 (has links)
Thesis (M.S.)--Virginia Polytechnic Institute, 1953. / Vita. Includes bibliographical references (leaves ix-xii). Also available via the Internet.
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67 |
Strategies for improving synthesis of quinic acid and shikimic acid from D-glucoseJancauskas, Justas. January 2008 (has links)
Thesis (Ph. D.)--Michigan State University. Dept. of Chemistry, 2008. / Title from PDF t.p. (viewed on Aug. 20, 2009) Includes bibliographical references. Also issued in print.
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The biosynthesis of the thiopeptide antibiotic thiostrepton /Shipley, Paul R. January 1999 (has links)
Thesis (Ph. D.)--University of Washington, 1999. / Vita. Includes bibliographical references (leaves 148-159).
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69 |
Genetic methods for rapid detection of medically important nosocomial bacteriaThomas, Lee Carolyn. January 2007 (has links)
Thesis (M. Sc. Med.)--University of Sydney, 2007. / Title from title screen (viewed 15 October 2008). Submitted in fulfilment of the requirements for the degree of Master of Science in Medicine to the Discipline of Medicine, Faculty of Medicine. Includes bibliographical references. Also available in print form.
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Application of flow injection in the assay of selected substances in food and clinical analysisMulaudzi, Ludwig Vusimuzi. January 1999 (has links)
Thesis (M.Sc.(Chemistry))--University of Pretoria, 1999. / Includes abstract in English and Afrikaans. Includes bibliographical references.
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