>Magister Scientiae - MSc / The Karoo in South Africa is an area in which petroleum studies are being reviewed with particular interest, especially within the Tanqua and Laingsburg subbasins of the Karoo. The particular formations of interest comprises of the Laingsburg, Fort Brown, Kookfontein and Vischkuil of the Upper Ecca group, located within the Laingsburg subbasin as well as the Kookfontein formation found within the Tanqua subbasin. The present work dealt with interpreting and distinguishing the different types of source rock lithologies, to identify the source rock zones and organic matter within the Laingsburg, Fort Brown, Kookfontein and Vischkuil formations. This was achieved by utilising different geochemical analyses (such as rock eval pyrolysis and TOC) and organic chemistry (XRD) to assess the maturity, mineralogy and the quality of the source rocks.