Laminar flame speed of a novel liquid bio-fuel has been determined experimentally using a closed spherical combustion vessel of 29 L equipped with two pairs of fused silica windows for optical access at atmospheric pressure and elevated temperature conditions. Schlieren technique was used to visualize and record the temporal evolution of the outwardly spherical flame front, and an in-house developed Matlab code was employed to process the flame front images and calculate its area by applying several image processing techniques. The test conditions consisted of varying the fuel-air mixture equivalence ratio at atmospheric standard pressure and different initial temperatures. Validation of the present method was achieved by measuring and comparing the flame speed of methane/air and n-heptane/air mixture with their published counterparts. Experimental results revealed comparable laminar flame speed of the novel liquid biofuel (1, 3- dimethoxyoctane) to heavy liquid hydrocarbons such as n-heptane and isooctane, especially at stoichiometric and fuel rich conditions. Additionally, the flammability limits of this novel fuel showed similarities with those of gaseous hydrocarbons fuels (e.g. methane, ethane) but higher than those of liquid hydrocarbons (e.g. diesel, gasoline). / February 2016