Hurricane Isaac’s landfall on the coast of Louisiana spawned a hydrological research project between Mississippi State University (MSU), the Northern Gulf Institute (NGI), and the National Oceanic and Atmospheric Administration (NOAA) in the Lower Pearl River Basin (LPRB). Unmanned aerial systems data collection missions were scheduled every two months in the LPRB. This research provides a comparison between Landsat-8 imagery and corresponding UAS imagery with regards to the four remote sensing resolutions: spatial, spectral, radiometric, and temporal. Near-infrared (NIR) imagery from each platform was compared by land-water masks and statistical comparisons. A classification method known as natural breaks with Jenks Optimization determined threshold values between land and water for each image. Land-water masks revealed substantial differences between areas of land and water in comparing imagery. The overall difference in average land and water percentages between the two platforms was 1.77%; however, a larger percentage was 20.41% in a single comparison.