Doctor of Philosophy / Department of Horticulture and Natural Resources / Jack Fry / Megan Kennelly / Zoysiagrasses (Zoysia spp.) use C4 metabolism and are more drought resistant than C3 grasses. However, the long dormancy period between autumn and spring limits the use of zoysiagrass by homeowners and professional turfgrass managers. In addition, large patch has become the primary pest on zoysiagrass, and improved cultivars with good cold hardiness and large patch resistance are needed in the transition zone. Tall fescue (Schedonorus arundinaceus Schreb), a C3 grass, is used frequently in Kansas due to its heat and drought tolerance compared to some other C3 grasses. However, brown patch (Rhizoctonia solani) is the main disease limiting its growth in summer. Alternatively, mixing zoysiagrass with tall fescue may help reduce brown patch incidence. The objective of these projects were to: (1) evaluate methods for establishing a perennial mixture of seeded zoysiagrass and tall fescue; (2) determine whether a zoysiagrass/ tall fescue polystand is less susceptible to brown patch and results in improved summer quality compared to a tall fescue monostand; and (3) evaluate experimental zoysiagrass genotypes to identify one or more potential new cultivars which have high quality and tolerance to cold and large patch. I found that polystands of zoysiagrass and tall fescue were most successfully established by seeding zoysiagrass at 49 kg ha-1 in June and tall fescue at 392 kg ha-1 in September into the established zoysiagrass sward. Polystand establishment was also superior at a 1.9 cm mowing height than a 5.1 cm mowing height. The resulting mixture resulted in improved turf color in late fall and early spring compared to a zoysiagrass monostand. In addition, using a zoysiagrass-tall fescue polystand reduced brown patch by up to 21% compared to a tall fescue monostand. In the zoysiagrass breeding project, I identified ten progeny out of sixty evaluated that had better tolerance to large patch (up to 40 % less plot area affected) and better quality compared to Meyer zoysiagrass, which is the standard cultivar used in the transition zone.

Doctor of Philosophy / Department of Plant Pathology / Megan Kennelly / Large patch, caused by the fungus Rhizoctonia solani anastomosis group (AG) 2-2 LP, is the most common and severe disease of zoysiagrass (Zoysia spp). Despite the importance of this disease, few studies have examined pathogen biology, cultivar susceptibility, cultural controls, and chemical controls. The objectives of this dissertation were: (1) Characterize large patch isolates based on anastomosis pairing, in-vitro mycelial growth rates, nuclear counts, virulence, PCR, and amplified fragment length polymorphism (AFLP); (2) Determine the effects of cultivation (aerification, verticutting, and sand topdressing) on disease severity; (3) Evaluate different fall and spring applications of the fungicides flutolanil, azoxystrobin, and triticonazole; (4) Evaluate the susceptibility of fifteen new zoysiagrass germplasm lines from parental crosses including Z. japonica, Z. matrella, and Z. pacifica. All the R. solani isolates from large patch-infected zoysiagrass from Kansas belonged to AG 2-2 LP. Variations were observed among the isolates in their average number of nuclei per cell, mycelial growth rates and virulence. There was also variation in the amplified fragment length polymorphism (AFLP) DNA fingerprints, suggesting possible underlying genetic differences of biological significance among members of AG 2-2 LP. Cultivation did not affect soil moisture or temperature. Cultivation also did not reduce patch sizes, nor influence turf recovery rate from large patch. From 2009 to 2011, spring and fall N fertility was consistently associated with lower percentages of diseased turf in both cultivated and non-cultivated plots at Manhattan and Haysville. In general, two fall applications of fungicide did not reduce disease compared to one fall application. Fungicides applied in the fall when thatch temperatures ranged from 17.8oC to 23.2oC reduced disease compared to untreated controls. Early spring applications reduced disease compared to later spring applications. In germplasm screening studies, all progeny had similar disease levels compared to Meyer in the growth chamber, but only 6 consistently had disease levels as low as Meyer in the field. Growth chamber results did not correlate to field results.