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Complete sequence, improved detection and functional analysis of Grapevine Leafroll-associated Virus 1(GLRaV-1) / by Alan Little.Little, Alan January 2004 (has links)
"April, 2004" / "List of figures" - inside back cover. / Bibliography: leaves 83-93 / 93, [8] leaves : ill., plates (some col.), photos ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / The specific objectives of this work include: 1. Completion of the GLRaV-1 genome sequence and bioinformatic analysis of the viral open reading frames ; 2. Production of an appropriate GLRaV-1 certification protocol addressing the shortcomings of the current tests for leafroll detection ; 3. Intracellular localisation of the GLRaV-1 gene products via generation of green fluorescent protein (GFP)-fusion constructs, in an attempt to further characterise the function of these proteins. / Thesis (Ph.D.)--University of Adelaide, School of Agriculture and Wine, Discipline of Wine and Horticulture, 2004
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Complete sequence, improved detection and functional analysis of Grapevine Leafroll-associated Virus 1(GLRaV-1) /Little, Alan. January 2004 (has links) (PDF)
Thesis (Ph.D.)--University of Adelaide, School of Agriculture and Wine, Discipline of Wine and Horticulture, 2004. / "April, 2004" "List of figures" - inside back cover. Bibliography: leaves 83-93.
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Factors affecting green peach aphid populations on potatoes and the transmission of potato leafroll virusFerguson, James Scott. January 1980 (has links)
Thesis (M.S.)--University of Wisconsin--Madison, 1980. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 95-102).
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Systemic insecticides in relation to the transmission of potato leafroll virus by Myzus persicae SulzerVillacarlos, Lina Teneza. January 1983 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison,1983. / Typescript. Vita. Description based on print version record. Includes bibliographical references (leaves 146-159).
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Studies on potato leaf roll virusHepp, Ruperto F. January 1976 (has links)
Thesis (Ph. D.)--University of Wisconsin-Madison, 1976. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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A study of luteoviruses involved in potato leafroll diseaseEllis, Peter John January 1991 (has links)
In total, 801 samples of potato leafroll disease were collected and tested for potato leafroll virus (PLRV) and beet western yellows virus (BWYV) in 1986, 1987, and 1988 using triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) and virus-specific monoclonal antibodies. The samples represented 32 cultivars and originated in eight Canadian provinces and 12 American states. None of the samples tested positive for BWYV, whereas 772 (96.4%) tested positive for PLRV. Neither PLRV nor BWYV could be recovered, with aphid transfers to indicator hosts, from 28 of the 29 samples that tested negative for both viruses. PLRV was recovered from one sample that originally tested negative by TAS-ELISA; the indicator plant tested positive for PLRV by TAS-ELISA.
Nucleic acid spot hybridization (NASH) using random primed and cloned cDNA probes was compared with double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and TAS-ELISA, and aphid transmission tests for detection and identification of PLRV and BWYV in 165 potato leafroll disease samples. All of the samples tested negative for BWYV with each of the assay procedures. PLRV was detected in all of the samples with TAS-ELISA, NASH with a cloned cDNA probe for PLRV, and with aphid transmission to ground cherry (Physalis
pubescens). Both DAS-ELISA and NASH using random primed cDNA produced one false-negative result. Shepherd's purse (Capsella bursa-pastoris) was a host for 72% (119/165) of the PLRV isolates.
The susceptibility of potato to BWYV was tested by inoculating Russet Burbank with three isolates of BWYV from Canada and four from the United States. Two of the isolates were in a mixed infection with PLRV. None of the isolates were transmitted by Myzus persicae to virus-free potato plants, either by themselves or in association with PLRV.
Common weeds were surveyed in the potato-producing areas of British Columbia for PLRV and BWYV. In total, 10,098 weed samples, representing 98 species in 22 plant families, were collected and tested by TAS-ELISA from 1986 to 1989. BWYV was detected in 1% of the samples; the hosts were: chickweed, common groundsel, heart-podded hoary cress, hedge mustard, little western bittercress, prickly lettuce, shepherd's purse, and wild radish. PLRV was detected in three volunteer potato plants, two samples of shepherd's purse, and one black nightshade plant. The low incidence of PLRV in plants other than potato indicates that weeds are of minor importance in the epidemiology of potato leafroll disease in British Columbia. / Land and Food Systems, Faculty of / Graduate
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Imunologická a molekulární diagnostika viru svinutky bramboruKrédl, Zdeněk January 2008 (has links)
No description available.
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Potato diseases in South Australia : studies in leafroll, early blight and bacterial wilt /Akiew, E. B. January 1985 (has links) (PDF)
Thesis (Ph. D.)--University of Adelaide, Dept. of Plant Pathology, 1985. / Includes bibliographical references (leaves 119-127).
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Influence of hairy nightshade, Solanum sarrachoides (Sendtner) on the Potato leafroll virus pathosystem /Srinivasan, Rajagopalbabu. January 1900 (has links)
Thesis (Ph. D.)--University of Idaho, 2006. / Abstract. "May 2006." Includes bibliographical references. Also available online in PDF format.
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An assessment of the mutation patterns in South African isolates of Potato leafroll virus and the expression of recombinant viral coat protein genes in Escherichia coliRothmann, Adri Hilda 03 1900 (has links)
Thesis (MSc)--University of Stellenbosch, 2007. / ENGLISH ABSTRACT: Presently, the observed variation in symptoms of Potato leafroll virus (PLRV) infection in potato
cultivars in South Africa cannot be reconciled with PLRV symptoms obtained 10-15 years ago,
even if the different interactions between the pathogen and the cultivar are taken into account.
In an effort to analyze this variation, mutations in the coat protein (CP) gene of South African
isolates of PLRV were assessed. The CP gene of PLRV isolates from different areas within
South Africa was amplified by reverse transcription-polymerase chain reaction (RT-PCR),
cloned and sequenced. Significant sequence variation in the CP gene was found within the
analyzed South African isolates of PLRV. Phylogenetic analysis revealed two major clades with
most South African isolates and an Australian and North American isolate grouped together and
the remainder grouped with isolates from diverse countries worldwide. The deduced amino acid
sequences from representatives of these two clades indicated differences in CP threedimensional
structure.
In an effort to produce recombinant PLRV CP for the production of antibodies specific for South
African isolates of PLRV for use in enzyme-linked immunosorbent assay (ELISA), the CP gene
of a South African isolate of PLRV was subcloned into a bacterial expression vector (pET14-b).
Expression of full length recombinant PLRV CP was attempted in Escherichia coli strains
BL21(DE3)pLysS, Rosetta-gami B(DE3)pLysS and Rosetta-2(DE3)pLysS. As this was not
successful, the PLRV CP gene was subcloned in another expression vector (pGEX) for
expression as an N-terminal fusion protein with glutathione-S-transferase (GST) in E. coli
strains BL21(DE3)pLysS and Rosetta-2(DE3)pLysS. The recombinant GST-PLRV CP fusion
protein was purified and used for antibody production in rabbits. Using western blots, the
effectiveness of antibodies produced to recombinant GST-PLRV CP fusion protein was
assessed for PLRV recognition. It was found that antibodies to the recombinant GST-PLRV CP
fusion protein were more effective for the detection of GST than PLRV CP and that production
of antibodies to the cleaved PLRV CP product would be necessary if antibodies are required for
ELISA applications. / AFRIKAANSE OPSOMMING: Huidiglik kan die waargeneemde simptome van infeksie met aartappelrolbladvirus (Potato
leafroll virus, PLRV) in aartappelkultivars in Suid-Afrika nie vereenselwig word met PLRV
simptome wat 10-15 jaar gelede verkry was nie, selfs al word die verskillende interaksies tussen
die patogeen en kultivar in ag geneem.
In ‘n poging om hierdie variasie te analiseer, was mutasies in die mantelproteïen (CP) geen van
Suid-Afrikaanse isolate van PLRV bepaal. Die CP geen van PLRV isolate van verskillende
areas in Suid-Afrika was ge-amplifiseer met behulp van die tru transkripsie-polimerase ketting
reaksie (RT-PCR), gekloneer en die nukleotiedvolgorde bepaal. Noemenswaardige nukleotied
variasie is in die CP gene van die ge-analiseerde Suid-Afrikaanse isolate van PLRV gevind.
Filogenetiese analises het gedui op twee hoof klades met die meeste van die Suid-Afrikaanse
isolate wat saam met ‘n Australiese en Noord-Amerikaanse isolaat gegroepeer en die res wat
met isolate van verskillende lande wêreldwyd gegroepeer. Die afgeleide aminosuurvolgordes
van verteenwoordigers van bogenoemde twee klades het gedui op verskille in die CP driedimensionele
struktuur.
In ‘n poging om rekombinante PLRV CP te produseer vir die produksie van antiliggame
spesifiek teen Suid-Afrikaanse isolate van PLRV om in “enzyme-linked immunosorbent assay”
(ELISA) te gebruik, was die CP geen van ‘n Suid-Afrikaanse isolaat van PLRV gesubkloneer in
‘n bakteriële ekspressie vektor (pET14-b). Daar was gepoog om vollengte rekombinante PLRV
CP in die Escherichia coli rasse BL21(DE3)pLysS, Rosetta-gami B(DE3)pLysS en Rosetta-
2(DE3)pLysS te produseer. Aangesien dit nie suksesvol was nie, was die PLRV CP
gesubkloneer in ‘n ander ekspressie vektor (pGEX) sodat die proteïen as ‘n N-terminale fusie
proteïen met “glutathione-S-transferase” (GST) in E. coli rasse BL21(DE3)pLysS en Rosetta-
2(DE3)pLysS geproduseer kon word. Die rekombinante GST-PLRV CP fusie proteïen was
gesuiwer en gebruik vir antiliggaam produksie in konyne. Die effektiwiteit van die antiliggame
wat teen rekombinante GST-PLRV CP fusie proteïen geproduseer was vir PLRV herkenning is
deur middel van “western blots” geanaliseer. Dit was gevind dat antiliggame teen die
rekombinante GST-PLRV CP fusie proteïen meer effektief was vir die herkenning van GST as
PLRV CP. Gevolglik sal dit nodig wees om antiliggame teen die gesnyde PLRV CP produk te
maak vir gebruik in ELISA.
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