The applicability of trauma studies within an examination of postcolonial literature has been a contested topic for scholars in both fields. Additionally, scholarship regarding Amitav Ghosh’s postcolonial science fiction novel The Calcutta Chromosome encourages various readings of the novel but does not currently offer a cohesive examination of all its thematic disciplines and stylistic elements. Through an examination of this postcolonial novel, I will provide a more holistic reading of the novel through an application of trauma studies that explores its representation of the internal postcolonial conflict regarding Western and non-Western historiographies. My analysis will focus on the lexical, character, and narrative levels of the novel through its dominant medical, technological, postcolonial, and political themes for inclusions of Caruth’s aporia related to the manifestation of trauma in literature.