The effects of light, light "mimicking" chemicals, and protein synthesis inhibitors on the photo-induced carotenogenesis of Corynebacterium 7EIC were studied. Changes in the dosage of fluorescent light applied to dark grown cells showed a dose related carotenogenic response. Maintaining the same dosage but varying the wavelength of monochromatic light revealed that light with a wavelength of 280 to 450nm was responsible for photo-induction. It further showed a peak of photo-induction between the wavelengths of 370 and 430nm. The light "mimicking" chemicals antimycin A and p-Chloromercurybenzoate were shown to have no light "mimicking" effects. The transcriptional inhibitor of protein synthesis actinomycin D partially inhibited, and chloramphenicol a translational inhibitor, completely inhibited photo-induced carotenogenesis.

The expression of photorefractoriness, a feature of seasonal breeding in birds, is important in production species, where egg production and fertility are affected by the photoperiod. Years of selection in meat-type birds have reduced the potential reproductive function, and the environmental manipulation of such genotypes is essential to maximise productivity. While it is known that egg-type pullets no longer exhibit photorefractoriness, and that female broiler breeders do, there is not much information about the response of male broiler breeders to photoperiod. Such information is important when designing lighting programmes for breeding stock, and this study aimed to gain more insight into the male response to photoperiod. A series of experiments was designed in order to achieve this. In the first the response of male broiler breeders to rearing on constant photoperiods was measured, and in the second the effects of age at photostimulation on age at sexual maturity were assessed. The response to an increase in photoperiod during the production cycle was investigated in the third trial. Evidence of photorefractoriness in males was observed, but this was not the case in all birds, possibly due to high variation in some of the fertility traits measured. It seems possible that males do not respond to photoperiod to the same extent as females. Unexpected observations of a response in some birds to early photostimulation, and the high variation observed prompted another experiment in which the heritability of the response to early photostimulation was measured as a potentially revolutionary method of selection, whilst checking that this response is not negatively linked to broiler growth rates. Left and right testis weight data, collected in the above experiments, were analysed for asymmetry. There was no consistent response in testes asymmetry to photoperiod, or evidence that asymmetry in testis weights is as a result of the imposition of genetic or environmental stress. The strong correlation found between testes dimensions and weight suggests that predictions of testis weights could be made through ultrasonics or laparoscopy. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

No abstract available. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2008.

Lo Chi-yan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (leaves 42-51). / Abstract in English and Chinese; questionnaires in Chinese. / Abstract --- p.i / Acknowledgments --- p.iv / Table of Contents --- p.v / Chapter CHAPTER 1 --- Introduction --- p.1 / Why is empirical research on driver sleepiness important? --- p.2 / Prevalence of driver sleepiness --- p.2 / Driver sleepiness as a risk factor for traffic accidents --- p.3 / Why should taxi driver sleepiness be studied? --- p.6 / Variations in sleepiness and vigilance and driving performance within a shift and comparison between daytime and nighttime drivers --- p.8 / "The impact of circadian typology on sleep, sleepiness, vigilance and driving performance" --- p.11 / Bright light exposure --- p.14 / Overview of the present study --- p.18 / Chapter CHAPTER 2 --- Main study --- p.20 / Recruitment and Characteristics of Participants --- p.20 / Procedure --- p.20 / Instruments --- p.23 / Chapter CHAPTER 3 --- Results --- p.28 / Chapter CHAPTER 4 --- Discussion --- p.36 / References --- p.42 / Figures --- p.52 / Tables --- p.55 / Appendices --- p.62

Indiana University-Purdue University Indianapolis (IUPUI) / The phototropin photoreceptors are important regulators of plant growth and development and can therefore affect the photosynthetic activity of plants. Phototropin1 and Phototropin2 are versatile protein kinases that become activated when exposed to blue light. Their photobiological actions are best understood in the model plant Arabidopsis thaliana, where they are known to trigger several responses to blue light, one of which is phototropism, the bending of plant organs towards light. Additionally, phot1 and phot2 drive stomatal opening, chloroplast arrangement in leaf cells, leaf expansion, and leaf orientation. The phot1-specific response is rapid inhibition of hypocotyl growth, leaf positioning and mRNA stability whereas phot2 mediates the chloroplast avoidance response to high light. These responses impact a plant’s ability to capture light for photosynthesis, therefore the phototropins play important roles in optimizing a plant’s photosynthetic activity. Soybean (Glycine max) is a very important crop plant in Indiana known for its nutritional versatility and is also utilized for biodiesel production.In spite of soybean being a key crop, there is currently no information about the functionality of soybean phototropins. Also, being a legume, soybean has many structural and functional features that are not present in Arabidopsis. Interestingly, PsPHOT1A (a photoreceptor from garden pea) was found to be a functional phototropin as it was able to complement the phot1 mutation in Arabidopsis. The roles of these proteins in soybean will be elucidated based on the hypothesis that soybean phototropins play essential roles in regulating photosynthetic activity as do the Arabidopsis phototropins. To date, five soybean phototropins, 3 PHOT1s and 2 PHOT2s, are believed to exist. These GmPHOT protein coding regions were amplified by RT-PCR and cloned into pCR8/TOPO or pENTR-D/TOPO vectors via TOPO cloning to utilize Gateway cloning technology to create plant transformation constructs subsequently. The cloned GmPHOT cDNAs from each of the 5 GmPHOTs were sequenced and compared to the GmPHOT sequences from the Phytozome database to assess the accuracy of the gene models. The gene models of all the GmPHOTs were found to be accurate except that of GmPHOT1B-2. The high level of sequence identity between the GmPHOTs and AtPHOTs and the conservation of LOV domains and catalytic domains indicate structural resemblance between them. This suggests that soybean phototropins should encode active photoreceptors. The cloned protein coding regions from soybean were then recombined into a plant expression vector via Gateway technology,which were then used for transformation of Agrobacterium tumefaciens. These plant expression constructs will be utilized in the future to determine the functionality of soybean phototropins in Arabidopsis.