Listeria monocytogenes response to oxidative stress by sublethal sodium hypochlorite was investigated in this study. Continuous exposure of sublethal chlorine influenced biofilm formation and stress adaptation (homologous and heterologous) in L. monocytogenes. The biofilm forming ability of oxidative stress adapted and control cells were investigated on polystyrene surface at 22°C and 37°C. The oxidative stress adapted cells were found to form less biofilm in the presence of chlorine (p < 0.10) when compared to non-treated control cells at both the temperatures. In addition, the biofilm forming ability of L. monocytogenes was reduced significantly at higher sublethal chlorine concentrations (p < 0.10). In conclusion, oxidative stress adapted L. monocytogenes has developed tolerance to chlorine and some of the antibiotics. However, oxidative stress those cells did demonstrate an antibiofilm effect. This demonstrates that oxidative stress reduces L. monocytogenes biofilm formation but can also increase antibiotic resistance.