Thesis (M.Sc. (Ichthyology & Fisheries Science)) - Rhodes University, 2009.

A stress-induced increase in metabolic rate of fish consumes energy within the metabolic scope of a fish that could otherwise be used for such functions as growth and reproduction. By estimating the degree of the metabolic response under given salinity levels and sudden changes thereof, it could be tested whether growth under given culture conditions could be predicted. Using intermittent respirometers, this study investigated the metabolic response of juvenile Lithognathus lithognathus following gradual acclimation to 5, 25 and 35‰ and, as a result of abrupt change from 35‰ to 5‰ or from 35‰ to 25‰ at 20˚C. The main aim of the study was to establish whether the magnitude of such responses could be used to predict growth of juvenile L. lithognathus under culture conditions. Hence, in addition to the respirometry study, two growth studies were conducted at 5, 10, 25 and 35‰ salinities. The baseline metabolic rates of juvenile L. lithognathus were also determined. Oxygen consumption measurements over 24-hours showed that most fish exhibited a diurnal peak in metabolic rates. The standard and active metabolic rates calculated from juvenile L. lithognathus with a diurnal peak in oxygen consumption were 0.06±0.001mgO₂g⁻¹h⁻¹ (mean±SEM, n = 5), and 0.11±0.01mg O₂g⁻¹h⁻¹, respectively. The standard and active metabolic rates of juvenile L lithognathus showing a nocturnal peak in metabolic activities were 0.04±0.001mgO₂g-1h-1 (n = 1), and 0.12±0.003 mg O₂g⁻¹ h⁻¹, respectively. Routine metabolic rate of these fish calculated over a 3-h measurement period was 0.09±0.005mgO₂g⁻¹h⁻¹ (n = 6). Juvenile L. lithognathus showed a relationship between metabolic rate (mo₂) and body weight (W) following the equation: mo₂ = 0.62 W⁻°·⁵³.

White steenbras Lithognathus lithognathus (Pisces: Sparidae) has been a major target species of numerous fisheries in South Africa, since the late 19th century. Historically, it contributed substantially to annual catches in commercial net fisheries, and became dominant in recreational shore catches in the latter half of the 20th century. However, overexploitation in both sectors resulted in severe declines in abundance. The ultimate collapse of the stock by the end of the last century, and the failure of traditional management measures to protect the species indicate that a new management approach for this species is necessary. The species was identified as a priority for research, management and conservation in a National Linefish Status Report. Despite knowledge on aspects of its biology and life history, little is known about juvenile habitat use patterns, home range dynamics and movement behaviour in estuaries. Similarly, the movement and migration of larger juveniles and adults in the marine environment are poorly understood. Furthermore, there is a complete lack of information on its genetic stock structure. Such information is essential for effective management of a fishery species. This thesis aimed to address the gaps in the understanding of white steenbras movement patterns and genetic stock structure, and provide an assessment of its current conservation status. The study adopted a multidisciplinary approach, incorporating a range of methods and drawing on available information, including published literature, unpublished reports and data from long-term monitoring programmes. Acoustic telemetry, conducted in a range of estuaries, showed high site fidelity, restricted area use, small home ranges relative to the size of the estuary, and a high level of residency within estuaries at the early juvenile life stage. Behaviour within estuaries was dominated by station-keeping, superimposed by a strong diel behaviour, presumably based on feeding and/or predator avoidance, with individuals entering the shallow littoral zone at night to feed, and seeking refuge in the deeper channel areas during the daytime. Conventional dart tagging and recapture data from four ongoing, long-term coastal fish tagging projects, spread throughout the distribution of this species, indicated high levels of residency in the surf zone at the late juvenile and sub-adult life stages. Consequently, juvenile and sub-adult white steenbras are vulnerable to localised depletion, although they can be effectively protected by suitably positioned estuarine protected areas (EPAs) and marine protected areas (MPAs), respectively. It has been hypothesized that adult white steenbras undertake large-scale coastal migrations between summer aggregation areas and winter spawning grounds. The scale of observed coastal movements was correlated with fish size (and age), with larger fish undertaking considerably longer-distance coastal movements than smaller individuals, supporting this hypothesis. Given the migratory behaviour of adults, and indications that limited spawning habitat exists, MPAs designed to protect white steenbras during the adult life stage should encompass all known spawning aggregation sites. The fishery is plagued by problems such as low compliance and low enforcement capacity, and alternative management measures, such as seasonal closure, need to be evaluated. Despite considerable conventional dart tagging effort around the coastline (5 782 fish tagged) with 292 recaptures there remains a lack of empirical evidence of fish migrating long distances (> 600 km) between aggregation and spawning areas. This uncertainty in the level of connectivity among coastal regions was addressed using mitochondrial DNA sequencing and genotyping of microsatellite repeat loci in the nuclear genome, which showed no evidence of major geographic barriers to gene flow in this species. Samples collected throughout the white steenbras core distribution showed high genetic diversity, low genetic differentiation and no evidence of isolation by distance or localised spawning. Although historically dominant in several fisheries, analysis of long-term commercial and recreational catch data for white steenbras indicated considerable declines and ultimately stock collapse. Improved catch-per-unit-effort in two large MPAs subsequent to closure confirmed that MPAs can be effective for the protection of white steenbras. However, the current MPA network encompasses a low proportion of sandy shoreline, for which white steenbras exhibits an affinity. Many MPAs do not prohibit recreational shore angling, which currently accounts for the greatest proportion of the total annual catch. Furthermore, EPAs within the juvenile distribution protect a negligible proportion of the total available surface area of estuaries – habitat on which white steenbras is wholly dependent. Despite some evidence of recent increases in abundance in estuaries and the surf zone in certain areas, white steenbras meets the criteria for “Endangered” on the IUCN Red List of Threatened Species, and for “Protected species” status on the National Environmental Management: Biodiversity Act of South Africa. The species requires improved management, with consideration for its life-history style, estuarine dependency, surf zone residency, predictable spawning migrations and its poor conservation status. The multidisciplinary approach provides valuable information towards an improved scientific basis for the management of white steenbras and a framework for research that can be adopted for other overexploited, estuarine-associated coastal fishery species.