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Signficance of cell cycle regulators in human hepatocellular carcinomaand gene expression induced by cisplatin in hepatoma cell linesQin, Lanfang., 秦蘭芳. January 2000 (has links)
published_or_final_version / Pathology / Doctoral / Doctor of Philosophy
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Overexpression of PAK4 and its relevance in hepatocellular carcinomaXu, Haitao, 許海濤 January 2006 (has links)
published_or_final_version / abstract / Pathology / Master / Master of Philosophy
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Characterization of mitotic checkpoint proteins, MAD1 and MAD2, in hepatocellular carcinomaSze, Man-fong., 施敏芳. January 2006 (has links)
published_or_final_version / abstract / Pathology / Doctoral / Doctor of Philosophy
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Epigenetic inactivation and tumor suppressive roles of hepatocyte growth factor activator inhibitors(HAIs) in human hepatocellularcarcinomaTung, Kwok-kwan., 董國焜. January 2007 (has links)
published_or_final_version / Pathology / Doctoral / Doctor of Philosophy
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Epigenetic regulation of gene expression of cystatin 6, CST6, in hepatocellular carcinomaMa, Ka-li, Marcella, 馬嘉莉 January 2005 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
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Identification of miR-106b over-expression in metastatic hepatocellular carcinoma by using the orthotopic animal modelYau, Wing-lung., 邱泳龍. January 2010 (has links)
published_or_final_version / Surgery / Doctoral / Doctor of Philosophy
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Expression of met receptor tyrosine kinase in hepatocellularcarcinomaCheung, Man-ting., 張敏婷. January 2011 (has links)
published_or_final_version / Pathology / Master / Master of Medical Sciences
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MicroRNAs associated with granulin-epithelin precursor in hepatocellular carcinomaLau, Pok, 劉博 January 2014 (has links)
Hepatocellular carcinoma (HCC) is the major type of liver cancer. In Hong Kong, thousands of deaths are related to this disease every year. Hepatitis B virus (HBV) infection is one of the major risk factors of HCC development. The high prevalence of HBV carriers in Southeast Asia including Hong Kong can account for the particularly high HCC cases in these areas.
HCC is often asymptomatic. The diagnosis and treatment are often delayed which lead to inapplicable of surgical resection. Meanwhile, conventional treatment regimes such as systemic chemotherapy were found to have limited responses. Hence, the case mortality rate of HCC is the second highest among all the cancers.
Granulin-epithelin Precursor (GEP) is a glycoprotein growth factor which regulates multiple cellular functions. Our group has demonstrated that GEP is over-expressed in more than 70% of HCC cases and GEP expression is positively correlated to tumor malignancy. Our group has also verified that suppression of GEP by monoclonal antibody leads to significant inhibition of HCC growth and reduction of malignancy. Therefore, GEP has the potential to be a novel therapeutic target of HCC.
MicroRNAs (miRNAs) are short non-coding RNAs that regulate mRNA translation. Previous studies showed that miRNA dys- regulation is closely associated with HCC progression and the high stability of miRNAs allows them to be cancer biomarkers or therapeutic targets. This project aims to investigate the miRNAs that regulate GEP and their functions in HCC.
Potential GEP-regulating miRNAs were identified by literature review and in silico prediction by bioinformatics tools. MiR-615-5p, miR-588, miR-29b, miR-195, and miR-659 were identified as the potential candidates. Quantitative polymerase chain reaction (qPCR) was utilized to examine the miRNAs’ expressions in HCC clinical samples. Only miR-29b and miR-195 were detected and hence they were selected for further study.
Our results showed that miR-29b and miR-195 expression levels were significantly decreased in HCC comparing to adjacent non‐tumor tissue (P<0.001) in more than 70% of cases.
MiR‐195 and miR‐29b were over‐expressed in Hep3B HCC cell lines by miRNA mimics and GEP protein level was significantly suppressed after miR-29b mimic transfection. The transcript level of GEP was found to be unchanged after the miR‐29b over-expression. This suggests miR‐29b does not regulate GEP protein expression by mRNA degradation.
The effects of miR‐195 and miR‐29b on HCC proliferation were also examined. The growths of HCC cells were suppressed notably after over-expression of miR‐195 (P<0.005) and miR‐29b (P<0.005) respectively.
In conclusion, miR‐195 and miR‐29b are frequently down-regulated in HCC. MiR‐29b can negatively regulate GEP expression and does not interfere with GEP mRNA level. Furthermore, miR‐195 and miR-29b can function to inhibit HCC cell growth significantly. / published_or_final_version / Surgery / Master / Master of Philosophy
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Significance of the granulin-epithelin precursor (GEP) gene in hepatocellular carcinomaWong, San-yu, Ashley., 王新愉. January 2003 (has links)
published_or_final_version / abstract / toc / Surgery / Master / Master of Philosophy
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SPARC and SPARC-like 1 are associated with tumor angiogenesis in hepatocellular carcinomaLau, Pik-yuk, Cecilia., 劉碧玉. January 2004 (has links)
published_or_final_version / abstract / toc / Surgery / Master / Master of Philosophy
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