Spatial analysis methods used for detecting, interpolating or predicting local patterns require a delineation of a neighbourhood defining the extent of spatial interaction in geographic data. The most common neighbourhood delineation techniques include fixed distance bands, k-nearest neighbours, or spatial adjacency (contiguity) matrices optimized to represent spatial dependency in data. However, these standard approaches do not take into consideration the geographic or environmental constraints such as impassable mountain ranges, road networks or coastline barriers. Specifically, complex marine landscapes and coastlines present common problematic neighbourhood definitions for standard neighbourhood matrices used in the spatial analysis of marine environments. Therefore, the goal of our research is to present a new approach to constraining spatial neighbourhoods when conducting geographical analysis in marine environments. To meet this goal, we developed methods and software (COnstraining SpatIal NEighbourhoods - COSINE) for modifying spatial neighbourhoods, and demonstrate their utility in two case studies. Our method enables delineation of neighbourhoods that are constrained by coastlines and the direction of marine currents. Our software calculates and evaluates whether neighbouring features are separated by land, or are within a user defined angle that excludes interaction based on directional processes. Using decision rules a modified spatial weight matrix is created, either in binary or row-standardized format. Within open source software (R), a graphical user interface enables users to modify the standard spatial neighbourhood definition distance, inverse distance and k-nearest neighbour. Two case studies are presented to demonstrate the usefulness of the new approach for detecting spatial patterns: the first case study observes marine mammals’ abundance and the second, oil spill observation. Our results indicate that constraining spatial neighbourhoods in marine environments is particularly important at larger spatial scales. The COSINE tool has many applications for modelling both environmental and human processes. / Graduate / 0463 / 0366 / suarezc@uvic.ca