No abstract available / Dissertation (MSc)--University of Pretoria, 2013. / am2013 / Genetics / unrestricted

As part of a vegetation survey program for the newly acquired farms incorporated into the Loskop Dam Nature Reserve, the vegetation of the Hondekraal Section was investigated. The study provides an ecological basis for establishing an efficient wildlife management plan for the Reserve. From a TWINSPAN classification, refined by Braun-Blanquet procedures, 12 plant communities, which can be grouped into eight major plant communities, were identified. A classification and description of the major plant communities are presented as well as a management plan. Descriptions of the plant communities include characteristic species as well as prominent and less conspicuous species of the tree, shrub, herb and grass strata. This study proves that the extended land incorporated into the Reserve contributes to the biological diversity of the Reserve. / Environmental Sciences / M. Tech. (Nature Conservation)

A population survey was conducted on Galago moholi along the road system at Loskop Dam Nature Reserve, Mpumalanga province, South Africa. The data were analyzed using the maximum perpendicular distance, mean perpendicular distance, and maximum reliable perpendicular distance methods. Vegetation sample plots were constructed at the location of each individual sighted in order to analyze the species’ habitat use. The results indicate that the species density of G. moholi is significantly lower at Loskop Dam Nature Reserve than previously reported at other sites within South Africa. G. moholi was found to prefer areas with high concentrations of Dichrostachys sp., Combretum sp., or Acacia sp. One individual was observed consuming vegetative matter, which may indicate that the population within the reserve is utilizing available resources in a different way than populations in other parts of southern Africa. / Department of Anthropology

As part of a vegetation survey program for the newly acquired farms incorporated into the Loskop Dam Nature Reserve, the vegetation of the Hondekraal Section was investigated. The study provides an ecological basis for establishing an efficient wildlife management plan for the Reserve. From a TWINSPAN classification, refined by Braun-Blanquet procedures, 12 plant communities, which can be grouped into eight major plant communities, were identified. A classification and description of the major plant communities are presented as well as a management plan. Descriptions of the plant communities include characteristic species as well as prominent and less conspicuous species of the tree, shrub, herb and grass strata. This study proves that the extended land incorporated into the Reserve contributes to the biological diversity of the Reserve. / Environmental Sciences / M. Tech. (Nature Conservation)

M.Sc. / Please refer to full text to view abstract

The principal reason for the construction of Loskop Dam was to provide irrigation water to wheat farmers settling in the Olifants River valley in the 1920s. Agriculture has since developed in the area and today, the Loskop Irrigation Board supplies water to > 700 properties with an area of 25 600 ha farming cotton, wheat, citrus and grapes near the town of Groblersdal. Serious concerns were raised about deteriorating water quality when the crocodile population began to decline and the frequency of large fish kills increased from 2006. Crocodile (Crocodylus niloticus) and Mozambique tilapia (Oreochromis mossambicus) mortalities were linked to pansteatitis which is characterised by obesity and lipid peroxidation. Known impacts on water quality include eutrophication and acid mine drainage from coal mining with associated increases of soluble metals. The aims of this study were to: i) determine whether pansteatitis could be linked to any specific parameters in the water chemistry and limnology of Loskop Dam; ii) measure concentrations of aluminium, copper, iron, manganese, selenium and zinc in various tissues of O. mossambicus to determine whether bio-accumulation was occurring and could be related to pansteatitis; iii) assess the metazoan parasite communities of O. mossambicus to determine whether they are effective indicators of ecosystem health in Loskop Dam. Four established sampling sites were used at Loskop Dam and a reference site was located at neighbouring Kranspoort Dam. Surface water quality samples were collected monthly between July and December 2010 from each site and analysed for 27 constituents including nutrients, major ions, total metals, pH and dissolved oxygen using standard methods. Orthophosphate and total inorganic nitrogen results frequently categorised Loskop Dam as eutrophic and the transitional zone of the dam was characterised by very alkaline conditions resulting from algal blooms (median pH 9.67) which increase the solubility of metals like Al. A combination of active and passive biomonitoring techniques were used for fish collection. Fish gills, brain, muscle, liver and bone were analysed for Al, Fe, Mn, Zn, Cu and Se concentrations. The most striking result was an unanticipated significant deficiency in liver Cu concentrations of fish from Loskop Dam (mean 3.4 mg kg-1) compared to fish from Kranspoort Dam (mean 62 mg kg-1). Both endo- and ectoparasites were identified and enumerated on the fish and infection rates were calculated as mean intensity (I), mean abundance (A) and prevalence (P). Fish from Loskop Dam had extremely low infection rates and two fish had no parasites whatsoever. The ratio between monoxenous and heteroxenous parasites was calculated and was very high in fish from Loskop Dam compared to fish in Kranspoort Dam and Tompi Seleka, indicating a degraded aquatic ecosystem. While no single factor was outstanding as a possible cause of pansteatitis in this study, these findings provide a good foundation from which to formulate further research questions. Copyright / Dissertation (MSc)--University of Pretoria, 2012. / Paraclinical Sciences / unrestricted

The vegetation of the Nooitgedacht section of the Loskop Dam Nature Reserve resembles Bankenveld vegetation and differs from the other areas of the reserve. This study was undertaken to identify, classify, and describe the plant communities present on this section, and to determine their veld condition. The Braun-Blanquet approach was followed to classify the different plant communities. A total number of 170 sample plots (100m2) were placed in all homogeneous vegetation units in a randomly stratified basis. The Ecological Index Method (EIM) was used to determine the veld condition. Data were collected using the steppoint method and incorporated into the GRAZE model from where the veld condition was calculated. A minimum of 400 step points were surveyed in each community with more points in the larger communities. Plant community data was analysed using the JUICE software program. A total of 11 plant communities were identified. The overall veld condition score indicates the vegetation to be in a good condition, resulting in a high grazing capacity. / Environmental Sciences / M. Sc. (Nature Conservation)

The vegetation of the Nooitgedacht section of the Loskop Dam Nature Reserve resembles Bankenveld vegetation and differs from the other areas of the reserve. This study was undertaken to identify, classify, and describe the plant communities present on this section, and to determine their veld condition. The Braun-Blanquet approach was followed to classify the different plant communities. A total number of 170 sample plots (100m2) were placed in all homogeneous vegetation units in a randomly stratified basis. The Ecological Index Method (EIM) was used to determine the veld condition. Data were collected using the steppoint method and incorporated into the GRAZE model from where the veld condition was calculated. A minimum of 400 step points were surveyed in each community with more points in the larger communities. Plant community data was analysed using the JUICE software program. A total of 11 plant communities were identified. The overall veld condition score indicates the vegetation to be in a good condition, resulting in a high grazing capacity. / Environmental Sciences / M. Sc. (Nature Conservation)

D. Tech. Chemistry / The increase in industrialisation in South Africa has led to an increase in coal mining and coal utilisation in Mpumalanga. This has resulted in the deterioration in the quality of water upstream of Loskop Dam. Little or no information is available on the occurrence or fractionation of trace metals in sediments, which are reservoirs for pollutants. Furthermore, no information is available concerning the occurrence of polycyclic aromatic hydrocarbons (PAHs), known for their carcinogenic and mutagenic effects, in rivers in the area. The possible impact of leaching of elements and organic compounds from South African coals has not been exhaustively researched. The general objectives of the study were to: 1) assess the impact of potentially toxic metals and organic compounds leaching from coal, on the quality of surface waters via simulated leaching experiments; 2) determine the extent of pollution by trace metals and polycyclic aromatic hydrocarbons of the Olifants, Klein Olifants and Wilge rivers, at selected points in the upper catchment area; and 3) identify and apportion sources of polycyclic aromatic hydrocarbons in the sediment samples from the study area.

M.Sc. / The catchment area of the Olifants River has, over a number of years, been exposed to extensive mining, agriculture and urbanisation activities in the Witbank-Middelburg and Phalaborwa regions which has largely contributed to the deterioration of water quality. Of major concern is the influence anthropogenic activities have on the aquatic ecosystem of the Olifants River within the Kruger National Park and how me health of fish residing In these waters is affected. To determine fish health, a biological monitoring method, the Health Assessment Index (H41), was tested for the first time In South Africa. Four surveys were conducted at Mamba and Balule In the Olifants River, Kruger National Park during 1994 (February, May, July and November). An additional survey was conducted in February 1995 at Loskop Dam. Samples of water and sediment were taken for analysis of metals and physical and chemical water parameters. A maximum of 20 Clarias gariepinus fish were sampled at each location. Evaluation of the fish was done according to guidelines set in the HAI and parasite population composition (prevalence, abundance, mean intensity) was determined. Organ and tissue samples including gills, liver, muscle and skin were analysed for the bioaccumulation of chromium, copper, Iron, manganese, nickel, lead, strontium and zinc, using atomic absorption spectrophotomeby. Metal concentrations in the water at Mamba and Balule were within guideline limits, whereas concentrations at Loskop Dam were above guidelines. As reflected by the application of the Aquatic toxicity Index, Loskop Dam presented with the poorest water quality followed by Mamba then Balule. It was found that certain physical and chemical variables namely fluoride, potassium, sulphate and total dissolved solids concentrations at Mamba and Balule were relatively high, particularly during drier months. Metals accumulated in organs and tissues, with the highest concentrations In the gills followed by the liver, skin and muscle. The discriminant analysis, utilising metal bloaccumulation, discriminates between water quality at Mamba and Balule, revealing a 100 % classification probability for each survey. Values obtained In the application of the HAI indicated that variables with good predictor accuracy were plasma protein, all parasites, endoparasites, liver, white blood cell counts, ectoparasites, skin, fins and gills. The discriminant function for the HAI generally Indicated variables similar to those exhibiting high predictor accuracy. The discriminant function showed relatively low classification probability for each survey. In case 1, where separate endo- and ectoparasite variables were Included in the determination of me discriminant function, probability for me entire study ranged between 47.5 % and 84.2 %. In case 2, where endo- and ectoparasite variables were given a refined score rating system, probability ranged between 62.5 % and 100 %. The low classification probability Indicates either the Importance of repetitive testing for this technique or a total departure from it. Results showed that fish populations with higher HAI values are found in water of poorer quality (Mamba), while healthier fish populations i.e. with lower RAI values are found In water of better quality (Balule). Parasite data shows a similar tendency by indicating that ectoparasites are abundant in water of higher quality, while endoparasftes increase in water of poorer quality. The HAI reflects the condition of fish populations in relation to their aquatic environment, therefore, the HA/ gives an indication of water quality and should be used as a first level screening tool. If complemented by a parasite survey, distinguishing between endoand ectoparasites, the results from the HAI will be enhanced.