The variability of the marker TG5 TG gene and its association with the intramuscular fat and marbling of meat in cattle was studied. Randomly selected population, composed of 237 individuals (bulls) of the Czech Pied cattle breed from four farms in the Czech Republic, waschosen for testing polymorphisms. Polymerase chain reaction was used to amplify a fragment of TG 545 bp in size. After amplification, the fragments digestion was performed using the restriction endonuclease BstYI. To verify the presence of the PCR product and to identify the sizes of digested frag-ments the horizontal agarose electrophoresis was performed. The population's absolute and relative frequencies of alleles and genotypes were calculated. The calculations reve-aled a high frequency of the T allele (25.98 %). In conclusion, statisticalanalysis was performed. As a result, the influence of C422T polymorphism in the 5'promoter region of the gene on the TG and on the IMT marblinghave not been proven, but on the contra-ry there was detected significant effect (p<0.05) of polymorphism content on the region of Lauric acid (C12:0). The results also reveal some evidence of polymorphism having influence on Timnodonic acid and meat pH.