Köln, Univ., Diss., 2001. / Computerdatei im Fernzugriff.

Köln, Univ., Diss., 2001. / Computerdatei im Fernzugriff.

Köln, University, Diss., 2001.

Hidrótropos são uma classe de compostos, que em alta concentração, aumentam a solubilidade de uma variedade de compostos hidrofóbicos em água. A principal propriedade dos hidrótropos está relacionada a MHC (concentração hidrotrópica mínima) na qual os hidrótopos começam a agregar. Neste trabalho, uma série de compostos aromáticos que mostram o efeito hidrotrópico foram usados: xileno sulfonato de sódio, salicilato de sódio, p-tolueno sulfonato de sódio, benzoato de sódio e estireno sulfonato de sódio. Diferentes técnicas foram utilizadas para avaliar a MHC, tais como, condutividade, tensão superficial, espalhamento de luz, viscosidade e fluorescência. A espectroscopia de fluorescência foi utilizada para determinar por sonda interna e externa , o grupo fenil do anel dos compostos, é utilizada como uma sonda fluorescente interna. A contribuição da intensidade dos monômeros e excímeros (Iaggr/Imonomol) foram determinados e o pireno foi utilizado como uma sonda externa determinando as intesidades relativas I1/I3. Foi observado que os valores de MHC determinados pelos diferentes métodos são concordantes entre si. O método baseado na intensidade de emissão agregado / monomol, apresentou vantagens quando comparado com outros métodos, pois não requer o uso de sondas ou aditivos, que proporcionariam a desagregação dos hidrótropos. / Hydrotropes are a class of compounds that, at a high concentration, enhance the solubility of a variety of hydrophobic compounds in water. The main property of the hydrotropes is related to the MCH (minimum hydrotropic concentration) at which hydrotropes start to aggregate. In this work, a series of aromatics compounds that showed hydrotropic effects is used: sodium xylenesulfonate, sodium salicylate, sodium p-toluenesulfonate, sodium benzoate and sodium styrenesulfonate. Different techniques were used to evaluate the MHC, like as: conductivity, surface tension, light scattering, viscosity and fluorescence. The fluorescence spectroscopy was used to determine the MHC by external and internal probe; the moiety phenyl of compounds ring served as the internal fluorescence probe. Intensities of the monomer and excimer contributions (Iaggr/Imonomol) were determined and the pyrene was used as external probe measuring the ratio of intensities I1/I3. It is shown that MHC values determined by different methods agree well. The method based on the aggregate to monomol emission ratio showed advantages when compared to the other method, because it doesn\'t require the use of probes or other additives, which might disrupt the aggregation process.

Hidrótropos são uma classe de compostos, que em alta concentração, aumentam a solubilidade de uma variedade de compostos hidrofóbicos em água. A principal propriedade dos hidrótropos está relacionada a MHC (concentração hidrotrópica mínima) na qual os hidrótopos começam a agregar. Neste trabalho, uma série de compostos aromáticos que mostram o efeito hidrotrópico foram usados: xileno sulfonato de sódio, salicilato de sódio, p-tolueno sulfonato de sódio, benzoato de sódio e estireno sulfonato de sódio. Diferentes técnicas foram utilizadas para avaliar a MHC, tais como, condutividade, tensão superficial, espalhamento de luz, viscosidade e fluorescência. A espectroscopia de fluorescência foi utilizada para determinar por sonda interna e externa , o grupo fenil do anel dos compostos, é utilizada como uma sonda fluorescente interna. A contribuição da intensidade dos monômeros e excímeros (Iaggr/Imonomol) foram determinados e o pireno foi utilizado como uma sonda externa determinando as intesidades relativas I1/I3. Foi observado que os valores de MHC determinados pelos diferentes métodos são concordantes entre si. O método baseado na intensidade de emissão agregado / monomol, apresentou vantagens quando comparado com outros métodos, pois não requer o uso de sondas ou aditivos, que proporcionariam a desagregação dos hidrótropos. / Hydrotropes are a class of compounds that, at a high concentration, enhance the solubility of a variety of hydrophobic compounds in water. The main property of the hydrotropes is related to the MCH (minimum hydrotropic concentration) at which hydrotropes start to aggregate. In this work, a series of aromatics compounds that showed hydrotropic effects is used: sodium xylenesulfonate, sodium salicylate, sodium p-toluenesulfonate, sodium benzoate and sodium styrenesulfonate. Different techniques were used to evaluate the MHC, like as: conductivity, surface tension, light scattering, viscosity and fluorescence. The fluorescence spectroscopy was used to determine the MHC by external and internal probe; the moiety phenyl of compounds ring served as the internal fluorescence probe. Intensities of the monomer and excimer contributions (Iaggr/Imonomol) were determined and the pyrene was used as external probe measuring the ratio of intensities I1/I3. It is shown that MHC values determined by different methods agree well. The method based on the aggregate to monomol emission ratio showed advantages when compared to the other method, because it doesn\'t require the use of probes or other additives, which might disrupt the aggregation process.

The major histocompatibility complex (MHC) is a genomic region comprised of a linked cluster of genes and gene families that play an important role in both the adaptive and innate immune responses. Genes within the MHC have also been associated with susceptibility and/or resistance to certain diseases, such as haemochromatosis, insulindependent diabetes, and psoriasis. As a result of these associations the MHC is one the most extensively studied regions of the mammalian genome. The MHCs of a wide variety of species, such as human (HLA), mouse (H-2), pig (SLA), and cow (BoLA), have been characterized with respect to gene content, genomic organization of class I, class II, and class III regions, and comparative organization. Comparative analyses have been useful in delineating the evolutionary development of the MHC. While the MHC of many mammalian species has been investigated, little research has been performed on the equine (Equus caballus) MHC. The equine MHC is referred to as the equine lymphocyte antigen (ELA) complex and is located on chromosome ECA20q. The research that has been done on ELA focused on identifying gene copy number and genetic polymorphisms in the classical class I and class II genes. To better characterize the gene content and organization of ELA, we isolated 103 bacterial artificial chromosome (BAC) clones from a horse BAC library containing well conserved genes found within mammalian MHCs. These BAC clones were assembled into two sequence-ready ordered contigs that span the ELA complex. The first contig which has a minimum tiling path of nine BAC clones contains the ELA class II region and spans 800 kb. The class I and III regions are contained within the second contig which has a 14 BAC clone minimum tiling path and spans 1.6 Mb. This study will report on the construction of the two BAC contigs which span the ELA complex, and characterization of the gene content and organization of the ELA complex.

Thesis (doctoral)--München, 1983.

University, Diss.--Leiden, 2005. / Zsfassung in niederl. Sprache.

Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 4 uppsatser.

Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 5 uppsatser.