No description available.

The gastrocnemius muscle was removed from the hindlimb of female rodents, minced into 1mm3 fragments and autotransplanted. Experimental groups had the sural or tibial nerves laid in the mince as follows: both nerves (BN), sural only (SN), tibial only (TN) or no nerve (NN). Animals were sacrificed 45 days postsurgery and the regenerate and contralateral muscles studied for fiber composition and aerobic capacity. Regenerate mass and aerobic capacity were significantly less than control and followed the order BN > TN > SN > NN. The percentage of Types I, IIA and IIB fibers were also different between treatment groups. The data suggests (1) either a more rapid development of axons associated with IIA fibers following nerve transection or greater reinnervation capacity, and/or (2) a shift in the relative number of these axons in the regenerating nerve.

This series of experiments was designed to examine various relationships between physical training and acid-base regulation in blood and muscle of man. The specific purposes of these studies were to 1) determine if the buffer capacity of blood differs between endurance trained and untrained males, 2) examine the effects of eight weeks of sprint training on the buffer capacity of blood and muscle, and 3) determine if a difference exists in the buffer capacity of blood and muscle between endurance trained and sprint trained males. Buffer capacity of blood, measured with an exercise titration of blood, was found to be not different between untrained and either sprint trained or endurance trained subjects. The buffer capacity of muscle, measured by observing the change in muscle pH created by known changes in muscle lactate, was found to be significantly greater in sprint trained subjects than both untrained and endurance trained subjects. Muscle buffer capacity was not different between untrained and endurance trained subjects. It was concluded that buffer capacity is atrainable entity but that its improvement is localized within muscle and is specific to the type of training employed.

No description available.

Ion exchange chromatography methods which have been developed in recent years appear to offer a sensitive technique that can be utilized very advantageously in studies on various proteins and their properties. The application of such a procedure for the successful fractionation of bovine sarcoplasmic proteins should stimulate interest and research in characterizing the changes occurring in beef muscle during the post-mortem aging period. The research described herein pertains to the development and application of a DEAE-cellulose ion exchange chromatography procedure for the fractionation of bovine sarcoplasmic proteins. Results of preliminary experiments indicated that columns packed under pressure possessed superior fractionational qualities than did columns packed without pressure. Also in the preliminary experiments, a Tris buffer system (a starting buffer of 0.04 M Tris phosphate, pH 9.0, and a limiting buffer of 0.5 M Tris H₂PO₄, pH 3.6) and a concave gradient elution procedure were developed which were found to separate the sarcoplasmic proteins satisfactorily. At least 16 components were recognized to be fractionated in the chromatography of beef sarcoplasmic proteins extracted two hours post-mortem. Duplication of the chromatographic results was found to be quite good. Some changes that had occurred in the sarcoplasmic proteins during a post-mortem aging period of 10 days were detected by the chromatographic technique. The changes observed were the appearance of new components, and disappearance of some fractions while others diminished. Data obtained from experiments on the pre-chromatographic treatment of the samples showed that any deviation in procedure always resulted in chromatographic differences. Hence, strict uniformity must be maintained throughout the chromatographic procedure in order to obtain reproducible results. Although the experimental evidence obtained in this study indicates that further research must be completed on the chromatographic procedure, the method does offer a sensitive technique for gaining new information on the properties of the sarcoplasmic proteins. / Graduation date: 1965

In the skeletal muscle cell membrane, the voltage gated chloride channel, CIC-1, maintains as unusually high resting membrane conductance and thereby prevents myotonic skeletal muscle disease. Protein crystallization experiments with bacterial CIC proteins, provide the information for the three dimensional (3D) structure of CIC chloride channels. / Thesis (PhDBiomedicalScience)--University of South Australia, 2003.

Thesis (MAppSc in Physiotherapy)--University of South Australia, 1997

Thesis (Ph. D.)--Victoria University (Melbourne, Vic.), 2006. / Includes bibliographical references.

Thesis (M.S.)--Oregon State University, 2008. / Printout. Includes bibliographical references. Also available on the World Wide Web.

Thesis (Ph.D.)--McMaster University, 2002. / Adviser: Chris M. Wood. Includes bibliographical references (leaves 165-177). Also available via World Wide Web.