This thesis describes the design and implementation of a control system for testing line using ultrasonic testing and magnetic particle testing. The test line is designed for non-destructive testing of railway wheels with detection and localization of surface and subsurface defects. The thesis describes the design and operating principles of the test line. On this basis, the design of a control system that controls various processes throughout the test line is clearly described. The proposed control system was implemented in the industrial application.

The theoretical part summarizes information on the isolation and purification of plasmid DNA and nucleic acids as. Plasmid DNA is often used in gene engineering as a vector for the transfer of a particular gene. Its insulation and transportation in sufficient quality is crucial for other processes associated with it. Isolation and survival of pDNA using magnetic carriers of different concentrations of PEG 8000 in combination with 1M NaCl was investigated in experimental parts. Furthermore, the isolation of pDNA using commercial kits was examined.

This diploma thesis resolves aplication of particular non-destructive testing methods while producing castings of steel wheels and quality control of this process. There are described fundamentals of flaw detection, determination of flaw-generation cause and suggestions for optimalization of casting process and increasing of its quality.

No description available.