Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-06-13T19:40:46Z No. of bitstreams: 1 EloiseCristinaDeSouzaRodrigues_TESE.pdf: 3994565 bytes, checksum: b9c31f2d2181715131a7f58b458b5b2a (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-06-16T20:54:23Z (GMT) No. of bitstreams: 1 EloiseCristinaDeSouzaRodrigues_TESE.pdf: 3994565 bytes, checksum: b9c31f2d2181715131a7f58b458b5b2a (MD5) / Made available in DSpace on 2017-06-16T20:54:23Z (GMT). No. of bitstreams: 1 EloiseCristinaDeSouzaRodrigues_TESE.pdf: 3994565 bytes, checksum: b9c31f2d2181715131a7f58b458b5b2a (MD5) Previous issue date: 2017-02-03 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Nanopart?culas de ?xido de Fe t?m sido muito usadas em aplica??es biom?dicas devido a sua biocompatibilidade e biodegradabilidade. Dentre dessas aplica??es destacamos a hipertermia magn?tica para o tratamento de diversos tipos de c?nceres. Neste trabalho usamos a moagem de alta energia para a produ??o de nanopart?culas de magnetita com tamanho inferior a 70 nm. Preparamos dois grupos de amostras, o primeiro grupo apresentou as fases de magnetita e ?-Fe, composto pelas amostras S1 e S3, o segundo grupo apresentou as fases de magnetita, ?-Fe e w?stita, amostras S2 e S4. Para melhorar a dispers?o dos materiais em meio aquoso, revestimos as nanopart?culas com o surfactante ?cido oleico e o pol?mero Pluronic-F127. As propriedades estruturais e qu?micas das amostras foram analisadas atrav?s da difra??o de raios X, microscopia eletr?nica de transmiss?o e espectroscopia M?ssbauer. As propriedades magn?ticas foram estudadas atrav?s de medidas de susceptibilidade AC e de medidas de magnetiza??o em fun??o do campo e da temperatura. Das medidas M?ssbauer, para a amostra S4, observamos uma raz?o entre as ?reas dos espectros referentes aos s?tios octaedrais e tetraedrais compat?veis com a magnetita estequiom?trica. As medidas magn?ticas mostraram a transi??o de Verwey da magnetita em 120 K para os dois grupos de amostras. Atrav?s das imagens de microscopia eletr?nica, de S4, verificamos que a w?stita encontra-se depositada sobre as nanopart?culas de magnetita. O nosso estudo mostra que a presen?a da w?stita e a funcionaliza??o com ?cido oleico evitaram a oxida??o do Fe2+ presente na magnetita. O deslocamento das curvas de histerese, nas medidas feitas com a amostra resfriada em presen?a de campo magn?tico, ? atribu?do ? intera??o de troca que ocorre na interface entre a w?stita e a magnetita. As medidas de susceptibilidade AC mostraram picos caracter?sticos de paredes de dom?nio, para a amostra com duas fases, indicando que uma parcela das part?culas ? multidom?nio. Quando as nanopart?culas funcionalizadas e n?o funcionalizadas foram submetidas a um campo magn?tico alternado apresentaram uma varia??o de temperatura e 11? C e 53? C, para S3P e S1, respectivamente, e para S4 a varia??o de temperatura foi de 14? C. Os resultados indicam que as amostras t?m potencial para uso em hipertermia magn?tica. / Oxide nanoparticles have been used in several biomedical applications due to their biocompatibility and biodegradability. Among these applications, magnetic hyperthermia of tumors has been proposed as an alternative treatment of several neoplastic diseases. In this work, we have used high energy milling to produce iron oxide nanoparticles with sizes smaller than 70 nm. We have prepared two groups of samples, the first group is composed of magnetite and ?-Fe, samples S1 and S3, the second group is composed of magnetite, w?stite and ?-Fe nanoparticles, samples S2 and S4. To improve the sample?s dispersibility in aqueous medium, the samples were functionalized with oleic acid and Pluronic-F127 three-block copolymer. The structural and chemical properties of samples were studied through x-ray diffraction, transmission electron microscopy (TEM) and M?ssbauer spectroscopy. The magnetic properties were studied through AC susceptibility and DC magnetization as a function of temperature and field. From the M?ssbauer studies, for the sample S4, we observed a ratio between the areas of the spectra for the octahedral and tetrahedral compatible with stoichiometry magnetite. The magnetic measurements showed the Verwey transition at about 120 K, for the two groups of samples. From the TEM images, S4, we verified that the w?stite phase is formed on the magnetite nanoparticles. Therefore, we showed that the w?stite phase and oleic acid surfactant prevent the magnetite nanoparticles from further oxidation. The shift of hystereis loops observed in the field cooled samples is ascribed to exchange interaction between the w?stite and magnetite phases. The AC susceptibility, from S4, showed characteristic peaks of magnetite wall domains, this result indicated that a fraction of particles are multidomain. The samples were submitted to an AC magnetic field and we observed an increase in temperature of e 11? C and 53? C for samples functionalized and bare, for S1 and S4 respectively, for sample S4, the increase in temperature, was of 14? C. The combination of magnetic properties, the ability to release heat in presence of an AC, and the stability of particles in aqueous suspension suggest that these samples are good candidates for magnetic hyperthermia.