Spelling suggestions: "subject:"aintenance off pluripotent"" "subject:"aintenance oof pluripotent""
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Simultaneous binding of bFGF to both FGFR and integrin maintains properties of primed human induced pluripotent stem cells / bFGFがFGFRとインテグリンに同時に結合することがプライム型ヒト人工多能性幹細胞の未分化状態を制御する鄭, 羽伸 23 May 2024 (has links)
京都大学 / 新制・課程博士 / 博士(医学) / 甲第25490号 / 医博第5090号 / 新制||医||1073(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 長船 健二, 教授 江藤 浩之, 教授 斎藤 通紀 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM
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The Screening of Biomaterials to Support Long-term Growth and Maintenance of Human Embryonic Stem Cells in Xeno- and Feeder-free SystemPang, Justin Tse Wei 09 December 2013 (has links)
Current feeder-free culture systems employing undefined Matrigel are still more effective in maintaining human embryonic stem (ES) cells than defined surfaces using extracellular matrix (ECM) proteins. While the role of substrate stiffness in stem cell fate is becoming increasingly evident, all previous culture systems use ECM proteins on rigid polystyrene surfaces. Here, we used factorial designs to screen and evaluate combinations ECM proteins and substrate stiffness for their effect on short-term pluripotency and self-renewal. Using optimal conditions determined from our screening experiments, defined and near xeno-free culture systems maintained CA1 human ES cells for over 10 passages in Essential 8 (E8) medium. Under these conditions, we found that human ES cell self-renewal was greater on soft polydimethylsiloxane (PDMS) substrates than on rigid polystyrene dishes. The culture systems and screening tools developed in this project will help develop robust and defined xeno-free culture systems that incorporate both biochemical and biomechanical factors.
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The Screening of Biomaterials to Support Long-term Growth and Maintenance of Human Embryonic Stem Cells in Xeno- and Feeder-free SystemPang, Justin Tse Wei 09 December 2013 (has links)
Current feeder-free culture systems employing undefined Matrigel are still more effective in maintaining human embryonic stem (ES) cells than defined surfaces using extracellular matrix (ECM) proteins. While the role of substrate stiffness in stem cell fate is becoming increasingly evident, all previous culture systems use ECM proteins on rigid polystyrene surfaces. Here, we used factorial designs to screen and evaluate combinations ECM proteins and substrate stiffness for their effect on short-term pluripotency and self-renewal. Using optimal conditions determined from our screening experiments, defined and near xeno-free culture systems maintained CA1 human ES cells for over 10 passages in Essential 8 (E8) medium. Under these conditions, we found that human ES cell self-renewal was greater on soft polydimethylsiloxane (PDMS) substrates than on rigid polystyrene dishes. The culture systems and screening tools developed in this project will help develop robust and defined xeno-free culture systems that incorporate both biochemical and biomechanical factors.
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