Thesis (Msc Food Sc (Food Science))--University of Stellenbosch, 2009. / The use of near infrared (NIR) hyperspectral imaging and hyperspectral image analysis for distinguishing between whole maize kernels of varying degrees of hardness and fungal infected and non-infected kernels have been investigated. Near infrared hyperspectral images of whole maize kernels of varying degrees of hardness were acquired using a Spectral Dimensions MatrixNIR camera with a spectral range of 960-1662 nm as well as a sisuChema SWIR (short wave infrared) hyperspectral pushbroom imaging system with a spectral range of 1000-2498 nm. Exploratory principal component analysis (PCA) on absorbance images was used to remove background, bad pixels and shading. On the cleaned images, PCA could be used effectively to find histological classes including glassy (hard) and floury (soft) endosperm. PCA illustrated a distinct difference between floury and glassy endosperm along principal component (PC) three. Interpreting the PC loading line plots important absorbance peaks responsible for the variation were 1215, 1395 and 1450 nm, associated with starch and moisture for both MatrixNIR images (12 and 24 kernels). The loading line plots for the sisuChema (24 kernels) illustrated peaks of importance at the aforementioned wavelengths as well as 1695, 1900 and 1940 nm, also associated with starch and moisture. Partial least squares-discriminant analysis (PLS-DA) was applied as a means to predict whether the different endosperm types observed, were glassy or floury. For the MatrixNIR image (12 kernels), the PLS-DA model exhibited a classification rate of up to 99% for the discrimination of both floury and glassy endosperm. The PLS-DA model for the second MatrixNIR image (24 kernels) yielded a classification rate of 82% for the discrimination of glassy and 73% for floury endosperm. The sisuChema image (24 kernels) yielded a classification rate of 95% for the discrimination of floury and 92% for glassy endosperm. The fungal infected and sound whole maize kernels were imaged using the same instruments. Background, bad pixels and shading were removed by applying PCA on absorbance images. On the cleaned images, PCA could be used effectively to find the infected regions, pedicle as well as non-infected regions. A distinct difference between infected and sound kernels was illustrated along PC1. Interpreting the PC loading line plots showed important absorbance peaks responsible for the variation and predominantly associated with starch and moisture: 1215, 1450, 1480, 1690, 1940 and 2136 nm for both MatrixNIR images (15 and 21 kernels). The MatrixNIR image (15 kernels) exhibited a PLS-DA classification rate of up to 96.1% for the discrimination of infected kernels and the sisuChema had a classification rate of 99% for the same region of interest. The The iv sisuChema image (21-kernels) had a classification rate for infected kernels of 97.6% without pre-processing, 97.7% with multiplicative scatter correction (MSC) and 97.4% with standard normal variate (SNV). Near infrared hyperspectral imaging is a promising technique, capable of distinguishing between maize kernels of varying hardness and between fungal infected and sound kernels. While there are still limitations with hardware and software, these results provide the platform which would greatly assist with the determination of maize kernel hardness in breeding programmes without having to destroy the kernel. Further, NIR hyperspectral imaging could serve as an objective, rapid tool for identification of fungal infected kernels.

Thesis (PhD)--Stellenbosch University, 2013. / ENGLISH ABSTRACT: Maize (Zea mays L.) is used for human and animal consumption in diverse forms, from specialised foods in developed countries, to staple food in developing countries. Unfortunately, maize is prone to infection by different Fusarium species that can produce harmful mycotoxins. Fusarium verticillioides is capable of asymptomatic infection, where infected kernels show no sign of fungal growth, but are contaminated with mycotoxins. If fungal contamination is not detected early on, mycotoxins can enter the food chain. Rapid and accurate methods are required to detect, identify and distinguish between pathogens to enable swift decisions regarding the fate of a batch or consignment of cereal. Near infrared (NIR) hyperspectral imaging and multivariate image analysis (MIA) were evaluated to investigate the fungal development in maize kernels over time. When plotting principal component (PC) 4 against PC5, with percentages sum of squares (%SS) 0.49% and 0.34%, three distinct clusters were apparent in the score plot and this was associated with degree of infection. Prominent peaks at 1900 nm and 2136 nm confirmed that the source of variation was due to changes in starch and protein. Variable importance plots (VIP) confirmed the peaks observed in the PCA loading line plots. Early detection of fungal contamination and activity (20 h after inoculation) was possible before visual symptoms of infection appeared. Using NIR hyperspectral imaging and MIA it was possible to differentiate between species of Fusarium associated with maize. It was additionally applied to examine the fungal growth kinetics on culture media. Partial least squares discriminant analysis (PLS-DA) prediction results showed that it was possible to discriminate between species, with F. verticillioides the least correctly predicted (between 16-47% pixels correctly predicted). For F. subglutinans 78-100% and for F. proliferatum 60-80% pixels were correctly predicted. Three prominent bands at 1166, 1380 and 1918 nm were considered to be responsible for the differences between the growth zones. Variations in the bands at 1166 and 1380 nm were correlated with the depletion of carbohydrates as the fungus grew while the band at 1918 nm was a possible indication of spore and new mycelial formation. By plotting the pixels from the individual growth zones as a function of time, it was possible to visualise the emergence and interaction of the growth zones as separate growth profiles. The microstructure of fungal infected maize kernels was studied over time using high resolution X-ray micro-computed tomography (μCT). The presence of voids and airspaces could be seen in two dimensional (2D) X-ray transmission images and in the three dimensional (3D) tomograms. Clear differences were detected between kernels imaged after 20 and 596 h of inoculation. This difference in voids as the fungus progressed showed the effect of fungal damage on the microstructure of the maize kernels. Imaging techniques are important for rapid, accurate and objective evaluation of products for quality and safety. NIR hyperspectral imaging offers rapid chemical evaluation of samples in 2D images while μCT offers 3D microstructural information. By combining these image techniques more value was added and this led to a comprehensive evaluation of Fusarium infection in maize. / AFRIKAANSE OPSOMMING: Mielies (Zea mays L.) word in verskeie vorms deur mens en dier verbruik, van gespesialiseerde voedsel in ontwikkelde lande, tot stapelvoedsel in ontwikkelende lande. Ongelukkig is mielies onderhewig aan besmetting deur verskeie Fusarium spesies wat skadelike mikotoksiene kan produseer. Fusarium verticilloioides is in staat tot asimptomatiese infeksie waar die besmette pit geen teken van fungusgroei toon nie, maar (reeds) met mikotoksiene besmet is. Indien fungusbesmetting nie vroegtydig opgespoor word nie, kan mikotoksiene die voedselketting betree. Vinnige en akkurate metodes word benodig om patogene op te spoor, te identifiseer en ook om onderskeid tussen patogene te tref om sodoende (effektiewe) besluite aangaande die gebruik van ‘n lot of besending graan te neem. Naby-infrarooi (NIR) hiperspektrale beelding en meerveranderlike beeld ontleding (MIA) is geëvalueer om fungusontwikkeling in mieliepitte oor tyd te ondersoek. Wanneer hoofkomponent (PC) 4 teenoor PC5 gestip word, met persentasies som van kwadrate (%SS) 0.49% en 0/34%, is drie afsonderlike groepein die telling grafiek waargeneem. Dit is geassosieer met die graad van besmetting. Prominente pieke by 1900 nm en 2136 nm het bevestig dat veranderinge in stysel en proteïene die bron van die variasie was. Veranderlike belangrikheidsgrafieke (VIP) het die pieke wat in die PCA beladingslyngrafieke waargeneem is, bevestig. Vroegtydige opsporing (bespeuring) van fungusbesmetting en aktiwiteit (20 h na inokulasie) was moontlik voor visuele besmettingsimptome verskyn het. Onderskeid tussen Fusarium spesies wat met mielies geassosieer word, was moontlik deur gebruik te maak van NIR hiperspektrale beelding en MIA. Dit is bykomend toegepas om fungusgroeikinetika op kwekingsmedia te bestudeer. Parsiële kleinste kwadrate diskriminantanalise (PLS-DA) voorspellingsresultate het getoon dat dit moontlik was om tussen spesies te onderskei, met F. verticillioides die minste korrek voorspel (tussen 19-47% beeldelemente korrek voorspel). Vir F. subglutinans is 78-100% en vir F. proliferatum is 60-80% beeldelemente korrek voorspel. Drie prominente bande by 1166, 1380 en 1918 nm is oorweeg as oorsaak vir die verskille tussen die groeisones. Variasies in die bande by 1166 en 1380 nm is gekorreleer met die vermindering van koolhidrate soos die fungus groei, terwyl die band by 1918 nm ‘n moontlike aanduiding van spoor en nuwe miseliale vorming is. Deur die beeldelemente van die individuele groeisones as ‘n funksie van tyd te stip, was dit moontlik om die verskyning en interaksie van die groeisones as aparte groeiprofiele te visualiseer. Hoë-resolusie X-straal mikro-berekende tomografie (μCT) is gebruik om die mikrostruktuur van fungusbesmette mieliepitte oor tyd te ondersoek. Die voorkoms van leemtes en lugruimtes kon in die twee-dimensionele (2D) X-straal transmissie beelde en in die drie-dimensionele (3D) tomogramme gesien word. Duidelike verskille is waargeneem tussen pitte wat na 20 en 596 h na inokulasie verbeeld is. Hierdie verskil in leemtes soos die fungus vorder, het die effek van fungusskade op die mikrostruktuur van mieliepitte getoon. Beeldingstegnieke is belangrik vir vinnige, akkurate en objektiewe evaluasie van produkte vir kwaliteit en veiligheid. NIR hiperspektrale beelding bied vinnige chemiese evaluering van monsters in 2D beelde, terwyl μCT 3D mikrostrukturele inligting gee. Meer waarde is toegevoeg deur hierdie beeldingstegnieke te kombineer en dit het gelei tot ‘n omvangryke evaluering van Fusarium besmetting in mielies.

The importance of maize in sub-Saharan Africa and the potential of quality protein maize (QPM) to alleviate the nutritional gap caused by lack of access to adequate protein rich foods were highlighted. Frustrations from complex inheritance systems of the QPM trait leading to calls for more information on the inheritance and stability of the QPM trait, fear of total loss of the QPM trait due to the recessive nature of the opaque-2 gene to the wild type gene in normal endosperm maize when QPM and normal endosperm maize coexist, lack of information on the nutritional value of contaminated QPM grain, and poor linkages with the smallholder farmers were all cited as drawbacks in the promotion and adoption of QPM. Therefore the objectives of the study were: 1) To solicit the participation of smallholder farmers in the development and setting up of QPM breeding goals, objectives and dissemination strategies; 2) To estimate general combining ability (GCA), specific combining ability (SCA) and reciprocal cross effects on anthesis days, quality traits and grain yield among the publicly available elite QPM inbred lines; 3) To compare experimental QPM hybrids with selected check cultivars, and normal endosperm maize hybrids for grain yield performance and kernel endosperm modification scores; 4) To evaluate QPM hybrids for grain yield and kernel endosperm modification scores in selected sub-Saharan Africa target environments. 5) To determine the level of normal endosperm maize pollen contamination that can occur in quality protein maize without loss of nutritional superiority; 6) To estimate the average levels and the patterns of foreign maize pollen contamination in QPM crops coexisting with normal endosperm maize varieties. The contribution of smallholder farmers in setting breeding goals and dissemination strategies for QPM was solicited. One major finding was that the kernel endosperm qualities of landrace “Hickory King” need to be incorporated into new QPM varieties so as to encourage adoption. Farmers preferred getting information on QPM varieties through their local Agricultural Research and Extension (AREX) officers. A diallel study of 36 F1 QPM hybrids and their reciprocals was conducted across seven environments for agronomic traits and three environments for nutritional value traits. There were significant differences for all traits analysed using Griffing Method 3 model 1. General combing ability effects were significant and important in the control of anthesis days, kernel endosperm modification, protein content, tryptophan content, and Quality Index (QI). Specific combining ability effects were highly significant and important in the control of grain yield. There were significant SCA effects for anthesis days and QI but the proportions were lesser than the corresponding GCA effects in both traits. Kernel endosperm modification had significant GCA effects and nonsignificant SCA effects. Reciprocal-cross differences were significant for anthesis days, tryptophan content and QI. Nonmaternal effects were significant for tryptophan content whilst both maternal and nonmaternal effects were significant for QI and anthesis days. Nonmaternal effects were relatively more important than maternal effects in all the cases where there were significant reciprocal-cross differences. The cross with the highest SCA effects for grain yield was CZL03016/CML144. The most desirable cross with the lowest anthesis days was CZL03016/CML144 whilst the most desirable inbred line with the lowest anthesis GCA effects was CZL03016. The inbred line with the most desirable GCA effects for protein content, tryptophan content and QI was CML264Q. Inbred line CML264Q crossed to CZL03016 had significant SCA effects for QI. The most desirable GCA effects for kernel endosperm modification were associated with inbred line CZL03016 followed by CZL01006. Maternal effects for both tryptophan content and QI were associated with inbred line CML264Q. Genotype by environment interaction effects across all the seven environments were significant for grain yield and kernel endosperm modification. Check hybrids performed better than experimental hybrids for grain yield but were not different for kernel endosperm modification. The normal endosperm maize hybrids were significantly better for both grain yield and kernel endosperm modification. However, in all the comparisons the best check or normal endosperm maize hybrid was not significantly better than the best experimental or QPM hybrid, respectively. The most desirable score for kernel endosperm modification was from the cross of CZL01006 to CZL03016 though not significantly different from the check hybrid with the best score. AMMI1 was the best model for kernel endosperm modification scores and AMMI2 was suitable for grain yield. Both environments and hybrids were diverse. Grain yield of most hybrids was not stable with specific adaptation to environments. The most stable hybrid with no specific adaptation was CML176/CML181f with a mean yield of 6.51t ha-1. The putative nutritional superiority of normal endosperm maize pollen contaminated QPM as measured by the QI depended on the environmental conditions. The moisture stressed environment (CIMMYT Harare) had a lower QI value (0.858) and a lower tolerance to pollen contamination of 15.3% whereas the grain produced under near to optimum growing conditions (ART farm) had a higher QI value (0.915) and a higher tolerance to pollen contamination of 31.9% before total loss of nutritional superiority. Thus contaminated QPM grain had nutritional superiority up to a certain point before total loss of nutritional superiority. Geostatistical analysis was used to determine the levels and patterns of pollen contamination that occur when QPM and normal endosperm maize crops coexist under conditions minimising both temporal and geographical isolation to the lowest possible levels for the two independent crops. Higher pollen contamination levels were restricted mostly to the sections of the QPM crop proximal to the rows of normal endosperm maize crop, with the central parts of the QPM crops experiencing relatively low levels of contamination. For the four experiments (QCS200711, QCS200712, QCS200721 and QCS200722) in which the thresholds to nutritional superiority were determined, 87.9%, 94.8%, 62.2% and 65.6% of the crop areas passed for superior QPM grain, respectively. Estimates for average contamination levels of homogenous mixtures of grain from each of the nine experiments were below 20% contamination. The contamination levels were far less than previously thought. “Hickory King” kernel quality attributes were important in breeding QPM varieties for the smallholder farmers. Parents of the 72 hybrids were diverse for the agronomic characters studied and three of the experimental hybrids were found to be adapted and comparable to the check varieties. Quality protein maize tolerance to foreign pollen contamination without loss in nutritional superiority depended on growing conditions. The coexistence of QPM and normal endosperm maize without total loss of QPM nutritional superiority was feasible. / Thesis (PhD.)-University of KwaZulu-Natal, Pietermaritzburg, 2008.

Vitamin A deficiency (VAD) is a major public health problem in developing countries, including South Africa. The potential of provitamin A-biofortified maize for use as a complementary strategy to alleviate vitamin A deficiency in developing countries, where maize is the dominant staple food, is currently a subject of research. Although the nutritional composition of white maize is thought to be similar to that of biofortified maize, apart from the differences in provitamin A carotenoid content, the comparative nutritional composition of the two maize types seems not to have been subjected to a comprehensive scientific study. When setting the target level of provitamin A in the provitamin A-biofortified maize, it is important to consider the potential effect of processing on the final provitamin A carotenoid content of the biofortified food products, as the provitamin A carotenoids levels may decrease on processing. Furthermore, the yellow/orange provitamin A-biofortified maize may not be widely accepted by African consumers who are vulnerable to VAD, and are traditional consumers of white maize. This study firstly aimed to evaluate the nutritional composition, including provitamin A composition, and grain quality of provitamin A-biofortified maize varieties, compared to white maize. The second aim was to assess the effect of processing (milling and cooking) on the retention of provitamin A carotenoids and other nutrients in popular South African maize food products prepared with provitamin A-biofortified maize. Thirdly, the study aimed to assess the acceptability of maize food products prepared with provitamin A-biofortified maize by consumers of different age and gender in rural KwaZulu-Natal, South Africa. The grains of the provitamin A-biofortified maize varieties and grain of a white maize variety (control) were analysed for their nutritional composition using standard or referenced methods. The carotenoid content of the grains was analysed by High-Performance Liquid Chromatography (HPLC) and mass spectroscopy. The provitamin A carotenoids β-cryptoxanthin, and trans and cis isomers of β-carotene, and other unidentified cis isomers of β-carotene were detected in varying levels in the provitamin A-biofortified maize varieties. The total provitamin A content in the biofortified maize varieties ranged from 7.3-8.3 μg/g dry weight (DW), with total β-carotene ranging from 3.5-3.6 μg/g DW, and β-cryptoxanthin from 3.7-4.8 μg/g DW, whilst no carotenoids were detected in the white maize variety. Results of the evaluation of the content of other nutrients showed that, when compared with the white maize variety, the provitamin A-biofortified maize varieties had higher levels of starch, fat and protein but were lower in iron. The zinc and phosphorus levels in the white maize and the biofortified maize were comparable. The biofortified maize varieties were better sources of most of the essential amino acids relative to the white maize, but, similar to the white maize, they were deficient in histidine and lysine, indicating that further improvement is required. Selected quality attributes (grain density, susceptibility of kernels to cracking, milling quality and resistance of the kernels to fungal infection) of grains of 32 provitamin A-biofortified maize varieties and a white variety (control) were assessed. Overall, the quality of the grains of the provitamin A-biofortified maize varieties were found to be superior to that of the white maize grain, although the biofortified maize grains showed less resistance to fungi, including mycotoxin-producing types. This indicates that the trait of grain resistance to infection by fungi should also be incorporated in the provitamin A-biofortified maize varieties during breeding. To assess the retention of provitamin A carotenoids and other nutrients in maize food products, three selected provitamin A-biofortified maize varieties and the control (white maize variety) were milled into mealie meal and samp. The milled products were cooked into three products: phutu and thin porridge (from the mealie meal) and cooked samp. Nutrient retention during processing was determined. Milling resulted in either an increase or slight decrease in the provitamin A carotenoid levels, but there was no major decrease in the total provitamin A level. Most of the other nutrients were well retained during milling, but there were substantial losses of fibre, fat and minerals. Provitamin A carotenoid levels decreased on cooking. In phutu 96.6 ± 20.3% β-cryptoxanthin and 95.5 ± 13.6% of the β-carotene was retained after cooking. In thin porridge 65.8 ± 4.6% β-cryptoxanthin and 74.7 ± 3.0% β-carotene; and in samp 91.9 ± 12.0% β-cryptoxanthin and 100.1 ± 8.8% of the β-carotene was retained after cooking, respectively. Provitamin A retention seemed to be influenced by both maize variety and food form, indicating that suitable varieties and food forms should be found. There was generally a high retention of the other nutrients in all the three cooked products, except for the substantial losses of fat in thin porridge and iron and phosphorus in cooked samp. These findings indicate that an optimal delivery of provitamin A to the consumer can be achieved by processing provitamin A-biofortified maize into foods that have a good retention of provitamin A carotenoids, such as phutu and samp. These food products would be recommended in areas where VAD is prevalent. In order to assess consumer acceptability of provitamin A-biofortified maize, a total of 212 subjects aged 3-55 years from Mkhambathini Municipality, in KwaZulu-Natal province, South Africa, participated in the sensory evaluation of phutu, thin porridge and cooked samp prepared with provitamin A-biofortified maize varieties and a white variety (control). Preference for yellow maize food products was negatively associated with an increase in the age of the subjects. Overall, preschool children preferred yellow maize to white maize food products: phutu (81% vs. 19%), thin porridge (75% vs. 25%) and samp (73% vs. 27%). In contrast, primary school children preferred white maize to yellow maize food products: phutu (55% vs. 45%), thin porridge (63% vs. 38%) and samp (52% vs. 48%). Similarly, secondary school children and adults also displayed a similar preference for white maize food products. There was no association between gender and preference for maize variety. Focus group discussions revealed that participants had a negative attitude towards biofortified maize due to its colour, taste, smell and texture. However, the participants expressed a willingness to consume biofortified maize if it was cheaper than white maize and was readily available in local grocery stores. These findings indicate that there is a potential to promote the consumption of provitamin A-biofortified maize and its food products in this part of South Africa, thereby contributing to a reduction in the incidence of VAD. This study has shown that provitamin A-biofortified maize has a good potential to be used as an additional strategy to alleviate VAD in poor communities of South Africa, including similar environments in sub-Saharan Africa. However, the study has revealed that there are still challenges to be overcome in order to achieve the target provitamin A content of 15 μg/g in provitamin A-biofortified maize, set by HarvestPlus, an international challenge program. This may also explain why provitamin A-biofortified maize varieties with this level of provitamin A have been scarcely reported in the literature. Thus, more research is required to achieve the target provitamin A level in maize by conventional breeding. The results of this study indicate that besides provitamin A, the biofortified maize is also a good source of other nutrients including starch, fat, protein and zinc. However, improving the consumer acceptability of the provitamin A-biofortified maize remains a challenge, due to the negative attitudes towards the yellow/orange maize by African consumers. On the other hand, the results of this study indicate that there is an opportunity to promote the consumption of provitamin A-biofortified maize food products by preschool children, a finding which has not been previously reported in the literature. Nutrition education on the benefits of provitamin A-biofortified maize, as well as improved marketing are recommended, in this part of South Africa and also in similar environments in other sub-Saharan countries. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2011.

A sample of 260 farm households that were randomly selected in Katumba ward, Rungwe district, Tanzania were studied for the effects of storage methods on the quality of maize grain and household food security using qualitative and quantitative methods. Maize storage problems, amounts of maize that farm households harvested and amounts of maize that farm households lost to pests per year, food security status and farm households’ perceptions concerning their food security status were investigated using face - to - face semi - structured and structured interviews. Common storage methods that farm households used to store maize and the dietary importance of maize were investigated through interviews, seasonal calendars and the matrix for scoring and ranking. The quality of maize was investigated through conducting mycological analysis and through investigating levels of insect infestation using the incubation method on maize samples collected from a sub-sample of 130 farm households at harvest and after five months of storage period. It was found that farm households in Katumba ward preferred maize meal rather than other types of food that provide bulk such as rice and green bananas/plantains. Maize contributed 66.8 % - 69.5 % of the total energy and 83 - 90 % of the total protein required per day, and farm households stored maize using roof and sack storage methods. It was also found that 34.5 % of 2323 tonnes of maize that were harvested per annum in Katumba ward were lost to pests during storage. Fusarium, Diplodia, Aspergillus and Penicilliums species were identified as the main fungal pathogens that attacked stored maize. Sitophilus zeamais, Sitotroga cerealella and rodents were also identified as the main maize storage pests. About 25 % of the maize samples that were collected at harvest and 93 % of the maize samples that were collected from the same farm households after five months of storage were infested by either Sitophilus zeamais or Sitotroga cerealella or both. Maize samples from the two storage systems had an average number of 80 insect pests per 120 maize kernels (or 51 g of maize), amounting to 1569 insects per kg. The high levels of insect infestation reduced the amount of maize that could have been available to the farm households and subjected stored maize to fungal infections and subsequent contaminations, thus, rendering the farm households vulnerable to food insecurity. Furthermore, it was also found that most of the infestation of maize by insect pests and moulds in Katumba ward occurred during storage, and that farm households were not well informed concerning maize storage and the negative effects that fungal activities in maize can have on the health of the consumers. An average of 87717 μg/kg fumonisins, 596 μg/kg aflatoxins, 745 μg/kg ochratoxins and 1803 μg/kg T-2 toxins were detected in the maize samples. Currently, there are no set standards for T-2 toxins, whereas the internationally accepted standards for aflatoxins, fumonisins and ochratoxins in cereals are 20 μg/kg, 4 mg/kg and 50 μg/kg, respectively. It was concluded that the levels of mycotoxins detected in maize from Katumba ward were far above the internationally accepted standards and that the farm households were at risk of ill health through consuming maize meals made from contaminated maize grain. The presence of high concentrations of mycotoxins, together with the high levels of insect infestation in the maize led to the conclusion that reduction of the nutrient content of the maize grain in Katumba ward was inevitable. Thus, the pests that infested maize stored using the roof and sack storage methods in this ward compromised not only the availability of food, but also the utilization of the nutrients in the maize and its safety, leading to the farm households’ food insecurity. It was further concluded that the quality of maize stored using roof and sack storage methods in Katumba ward was low and that the roof and sack storage methods were inadequate for protecting stored maize from pests. It was recommended that an efficient method for rapid drying of maize prior to storage be found, that the roof and sack storage methods be improved so that they can effectively protect stored maize from moisture content problems. It was also recommended that the farm households’ awareness concerning maize storage and food security be raised, and that the extension staff in Katumba ward should urge the Tanzanian government to implement an agricultural policy which promotes efficient maize storage and maize quality in order to improve the current status quo. Above all, since maize is the predominant staple, it was recommended that the maize breeding program in Tanzania should emphasize development of maize varieties that are resistant to ear rots, storage insects and to contamination by mycotoxins as part of a larger program to improve food security in this part of the country. Breeding programs that aim at enhancing the nutritional value of maize were also recommended. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2011.