Motion capture has in recent years grown in interest in many fields from both game industry to sport analysis. The need of reflective markers and expensive multi-camera systems limits the business since they are costly and time-consuming. One solution to this could be a deep neural network trained to extract 3D joint estimations from a 2D video captured with a smartphone. This master thesis project has investigated the accuracy of a trained convolutional neural network, MargiPose, that estimates 25 joint positions in 3D from a 2D video, against a gold standard, multi-camera Vicon-system. The project has also investigated if the data from the deep neural network can be connected to a biomechanical modelling software, AnyBody, for further analysis. The final intention of this project was to analyze how accurate such a combination could be in golf swing analysis. The accuracy of the deep neural network has been evaluated with three parameters: marker position, angular velocity and kinetic energy for different segments of the human body. MargiPose delivers results with high accuracy (Mean Per Joint Position Error (MPJPE) = 1.52 cm) for a simpler movement but for a more advanced motion such as a golf swing, MargiPose achieves less accuracy in marker distance (MPJPE = 3.47 cm). The mean difference in angular velocity shows that MargiPose has difficulties following segments that are occluded or has a greater motion, such as the wrists in a golf swing where they both move fast and are occluded by other body segments. The conclusion of this research is that it is possible to connect data from a trained CNN with a biomechanical modelling software. The accuracy of the network is highly dependent on the intention of the data. For the purpose of golf swing analysis, this could be a great and cost-effective solution which could enable motion analysis for professionals but also for interested beginners. MargiPose shows a high accuracy when evaluating simple movements. However, when using it with the intention of analyzing a golf swing in i biomechanical modelling software, the outcome might be beyond the bounds of reliable results.