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Thesis (MAppSc) -- AUT University, 2009. / Includes bibliographical references. Also held in print (xv, 305 leaves : ill. ; 30 cm.) in City Campus Theses Collection (T 639.809932 WON)

Thesis (M.S.)--Ohio University, August, 2003. / Title from PDF t.p. Includes bibliographical references (leaves 67-75)

Thesis (PhD)--Stellenbosch University, 2013. / ENGLISH ABSTRACT: Historical vicariant processes due to glaciations, resulting from the large-scale environmental changes during the Pleistocene (0.012-2.6 million years ago, Mya), have had significant impacts on the geographic distribution of species, especially also in marine systems. The motivation for this study was to provide novel information that would enhance ongoing efforts to understand the patterns of biodiversity on the South African coast and to infer the abiotic processes that played a role in shaping the evolution of taxa confined to this region. The principal objective of this study was to explore the effect of Pleistocene climate changes on South Africa′s marine biodiversity using five intertidal gastropods (comprising four rocky shore species Turbo sarmaticus, Oxystele sinensis, Oxystele tigrina, Oxystele variegata, and one sandy shore species Bullia rhodostoma) as indicator species. Sequence data obtained from partial segments of the mitochondrial cytochrome oxidase c subunit 1 (COI), and the nuclear ribosomal DNA (encompassing part of 5.8S, second Internal Transcribed Spacer and part of 28S, hereinafter called ITS2; or comprising part of the first Internal Transcribed Spacer, 5.8S, second Internal Transcribed Spacer and part of 28S, hereinafter called ITS), were used as genetic markers to construct phylogeographic patterns and to investigate demographic histories of the taxa. Population structure was investigated using haplotype network analyses, pairwise ΦST statistics, analysis of molecular variance (AMOVA), isolation by distance analyses, Bayesian analysis of population structure (BAPS) and coalescent analysis of gene flow. Demographic history was analysed through Fu′s Fs tests, mismatch distributions, and Bayesian skyline plots. Demographic analyses suggest that all five intertidal gastropods studied experienced demographic expansions dating to the late Pleistocene. The sandy shore direct developer B. rhodostoma began expansion after the LGM (c. 15 kya) whereas for the four rocky shore broadcast spawners (T. sarmaticus, O. sinensis, O. tigrina, and O. variegata) the onset of expansion coincided with or preceded the LGM (c. 25, 60, 50, 40 kya, respectively). Consistent with recent range expansions and gene flow patterns, the population genetic structure in all species was characterised by shallow or a lack of population differentiation. Oxystele variegata was an exception as it showed a deep disjunction, of late Pleistocene origin, between individuals in the west coast Namaqua Bioregion and those in the south coast Agulhas Bioregion. These results provide strong evidence of the vital role that Pleistocene climatic changes and current regimes played in shaping the nature and distribution of biodiversity on the South African coast. In addition, gene flow in all species, except O. tigrina, was remarkably asymmetrical with the regions around Cape Infanta and Port Elizabeth acting as source populations. Considering the generally weak population genetic structure and gene flow patterns detected for most gastropod species studied here, it is recommended that T. sarmaticus, O. sinensis, O. tigrina and B. rhodostoma be managed as panmictic populations, and that the region encompassing Cape Infanta, and Port Elizabeth should be prioritised for conservation as it appears to harbour source populations. Oxystele variegata was the only species showing distinct population structure and in this instance, species specific conservation efforts should recognize this divergence by treating the two genetic assemblages as distinct management units. / AFRIKAANSE OPSOMMING: Historiese vikariante prosesse kan toegeskryf word aan glasiasie, en het tot gevolg gehad dat grootskaalse veranderinge in die omgewing plaasgevind het tydens die Pleistoseen (,012 - 2.6 miljoen jaar gelede, Mjg). Dit het 'n beduidende impak gehad op die geografiese verspreiding van spesies, veral ook in die mariene stelsels. Die motivering vir hierdie studie was om nuwe data te voorsien wat sal bydrae tot die voortgesette pogings om die patrone van biodiversiteit langs die Suid-Afrikaanse kus te verstaan. Dit sou ook help om die abiotiese prosesse af te lei wat 'n rol gespeel het in die evolusie van taksa wat in hierdie streek voorkom. Die hoofdoel van hierdie studie was om die effek van die Pleistoseen klimaatsveranderinge op Suid-Afrika se mariene biodiversiteit te bepaal deur gebruik te maak van vyf intergety slak spesies as indikatore (vier wat in rotsagtige gebiede voorkom: Turbo sarmaticus, Oxystele sinensis, Oxystele tigrina, Oxystele variegata en 'n sanderige strand spesies: Bullia rhodostoma). Volgorde data verkry vanaf gedeeltelike segmente van die mitochondriale sitochroom oksidase c subeenheid 1 (COI), en die kern ribosomale DNA (bestaande uit 'n deel van 5.8S, tweede interne getranskribeerde spasieërders en 'n deel van 28S), hierna genoem ITS2 is gebruik as genetiese merkers om filogeografiese patrone te dokumenteer en ook om die demografiese geskiedenis van die spesies te ondersoek. Bevolking struktuur is ondersoek deur gebruik te maak van haplotipe netwerk analise, paarsgewyse ΦST statistiek, analise van molekulêre variansie (AMOVA), isolasie deur afstand analise, Bayesiaanse analise van die bevolking struktuur (BAPS) en analise van gene vloei. Demografiese geskiedenis is ontleed deur Fu se Fs toetse, misparing verdelings, en Bayesiaanse luglyn kurwes. Demografiese ontleding dui daarop dat al vyf die intergety slakke wat ondersoek is demografiese uitbreidings ervaar het wat terugdateer tot die einde van die Pleistoseen. Die sanderige strand direkte ontwikkelaar, B. rhodostoma, het die uitbreiding begin na die LGM (c. 15 Kya), terwyl vir die vier rotsagtige kusbewoners wat eiers oor 'n uitgebreide gebiede versprei (T. sarmaticus, O. sinensis, O. tigrina, en O. variegata) het die aanvang van die bevolkings uitbreiding saamgeval met die laaste galsiasie of dit voorafgegaan (c. 25, 60, 50, 40 Kya, onderskeidelik). In ooreenstemming met die onlangse reeks bevolkings uitbreidings, is die bevolking genetiese struktuur in alle spesies gekenmerk deur weinig differensiasie. Oxystele variegata was 'n uitsondering en het 'n ontwrigting van laat Pleistoceen oorsprong getoon tussen individue langs die weskus Namaqua Biostreek en dié in die suid kus Agulhas biostreek. Hierdie resultate voorsien sterk bewyse van die belangrike rol wat die Pleistoseen klimaatsveranderinge gespeel het in die vorming en verspreiding van biodiversiteit langs die Suid-Afrikaanse kus. Daarbenewens, geen vloei in alle spesies, behalwe O. tigrina, was merkwaardig asimmetries. Kaap Infanta en Port Elizabeth verteenwoordig moontlik die bron bevolkings. Met inagneming van die geringe bevolking genetiese struktuur en geenvloei patrone wat waargeneem is vir die meeste slak spesies wat bestudeer is, word dit aanbeveel dat T. sarmaticus, O. sinensis, O. tigrina en B. rhodostoma bestuur word as 'n panmiktiese bevolking, en dat die streek wat Kaap Infanta en Port Elizabeth insluit geprioritiseer moet word vir bewaring. Oxystele variegata was die enigste spesie wat duidelike bevolking struktuur getoon het en in hierdie geval, moet spesie spesifieke bewaringspogings aangewend word.

Thesis (M.S.) in Marine Policy--University of Maine, 2009. / Includes vita. Includes bibliographical references (leaves 170-181).

Although international law requires States to protect the marine environment and conserve marine living resources, human activities are still threatening marine species and its survival. In view of limited scientific knowledge of marine ecosystems, fragility of ecosystems and insufficient mechanisms of protection, marine biodiversity in areas beyond national jurisdiction is at risk. Increasing attention has been given to the need to conserve this important and vulnerable biodiversity. The international community has begun to recognize the importance of marine protected areas (MPAs) as key tool to ensure sustainable use and preservation of biodiversity. However, the adequacy of the current legal framework related to the conservation of marine biodiversity through the establishing of high seas MPAs raises debates. Disagreements about the existence of inadequacies and need for an implementing agreement remain. My study seeks to determine whether the international legal framework is adequate for the establishment of MPAs or whether further measures are required.

Although international law requires States to protect the marine environment and conserve marine living resources, human activities are still threatening marine species and its survival. In view of limited scientific knowledge of marine ecosystems, fragility of ecosystems and insufficient mechanisms of protection, marine biodiversity in areas beyond national jurisdiction is at risk. Increasing attention has been given to the need to conserve this important and vulnerable biodiversity. The international community has begun to recognize the importance of marine protected areas (MPAs) as key tool to ensure sustainable use and preservation of biodiversity. However, the adequacy of the current legal framework related to the conservation of marine biodiversity through the establishing of high seas MPAs raises debates. Disagreements about the existence of inadequacies and need for an implementing agreement remain. My study seeks to determine whether the international legal framework is adequate for the establishment of MPAs or whether further measures are required.

Thesis (Ph. D.)--University of British Columbia, 1999. / Includes bibliographical references (leaves 146-164).

The role of natural environments, especially ‘green space’, in promoting human health and well-being is well-researched. However, less is known about the benefits of ‘blue space’ (e.g. the coast) or ‘managed’ nature (e.g. zoos). In this thesis, six studies investigated the restorative potential of sub-aquatic settings, specifically public aquariums and the biological diversity within them. Studies 1 and 2 investigated preferences for, affective responses to, and the restorative potential of, five built and natural settings (i.e. built, green space, blue space, natural underwater and public aquariums). Using the same measures, Study 3 focused solely on people’s responses to aquarium exhibits, sub-categorised by geographic region, level of species richness and abundance, and taxonomic group. Study 4 examined behavioural, physiological and psychological responses to one aquarium exhibit during three stages of restocking. Finally, to establish how perceptions of species richness and abundance influenced well-being outcomes, people viewed and evaluated either one (Study 5) or two (Study 6) large aquarium exhibits. These studies found that natural sub-aquatic and aquarium settings were as preferred, and perceived as potentially restorative, as green space environments, and different exhibits elicited different responses: ratings were higher for tropical exhibits (vs. temperate), high abundance (vs. low) and vertebrates (vs. invertebrates), although findings for species richness were mixed. Within one exhibit, higher stocking levels resulted in increased attention and interest, greater improvements in mood, and some physiological evidence of relaxation (e.g. decreased heart rate). Broad levels of marine life could be distinguished but estimates of actual numbers were poor. Viewing one or two exhibits tended to improve mood, decrease arousal and be perceived as restorative; any differences between the two exhibits were more evident when both had been viewed. Overall, findings suggest that engaging with different types of managed nature may provide valuable perceived human health and well-being benefits.

An estimated 500 million cases of malaria occur each year. The increasing prevalence of drug resistant strains of Plasmodium in most malaria endemic areas has significantly reduced the efficacy of current antimalarial drugs for prophylaxis and treatment of this disease. Therefore, discovery of new, inexpensive, and effective drugs are urgently needed to combat this disease. Marine biodiversity is an enormous source of novel chemical entities and has been barely investigated for antimalarial drug discovery. In an effort to discover novel therapeutics for malaria, we studied the antimalarial activities of a unique marine-derived peak fraction library provided by Harbor Branch Oceanographic Institute (HBOI). Within this unique library, we have screened 2,830 marine natural product (MNP) peak fractions through a medium throughput screening effort utilizing the SYBR Green-I fluorescence based assay, and have identified 253 fractions that exhibit antimalarial activity. From those inhibiting fractions we have identified twenty species of marine organisms that inhibit Plasmodium falciparum growth, from which thirty-five fractions were selected for further study. Among those thirty-five, eighty-three percent were also found to inhibit the chloroquine resistant strain of P. falciparum, Dd2. The most potent inhibitors were then screened for their cytotoxic properties using the MTT cell viability assay. Among the samples that exhibited potent inhibition of P. falciparum growth were fractions derived from a sponge of the genus Spongosorites sp.. This genus of sponge has been reported to contain the nortopsentin and topsentin class of bis-indole imidazole alkaloids. Nortopsentin A inhibited the parasite growth at the trophozoite stage with an IC50 value of 1.6 µM. This is the first report of antimalarial activity for this class of compound.