Spelling suggestions: "subject:"matrixassisted laser desorption"" "subject:"aeroassisted laser desorption""
1 |
Characterisation and ionisation modelling of matrices in MALDI mass spectrometryAllwood, Daniel Anthony January 1998 (has links)
No description available.
|
2 |
Microfluidic electrocapture technology in protein and peptide analysis /Astorga-Wells, Juan, January 2004 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst., 2004. / Härtill 5 uppsatser.
|
3 |
Simulation studies of the ion cooling processes of MALDI derived ions in fourier-transform mass spectrometry.January 2006 (has links)
Ko Ka Lung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2006. / Includes bibliographical references. / Abstracts in English and Chinese. / Title page --- p.i / Abstract (English) --- p.ii / Abstract (Chinese) --- p.iii / Acknowledgement --- p.iv / Declaration --- p.v / Table of Content --- p.vi / List of Figure --- p.viii / Chapter 1. --- INTRODUCTION --- p.1 / Chapter 1.1 --- Matrix-assisted Laser Desorption / Ionization (MALDI) --- p.2 / Chapter 1.1.1 --- Evolution of Matrix-assisted laser desorption / ionization (MALDI) --- p.2 / Chapter 1.1.1.1 --- Lasers --- p.3 / Chapter 1.1.1.2 --- Matrices --- p.3 / Chapter 1.1.1.3 --- Sample preparation --- p.4 / Chapter 1.1.1.4 --- Desorption --- p.6 / Chapter 1.1.1.5 --- Ionization --- p.7 / Chapter 1.2 --- Fourier Transform Ion Cyclotron Resonance Mass Spectrometry with MALDI (FTICR-MS) --- p.9 / Chapter 1.2.1 --- History of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry --- p.9 / Chapter 1.2.2 --- Basics of FTICR-MS --- p.11 / Chapter 1.2.3 --- FTICR couple with external ionization source --- p.15 / Chapter 1.2.4 --- Coupling of MALDI to FTICR --- p.16 / Chapter 1.3 --- Problems encountered on the coupling of MALDI to FTICR-MS --- p.17 / Chapter 1.4 --- Outline of present work --- p.19 / Chapter 2 --- SIMULATION METHOD --- p.20 / Chapter 2.1 --- Overview of the ion optics simulation --- p.21 / Chapter 2.2 --- History of SIMION Program --- p.22 / Chapter 2.3 --- Basics and theory of SIMION version 6.0 --- p.24 / Chapter 2.4 --- Simulation method --- p.26 / Chapter 2.4.1 --- Creating potential array --- p.27 / Chapter 2.4.2 --- User program --- p.29 / Chapter 2.4.3 --- Ion definition parameter --- p.31 / Chapter 2.4.4 --- Trajectories quality panel --- p.33 / Chapter 2.4.5 --- Data recording --- p.36 / Chapter 3 --- OPTIMIZATION OF RF-ONLY HEXAPOLE UNDER PULSE GAS CONDITION --- p.37 / Chapter 3.1 --- Introduction --- p.38 / Chapter 3.2 --- Simulation conditions --- p.39 / Chapter 3.3 --- Results and discussion --- p.40 / Chapter 3.3.1 --- rf-frequency (w) --- p.41 / Chapter 3.3.2 --- rf voltage (Vo-p) --- p.44 / Chapter 3.3.3 --- Pulse gas pressure(po) --- p.47 / Chapter 3.3.4 --- Trapping potential (VT) --- p.49 / Chapter 3.3.5 --- Effect of space charge --- p.53 / Chapter 3.4 --- Conclusions --- p.60 / Chapter 4 --- OPTIMIZATION OF DIFFERENT HEXAPOLE-BASED INTERFACES FOR PRE-TRAPPING COOLING --- p.61 / Chapter 4.1 --- Introduction --- p.62 / Chapter 4.2 --- Simulation conditions --- p.63 / Chapter 4.3 --- Results and discussion --- p.66 / Chapter 4.3.1 --- Static medium pressure interface --- p.66 / Chapter 4.3.1.1 --- Effect of pressure --- p.66 / Chapter 4.3.1.2 --- Effect of space charge --- p.70 / Chapter 4.3.2 --- Differential pressure model (Skimmer-based) --- p.73 / Chapter 4.3.2.1 --- Effect of pressure --- p.73 / Chapter 4.3.2.2 --- Effect of space charge --- p.76 / Chapter 4.3.3 --- A comparison of the optimal operating conditions for the three proposed interfaces --- p.81 / Chapter 4.3.4 --- Comparison of the theoretical results amd the experimental results --- p.83 / Chapter 4.4 --- Conclusion --- p.84 / Chapter 5 --- CONCLUSIONS --- p.85 / Chapter 5.1 --- Conclusions --- p.86 / REFERENCES --- p.R1 / APPENDIX --- p.A1
|
4 |
MALD/I TOF PSD and CID : understanding precision, resolution, and mass accuracy and MALD/I TOFMS : investigation of discrimination issues related to solubility /Hoteling, Andrew J. January 2004 (has links)
Thesis (Ph. D.)--Drexel University, 2004. / Includes abstract and vita. Includes bibliographical references.
|
5 |
Biotyping Saccharomyces cerevisiae strains using matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS)Moothoo-Padayachie, Anushka. January 2011 (has links)
In clinical diagnosis and fermentation industries there is a need for a method that allows for the
differentiation of yeast to the strain level (biotyping). The ideal biotyping method should be
accurate, simple, rapid and cost-effective, and capable of testing a large number of yeast
isolates. Matrix assisted laser desorption/ionization time of flight mass spectrometry has
emerged as a powerful biotyping tool for the identification of bacteria and clinical yeast isolates,
mainly Candida. It has been found that the MALDI-TOF MS signals from yeast are harder to
obtain than from bacteria. It has been reported by several research studies that a cell lysis step
is required to obtain a mass spectral signal for clinical Candida strains. To date an optimized
sample preparation protocol has not been devised for the biotyping of S. cerevisiae strains.
Studies on the identification of yeast using MALDI-TOF MS have focused primarily on clinical
Candida yeast isolates but have included very few S. cerevisiae strains. Furthermore these
yeast identification studies using MALDI-TOF MS have only achieved identification to the
species and not strain level. A major limiting attribute of MALDI-TOF MS for the accurate
identification of microbes, is its dependency on a comprehensive mass spectral database.
Bruker Daltonics is a pioneer and leader in providing innovative life science tools based on
mass spectrometry thus the Bruker Daltonics mass spectral database and state-of-the-art
instruments and accompanying software were selected for this study. The Bruker Daltonics
mass spectral database currently holds three thousand seven hundred and forty
microorganisms of which only a mere seven are S. cerevisiae strains.
Initially in this study, a number of parameters of a generic ethanol/formic acid protein
extraction procedure as originally described by Bruker Daltoincs were considered in the
development of a sample preparation protocol that yielded characteristic and highly
reproducible MALDI-TOF mass spectra. The parameters considered included cell number,
alcohol fixation, matrix solution and media. It was found that using the optimized sample
preparation protocol unique and highly reproducible mass spectral profiles were obtained for all
three S. cerevisiae strains. Multivariate analysis confirmed that the differences between all three
S. cerevisiae strains were statistically significant. For quality assurance, the spectra of the three
strains were sent for evaluation by Bruker Daltonics and were deemed suitable for the purpose
of biotyping.
The newly created ethanol/formic acid extraction procedure was used to generate an S.
cerevisiae mass spectral database comprising of forty-five S. cerevisiae strains within a local
context but also of global significance. The accuracy of the mass spectral database was
assessed using blind coded S. cerevisiae strains obtained from the Agricultural Research
Council Infruitec-Nietvoorbij (Institute for Deciduous Fruit, Vines and Wine), Stellenbosch, South
Africa. It was found that S. cerevisiae identification to the species and more importantly strain
level was achievable with relatively good accuracy. To determine the potential application of
MALDI-TOF MS as an accurate method for S. cerevisiae strain identification in industry, blind
coded S. cerevisiae strains were obtained from Natal Cane Products and subjected to MALDITOF
MS analysis. It was found that four of the pure cultures submitted were correctly identified
to the strain level and the three S. cerevisiae strains incorrectly identified may have been
contaminants or the result of incorrect optimization conditions for the fermentation. Thus MALDITOF
MS was shown to be an accurate identification tool, that may also be used to detect
contaminants or incorrect environmental conditions which can result in substantial losses. / Thesis (M.Sc.)-University of KwaZulu-Natal, Westville, 2011.
|
6 |
Detection of Phytophthora species by MALDI-TOF mass spectrometry /Siricord, Cornelia Charito. January 2005 (has links)
Thesis (Ph.D.)--Murdoch University, 2005. / Thesis submitted to the Division of Science and Engineering. Bibliography: leaves 160-177.
|
7 |
Combining laser capture microdissection and MALDI mass spectrometry for tissue protein profiling methodology development and clinical applications /Xu, Baogang Jonathan, January 2005 (has links)
Thesis (Ph. D. in Chemistry)--Vanderbilt University, May 2005. / Title from title screen. Includes bibliographical references.
|
8 |
Immediate observation of matrix assisted laser desorption ionization products in a Fourier transform mass spectrometer /Fiorentino, Michael Armond, January 2000 (has links)
Thesis (Ph. D.)--University of Texas at Austin, 2000. / Vita. Includes bibliographical references. Available also in a digital version from Dissertation Abstracts.
|
9 |
Exploration of the fundamentals of matrix assisted laser desorption/ionization time-of- flight mass spectrometry /Erb, William Joseph. Owens, Kevin G. January 2007 (has links)
Thesis (Ph. D.)--Drexel University, 2007. / Includes abstract and vita. Includes bibliographical references (leaf 200).
|
10 |
Characterization of affinity ligands by MALDI-TOF MS and the preparation of affinity restricted access mediaWa, Chunling. January 1900 (has links)
Thesis (Ph.D.)--University of Nebraska-Lincoln, 2006. / Title from title screen (site viewed Oct. 10, 2007). PDF text: 234 p. : ill. (some col.) UMI publication number: AAT 3259631. Includes bibliographical references. Also available in microfilm and microfiche formats.
|
Page generated in 0.2496 seconds