Late instar larval Alphitobius diaperinus initiated tunneling damage to polystyrene insulation in search of protected pupation sites. Adult females oviposited upon the surface of the styrofoam but early instar larvae left the styrofoam soon after hatching. Within twenty days, large numbers of adults moved into the styrofoam, expanding the tunnels and depreciating the panels' suitability as pupation sites for the late instar larvae. No oviposition occurred within the tunnels. All lifestages of Alphitobius diaperinus were found to be cannibalistic. Although preliminary tests show that adult mealworms will consume house fly late instar larvae and prepupae, no significant predation of the house fly by A. diaperinus could be detected under simulated natural conditions at the population density tested. Wettable powder formulations of permethrin and carbaryl when sprayed upon styrofoam were comparable in residual activity. Residual activity of both compounds was less when applied to unpainted plywood. The emulsifiable concentrate formulation of permethrin proved to be ineffective. Regression slopes from topical application studies reveal that tetrachlorvinphos may be the material of choice against A. diaperinus because there is less chance of the lesser mealworm developing resistance to tetrachlorvinphos than with either permethrin or carbaryl. Tetrachlorvinphos also showed longer residual effectiveness against A. diaperinus as a surface spray on styrofoam and plywood than did permethrin or carbaryl. / Master of Science

The relationship between the types of construction of high rise caged layer houses and insulation damage produced by the lesser mealworm was examined. Polyurethane insulation which had heavy-weight paper glued to its surface and was installed with tape to seal off the seam between the insulation boards had no infestations. Structures with concrete block pit walls had lower insulation infestations than those houses with wooden walls forming the pits. Houses with a support structure set on top of the concrete block pit wall had lower infestations than houses built with the support structure set directly into the earth. The insulation installed nearest the pit was the most severely infested, and infestation intensity was inversely proportional to insulation height above the manure pit. Damage in extruded polystyrene insulation resulted in a substantial loss of volume of material in the corner areas of the insulation panels, and caused a significant reduction in insulating quality. Observations were made on the effect of manure moisture and poultry house construction materials on lesser mealworm dispersal behavior. Larvae and adults preferred manure habitats of 30 and 40% moisture, and dispersal from the manure significantly increased when manure moisture was increased to levels of 50 and 60%. Larvae climbed a significantly greater distance up a vertical wooden surface than up a vertical concrete block surface under field conditions. Results indicate that structures built with wood pit walls are predisposed to infestations, and that fluctuating manure moisture levels in these houses can indirectly contribute to accelerated infestation by driving the larvae from the manure pits into the insulation. Insecticide sprays, plastic films, paint barriers applied to the surface of extruded polystyrene, and different types of insulation were evaluated for lesser mealworm resistance. In a laboratory study, tetrachlorvinphos and pirimiphos-methyl sprayed on extruded polystyrene produced greater than 90% mortality in larval and adult populations up to 71 weeks postapplication. Larvae were unable to penetrate either chlorpyrifos-impregnated or non-insecticidal polyethylene films. Infestation intensity was inversely proportional to insulation cell size. Effective treatments identified under field conditions were permethrin and pirimiphos-methyl sprays, and two formulations of chlorpyrifos-impregnated latex paint. Insulation materials with a cell size of 1.5 mm were resistant to lesser mealworm field populations. / PH. D. / incomplete_metadata

Thesis (M. Sc. Agriculture (Animal Production)) -- University of Limpopo, 2021 / Two experiments were conducted to determine the effect of replacing soya bean meal with yellow mealworm larvae meal (Tenebrio molitor) in a diet on productivity, gut morphology, carcass characteristics and bone morphometrics of Ross 308 broiler chickens aged one to 42 days. In each experiment, a total of 360 Ross 308 broiler chickens were randomly assigned to the five dietary treatments, each treatment having four replications, and 18 chickens per replicate. Five diets were formulated to contain yellow mealworm replacement levels at 0, 25, 50, 75 and 100% to meet the nutrient requirements of Ross 308 broiler chickens. Data was analysed using the General Linear Model procedures of the Statistical Analysis System, Version 9.3.1 software program. Fisher’s least significant difference (LSD) test was applied for mean separation where there were significant differences (P<0.05). A quadratic regression model was used to determine the levels for optimal responses in the variables measured. The first experiment determined the effect of replacing soya bean meal with yellow mealworm larvae meal on productivity and gut morphology of unsexed Ross 308 broiler chickens aged one to 21 days. Replacement of soya bean meal with yellow mealworm meal in a diet had no effect (p > 0.05) on feed intake, growth rate, FCR, live body weight, ME intake and nitrogen retention of unsexed Ross 308 broiler chickens aged one to 21 days. Replacing soya bean meal with yellow mealworm meal in a diet did not affect (p > 0.05) caecum weight of unsexed Ross 308 broiler chickens. However, replacing soya bean meal with yellow mealworm meal in a diet increased (p < 0.05) gastro intestinal tract, crop, ileum and large intestine weights. Crop and ileum lengths of unsexed Ross 308 broiler chickens aged 21 days were not affected (p > 0.05) by replacement of soya bean meal with yellow mealworm meal in the diet. However, replacing soya bean meal with yellow mealworm meal in a diet increased (p < 0.05) gizzard, caecum and large intestine lengths of unsexed Ross 308 broiler chickens aged 21 days. Yellow mealworm meal in a diet did not affect (p > 0.05) gut organ digesta pH values of unsexed Ross 308 broiler chickens aged 21 days. The second experiment determined the effect of replacing soya bean meal with yellow mealworm meal in a diet on productivity, gut morphology, carcass characteristics and bone morphometrics of Ross 308 broiler chickens aged 22 to 42 days. Replacement of soya bean meal with yellow mealworm meal in a diet did not affect (p > 0.05) growth rate, FCR, ME intake and nitrogen retention of male Ross 308 broiler chickens aged 22 to 42 days. However, replacing soya bean meal with yellow mealworm meal in a diet affected (p < 0.05) feed intake and live body weight of male Ross 308 broiler chickens aged 22 to 42 days. Broiler chickens on diets containing 75 or 100% yellow mealworm meal had higher (p < 0.05) intakes than those on diets containing no yellow mealworm meal. Similarly, male broiler chickens on diets having 50% yellow mealworm meal had higher (p < 0.05) live body weights than those on diets containing no yellow mealworm. Quadratic equations indicated that feed intake and live body weight of male Ross 308 broiler chickens were optimized at yellow mealworm meal replacement levels of 13 and 61%, respectively. The present study showed that replacing soya bean meal with yellow mealworm meal in a diet did not affect (p > 0.05) gut organ digesta pH values, gut organ weights, gut organ lengths, meat colour, meat pH values, bone morphometric values, carcass part weights and meat sensory attributes of male Ross 308 broiler chickens aged 42 days. However, meat from chickens on diets containing yellow mealworm meal was softer (p < 0.05) than meat from chickens on diets having 100% soya bean meal. It is concluded that soya bean meal can be replaced with yellow mealworm larvae meal in a diet at 25, 50, 75 and 100% levels without having adverse effects on production and carcass characteristics of Ross 308 broiler chickens aged one to 42 days