published_or_final_version / Psychiatry / Master / Master of Research in Medicine

published_or_final_version / Community Medicine / Master / Master of Public Health

published_or_final_version / Public Health / Master / Master of Public Health

published_or_final_version / Medical Sciences / Master / Master of Medical Sciences

by Suk-Fung Tsang. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 101-112). / Abstract --- p.i / Acknowledgement --- p.iii / Abbreviation --- p.iv / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- TNF molecule / Chapter 1.2 --- Molecular biosynthesis of TNF / Chapter 1.3 --- Antitumor activity of TNF / Chapter 1.4 --- Macrophage-mediated immunity / Chapter 1.5 --- Endogenous production of TNF / Chapter 1.6 --- LPS : the potent inducer for TNF release / Chapter 1.7 --- Natural product: as primer or inducer / Chapter 1.8 --- Aim of this project / Chapter Chapter 2 --- Materials and Methods --- p.22 / Chapter 2.1 --- Materials / Chapter 2.2 --- Animals / Chapter 2.3 --- Cell line / Chapter 2.4 --- Transformed cell line : EAT cells invivo / Chapter 2.5 --- Reagents / Chapter 2.6 --- Methods / Chapter Chapter 3 --- Preparation of sample --- p.33 / Chapter 3.1 --- Alcohol precipitaion of Bupleuri radix / Chapter 3.2 --- Endogenous TNF production by BR fractions / Chapter Chapter 4 --- Purification of BRI --- p.38 / Chapter 4.1 --- Gel filtration chromatography of BRI / Chapter 4.2 --- Anion exchange chromatography / Chapter Chapter 5 --- Purification of PQI --- p.52 / Chapter 5.1 --- Gel filtration chromatography of PQI / Chapter 5.2 --- Anion exchange chromatography / Chapter Chapter 6 --- Capacity of BR and PQ as eliciting agent for endogenous TNF production --- p.62 / Chapter 6.1 --- Time course of endogenous TNF production by BRI subfractions / Chapter 6.2 --- Time course of endogenous TNF production by PQI subfractions / Chapter 6.3 --- BRI subfractions as eliciting agents / Chapter 6.4 --- PQI subfractions as eliciting agents / Chapter Chapter 7 --- Are BR and PQ priming agents in endogenous TNF production ？ --- p.71 / Chapter 7.1 --- Priming by intraperitoneal route / Chapter 7.2 --- Priming by intravenous route / Chapter Chapter 8 --- Removal of LPS by acetic acid treatment --- p.79 / Chapter Chapter 9 --- Antitumor activities of BRI subfractionsin relationship with TNF production --- p.86 / Chapter 9.1 --- BRI subfraction as eliciting agent / Chapter 9.2 --- Pretreatment with BRIA subfractions followed by LPS treatment / Chapter Chapter 10 --- Conclusion --- p.95 / Bibliography --- p.101

by Yuen-Kam Tsoi. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 160-173). / Abstracts in English and Chinese. / Abstract --- p.i / 論文摘要 --- p.iv / List of figures --- p.vii / List of tables --- p.x / Abbreviations --- p.xi / Chapter Chapter 1 --- Purification and characterization of the chymotrypsin inhibitor (MCCI) from the seeds of Momordica cochinchinensis / Chapter 1.1 --- Introduction l / Chapter 1.1.1 --- Classification of protease inhibitor --- p.2 / Chapter 1.1.2 --- Therapeutic potential of protease inhibitors --- p.4 / Chapter 1.2 --- Rationale of the present study --- p.6 / Chapter 1.3 --- Materials and methods / Chapter 1.3.1 --- Materials --- p.9 / Chapter 1.3.2 --- Preparation of chymotrypsin-Sepharose 4B affinity column --- p.10 / Chapter 1.3.3 --- Protein extraction --- p.11 / Chapter 1.3.4 --- Chymotrypsin-Sepharose 4B affinity chromatography --- p.12 / Chapter 1.3.5 --- Reversed phase high pressure liquid chromatography --- p.12 / Chapter 1.3.6 --- Assays for protease inhibitory activities --- p.14 / Chapter 1.3.6.1 --- Assay for chymotrypsin activity --- p.15 / Chapter 1.3.6.2 --- Assay for trypsin activity --- p.15 / Chapter 1.3.6.3 --- Assay for elastase activity --- p.16 / Chapter 1.3.6.4 --- Assay for subtilisin activity --- p.16 / Chapter 1.3.7 --- Determination of protein concentration --- p.17 / Chapter 1.3.8 --- Titration of chymotrypsin --- p.17 / Chapter 1.3.9 --- Sodium dodecyl sulfate polyacrylamide gel electrophoresis --- p.18 / Chapter 1.3.10 --- Determination of molecular weight by mass spectrometry --- p.19 / Chapter 1.3.11 --- Partial amino acid sequencing --- p.20 / Chapter 1.3.12 --- Effects of chymotrypsin on MCCI --- p.20 / Chapter 1.3.13 --- Stability assay --- p.21 / Chapter 1.4 --- Results --- p.21 / Chapter 1.4.1 --- Isolation of MCCI from the seeds of Momordica cochinchinensis --- p.21 / Chapter 1.4.2 --- N-terminal amino acid sequencing --- p.27 / Chapter 1.4.3 --- Determination of molecular weight --- p.31 / Chapter 1.4.4 --- Inhibitory activity of MCCI towards different proteases --- p.33 / Chapter 1.4.5 --- Effects of chymotrypsin on MCCI --- p.37 / Chapter 1.4.6 --- Stability of MCCI on heating and at different pH --- p.37 / Chapter 1.5 --- Discussion --- p.42 / Chapter Chapter 2 --- Immunomodulatory effect of MCCI / Chapter 2.1 --- Introduction of the immune system and protease inhibitors --- p.51 / Chapter 2.2 --- Rationale of the present study --- p.55 / Chapter 2.3 --- Materials and methods --- p.56 / Chapter 2.3.1 --- Materials --- p.56 / Chapter 2.3.2 --- Isolation of different types of immune cells --- p.57 / Chapter 2.3.3 --- Determination of cell proliferation --- p.60 / Chapter 2.3.4 --- Determination of H2O2 formation --- p.61 / Chapter 2.3.5 --- Assay of interleukin-2 --- p.61 / Chapter 2.3.6 --- Determination of cell viability --- p.62 / Chapter 2.4 --- Results --- p.63 / Chapter 2.4.1 --- Murine splenocytes --- p.63 / Chapter 2.4.1.1 --- In vitro effect of MCCI on the proliferation of murine splenocytes --- p.63 / Chapter 2.4.1.2 --- Effect of MCCI on cytokine production --- p.63 / Chapter 2.4.2 --- Murine lymphocytes --- p.66 / Chapter 2.4.2.1 --- In vitro effect of MCCI on the proliferation of lymphocytes --- p.66 / Chapter 2.4.2.2 --- Effect of MCCI on cytokine production --- p.66 / Chapter 2.4.3 --- Murine bone marrow cells --- p.69 / Chapter 2.4.3.1 --- Effect of MCCI on the growth of murine bone marrow cells --- p.69 / Chapter 2.4.4 --- Murine neutrophills --- p.69 / Chapter 2.4.4.1 --- Effect of MCCI on H2O2 formation --- p.69 / Chapter 2.4.5 --- Murine macrophages --- p.71 / Chapter 2.4.5.1 --- Effect of MCCI on the growth of macrophages --- p.71 / Chapter 2.4.5.2 --- Effect of external ATP on the growth of macrophages --- p.71 / Chapter 2.4.5.3 --- Effect of ATP on the growth of macrophages pre-treated with MCCI --- p.76 / Chapter 2.4.5.4 --- Effect of MCCI on the growth of macrophages pre-treated with ATP --- p.76 / Chapter 2.4.5.5 --- Effect of MCCI on H201production --- p.79 / Chapter 2.5 --- Discussion --- p.82 / Chapter Chapter 3 --- Anti-oxidative effect of MCCI in primary rat hepatocytes culture / Chapter 3.1 --- Introduction --- p.91 / Chapter 3.1.1 --- Liver disease and protease inhibitors --- p.91 / Chapter 3.1.2 --- Primary rat hepatocyte as a pharmacological model --- p.93 / Chapter 3.1.3 --- tert-Butyl hydroperoxide as an oxidative stress inducer --- p.94 / Chapter 3.1.4 --- Endogenous antioxidant enzymes against ROS --- p.96 / Chapter 3.2 --- Rationale of the present study --- p.99 / Chapter 3.3 --- Materials and methods --- p.101 / Chapter 3.3.1 --- Materials --- p.101 / Chapter 3.3.2 --- Isolation of primary rat hepatocytes --- p.101 / Chapter 3.3.2.1 --- Liver perfusion --- p.101 / Chapter 3.3.2.2 --- Preparation of collagen pre-coated culture plates --- p.103 / Chapter 3.3.2.3 --- Hepatocytes culturing --- p.103 / Chapter 3.3.3 --- Drug treatment and oxidative stress induction --- p.104 / Chapter 3.3.4 --- Cytotoxicity assessment --- p.105 / Chapter 3.3.5 --- Cellular GSH content determination --- p.105 / Chapter 3.3.6 --- Protein determination by Lowry's method --- p.106 / Chapter 3.3.7 --- Medium MDA determination --- p.106 / Chapter 3.3.8 --- Medium GSSG determination --- p.107 / Chapter 3.3.9 --- Antioxidant enzymes measurement --- p.108 / Chapter 3.3.9.1 --- Catalase measurement --- p.108 / Chapter 3.3.9.2 --- SOD measurement --- p.109 / Chapter 3.3.9.3 --- GST measurement --- p.109 / Chapter 3.3.9.4 --- GR measurement --- p.110 / Chapter 3.3.10 --- Statistical analysis --- p.110 / Chapter 3.4 --- Results --- p.111 / Chapter 3.4.1 --- Cytotoxicity of MCCI on rat hepatocytes --- p.111 / Chapter 3.4.2 --- Effect of tBHP and MCCI on hepatocytes viability --- p.111 / Chapter 3.4.3 --- Effects of tBHP and MCCI on hepatocytes GSH and GSSG content --- p.117 / Chapter 3.4.4 --- Effect of MCCI on lipid peroxidation of hepatocytes --- p.121 / Chapter 3.4.5 --- Effect of MCCI on antioxidant enzymes activities --- p.121 / Chapter 3.4.6 --- Comparison with typical antioxidants --- p.125 / Chapter 3.5 --- Discussion --- p.127 / Chapter Chapter 4 --- Cytotoxicity of MCCI on tumor cell lines / Chapter 4.1 --- Introduction --- p.134 / Chapter 4.1.1 --- Relationship between protease inhibitors and cancer --- p.134 / Chapter 4.1.2 --- Cell cycle and apoptosis --- p.137 / Chapter 4.2 --- Rationale of the present study --- p.140 / Chapter 4.3 --- Materials and methods --- p.141 / Chapter 4.3.1 --- Materials --- p.141 / Chapter 4.3.2 --- Cell culture --- p.141 / Chapter 4.3.3 --- MTT assay --- p.142 / Chapter 4.3.4 --- Cell cycle analysis --- p.142 / Chapter 4.3.5 --- DNA fragmentation --- p.143 / Chapter 4.4 --- Results --- p.136 / Chapter 4.4.1 --- Cytotoxicity of MCCI --- p.144 / Chapter 4.4.2 --- Cell cycle and apoptosis analysis --- p.147 / Chapter 4.5 --- Discussion --- p.152 / Conclusion and future perspectives --- p.157 / References --- p.160

abstract / toc / Medical Sciences / Master / Master of Medical Sciences

published_or_final_version / Medical Sciences / Master / Master of Medical Sciences

M. Tech. Entrepreneurship / The purpose of this study is to develop a consumer profile of traditional Chinese medicine consumption in South Africa. The research was based upon a nomethetic survey, a total sample size of 321 respondents was selected according to the convenience sampling technique to participate in the research. Descriptive data analysis was performed indicating that behaviour control and social impact norms primarily influencing the purchase of traditional Chinese medicine.

published_or_final_version / Community Medicine / Master / Master of Public Health