Diets formulated to contain varying ratios of omega 6 to omega 3 fatty acids were fed to exercising yearlings to evaluate bone activity and inflammatory response. Nine Quarter Horse yearlings were arranged within a triplicated 3 X 3 Latin Square experimental design and fed one of three diets. Exercise protocol was designed to stimulate sub-clinical inflammation and normal bone response. Body weight and physical growth measurements were not different between groups (P > 0.05), and feed intake was similar between groups (P > 0.05). Horses consuming soybean oil (SBO) diet had lower fatty acid profiles (% by weight) of C16:0 and C16:1 (P < 0.05) when compared to horses consuming either corn oil (CO) or menhaden/corn oil (MCO) diets. Though numerically different, percentage changes in C16:0 and C16:1 were not different between diets (P < 0.05). Horses consuming MCO had significantly higher measurements of C20:4, C20:5 and C22:6 over the 28 day period when compared to horses consuming SBO or CO. Percent change in mean concentrations of C20:5 were significantly different between the MCO group and the SBO group (P < 0.05) with no observed difference between MCO and CO treatment groups. Overall mean carboxyterminal telopeptide of type I collagen (ICTP) concentrations did not differ between diets (P > 0.05) nor was there a significant change from baseline values when compared to day 28 of the period. Mean Osteocalcin (OC) concentrations did not differ between treatments (P > 0.05). Numerically, OC levels were lower after 14 days, with subsequent increases occurring from day 14 to day 28; however, there was no significant day effect (P > 0.05). Mean measurements of PGE2 and fibrinogen, the two inflammation markers evaluated, did not differ among groups (P > 0.05). However, when fibrinogen data were normalized, horses consuming SBO had a significantly lower change in baseline values of fibrinogen compared to horses fed CO or MCO diets (P< 0.05). In general, horses fed SBO exhibited reduced levels of the inflammatory marker fibrinogen (P< 0.05). No other variable evaluated was influenced by the supplementation of varying ratios of polyunsaturated fatty acids into the equine diet.

Tidal freshwater wetlands provide valuable ecosystem services such as nutrient cycling, coastal flood control, and reproductive habitat for early life stages of economically and ecologically important fishes. The nutrient rich environments that tidal freshwater wetlands occur in support high levels of primary productivity of phytoplankton and vegetation that provide essential reproductive habitat for anadromous clupeid fishes (Alosa spp., Brevoortia tyrannus) in Chesapeake Bay tributaries. Seasonal retention of clupeid eggs and larvae within tidal freshwater habitats may enhance early growth, survival, and year class strength. The primary goal of the present study was to characterize the relative importance of tidal freshwater wetlands to the early life history stages of anadromous, migratory, and resident clupeid fishes in the lower James River. From 2014-2018, conical tow nets were used to collect ichthyoplankton samples from representative locations within the tidal freshwater marsh-creek complex of Curles Neck Creek, Virginia during the period of February through May. Boat electrofishing was used to sample juvenile target clupeids within the marsh-creek complex during March through November 2014-2018. A strong positive correlation between larval and juvenile Blueback Herring and Atlantic Menhaden, supported the hypothesis of seasonal in-system retention. This was the first published study to describe spatial and temporal patterns of occurrence of clupeid fishes with varying life history strategies in a tidal freshwater marsh and highlighted the importance of tidal freshwater marshes as nursery habitat.

Thermally processed menhaden products were evaluated as potential food products. Nine canned menhaden products judged to be satisfactory in pilot studies were canned or pasteurized and evaluated by a six member semi—trained panel. Panelists scored product characteristics of menhaden products and commercially canned tuna in oil, tuna in water and mackerel. Three canned menhaden products were incorporated in "pizza" sauce and in fish salad recipes that were scored for preference by consumer panelists. Objective measurements for drained weight and total fluid were recorded. Descriptive analysis of the nine canned menhaden products indicated that the dressed and filleted menhaden products were similar in the firmness, flakiness, chewiness, moisture, and fish flavor characteristics. Canned minced menhaden products were significantly different from the dressed and filleted fish products in the texture characteristics: firmness, flakiness and chewiness. Plots drawn for comparison of mean characteristic scores of each menhaden product and the three comparison products (tuna in oil, tuna in water, mackerel) indicated that the canned filleted menhaden in oil was judged by a semi—trained panel to be similar to the two commercially canned tuna products. Consumer preference scores for the menhaden products were significantly different from the scores for comparison products used in the pizza sauces and fish salads. The menhaden fish salads and the menhaden pizza sauce were scored lower. Objective measurements indicated that the use of an alum and citric acid brine increased percent weight loss and percent fluid loss. The minced menhaden product brined in alum and citric acid had the highest recorded percent weight loss and percent fluid loss. Pilot study and consumer preference panelists indicated that the alum and citric acid brine imparted a metallic aftertaste to the canned menhaden products. / Master of Science

Recent U.S. legislation applies a precautionary approach to setting catch regulations in federal fisheries management. A transparent approach to complying with federal guidelines involves calculating the catch recommendation that corresponds to a specified probability, P*, of exceeding the "true" overfishing limit (OFL) located within an estimated distribution. The P* methodology aims to manage the risk of overfishing explicitly, but choice of P* alone does not provide sufficient information on all of the risks associated with a control rule—both the probability of overfishing and the severity of overfishing. Rather, the ramifications of P* choices depend on the amount of uncertainty in the stock assessment and on the life history of the species in question. To evaluate these effects on the risks associated with P* rules, my study simulated fishing three example species under three levels of uncertainty. Trends identified among example species were consistent with predictions from life history. Periodic strategists, which have highly variable recruitment, experienced probabilities of overfishing which exceeded P* and which increased in time. Equilibrium strategists showed more predictable risks of overfishing but may have less capacity to recover from depleted biomass levels. Differences in the size of the OFL distribution—representing differences in levels of uncertainty—led to mixed results depending on whether the distribution was biased or whether uncertainty was fully characterized. Lastly, because OFL distributions are themselves estimates and subject to uncertainty in their shape and size, lower P* values closer to the tails of the estimated distribution produced more variable resulting risks. / Master of Science

Preventing oxidative deterioration of fish oil is a significant challenge for the food industry. Natural antioxidants are widely incorporated into foods and oils to prevent oxidation and extend shelf life. The goal of the study is to investigate the activity of novel antioxidants in menhaden oil and to develop optimum formulations containing mixed tocopherols to control oxidation of menhaden oil. Alpha tocopherol, gamma tocopherol, and delta tocopherol in menhaden oil were found at 0.18mg/g, 0.37mg/g, and 0.14mg/g, respectively, using HPLC analysis. Teng Cha extract effectively delayed oxidation of menhaden oil (MO) when stored at 40°C for eight days by measuring primary oxidation products and secondary oxidation products. The combinations of Teng Cha extract and rosemary extract and combinations of ascorbyl palmitate, citric acid, Teng Cha extract and rosemary extract more effectively improved stability of MO containing mixed tocopherols than Teng Cha extract alone at 40°C storage for eight days by measuring primary oxidation products and secondary oxidation products. From this study, Teng Cha extract can be used as a potential natural antioxidant in food industry, especially in combinations with rosemary extract and tocopherols, extending shelf life of menhaden oil. / Master of Science in Life Sciences