This research mainly investigated how the hydrophilic and hydrophobic properties of gas diffusion layer, and the different open ratio of the flowfield may affect the performance of the micro proton exchange membrane fuel cell (£gPEMFC). The flow plate used in this experiment was made through deep UV lithography manufacturing processes and micro-electroforming manufacturing processes. Four different open ratios, 52.8 %, 50.8 %, 75.2 % and 75.75 %, of the flowfield were designed for the flow plate composed of serpentine-parallel and serpentine geometrical micro configurations. Acrylic (PMMA: Polymethylmethacrylate) was used to make the terminal plate placed on both sides of the micro proton exchange membrane fuel cell. By varying values of the hydrophilic and hydrophobic properties of the anode gas diffusion layer, the effects of these two parameters on the polarization curve and power density of the cell were explored. All results obtained in the experiment are presented by P-I curve and V-I curve. The experiment results show that, with 1: 5 flow ratio of anode to cathode, a design with the gas diffusion layer made of the material with hydrophobic factor 20 wt.% and with open ratio of 50.8 % for anode flow channel as well as open ratio of 75.75 % for cathode flow channel may have the best performance.