The H2/air micro PEMFC stacks were designed and fabricated in-house through MEMS (Micro-Electro-Mechanical System) technology with deep UV lithography manufacturing processes (SU-8 photoresist) and micro electroforming manufacturing processes to construct a novel metallic bipolar plate. The effect of different operating parameters on micro PEMFC stacks performance was experimentally investigated for serpentine flow-field configuration. Experiments were conducted through a serious laboratory experiments with different operating conditions of temperature (25oC, 35 oC and 50 oC), anode backpressures (97kPa, 153kPa, 207kPa) as well as anode humidifier temperature (25oC, 35 oC and 50 oC). Experimental results are presented in the form of polarization VI curves and PI curves under above operating conditions. The influence of the aforementioned parameters was presented and discussed.