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Applications of photon correlation spectroscopy and flow cytometry to microbiologyJepras, Robert Ian January 1991 (has links)
No description available.
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Biochemical characterization of 2,4,6-trichlorophenol degradation in bacterium Cupriavidus necator JMP134Belchik, Sara Mae. January 2009 (has links) (PDF)
Thesis (Ph. D.)--Washington State University, May 2009. / Title from PDF title page (viewed on June 4, 2009). "School of Molecular Biosciences." Includes bibliographical references.
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Microbial community ecology in bioelectrochemical systems (BESs) using 16S ribosomal RNA (rRNA) pyrosequencingPark, Tae Jin, 朴台鎮 January 2014 (has links)
abstract / Biological Sciences / Doctoral / Doctor of Philosophy
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Development, optimisation and application of a solid phase system for microbial biosensor deployment in soilsMa, Wei January 2013 (has links)
No description available.
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Cell immobilization techniques for the preservation of probioticsThantsha, Mapitsi Silvester. January 2007 (has links)
Thesis (PhD (Plant Production and Soil Science)--University of Pretoria, 2007. / Includes summary. Includes bibliographical references. Available on the Internet via the World Wide Web.
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Application of microbial biosensors for groundwater and wastewater monitoringSinebe, Brekumoh Sarah January 2015 (has links)
No description available.
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Biohydrogen production by photo fermentation from wastewaterLi, Ruying., 李茹莹. January 2007 (has links)
published_or_final_version / abstract / Civil Engineering / Doctoral / Doctor of Philosophy
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Partial purification and characterisation of Phialophora alba xylanases and its application to pretreated sugarcane bagasse.Mosina, Leticia Ntsoaki. 12 September 2014 (has links)
Xylan is the major component of hemicellulose and its degradation can be achieved through the
hydrolytic action of microbial xylanases. Xylanases have an array of applications one being
bioethanol production. The lack of thermophilic xylanases has prompted the search for new
enzymes with increased thermostability. Previous work on the crude enzyme of Phialophora
alba has demonstrated optimal activity (39 U/μg) at a pH of 4 and two temperature optima of
50°C and 90°C. These desirable properties highlighted the need for further research on the
purified enzyme. In the present study P. alba was identified as a thermophilc Ascomycete that
forms conidia and chlamydospores during the asexual and sexual stages of its life cycle,
respectively. The various isozymes present in the crude enzyme extract were subsequently
detected by zymogram analysis. Up to six xylanase isozymes ranging from 90-210 kDa in size
were detected. The crude enzyme was subsequently purified by precipitation and ion exchange
chromatography (IEX). Protein precipitation methods, desalting methods, IEX resins, elution
buffers and NaCl gradients were optimized. The 31-70% ammonium sulphate precipitate had the
highest levels of xylanase activity. Separation of proteins with the anion exchanger, HiTrap Q
sepharose fast flow column and a linear gradient of 0-2.5 M NaCl in phosphate buffer (50 mM,
pH 7) yielded a partially pure xylanase isozyme with molecular weight of 210 kDa. A final yield
of 1.4% and purification fold 10.6 was obtained after ion exchange chromatography. The specific
activity of the xylanase was 21 IU/μg. At optimum pH (pH 4) and temperature (50°C) a
combined xylanase activity of 32 IU.ml⁻¹ was detected. The partially pure xylanase was stable
from pH 4-6 with 86% of xylanase activity retained for 90 minutes. Thermostability was
observed from 40-70°C with 95% of activity retained for 90 minutes at optimum temperature.
The ability of the partially pure xylanase and crude enzyme to hydrolyze untreated and pretreated (alkali and temperature/pressure) sugarcane bagasse was tested at a constant enzyme loading rate
of 15 IU/g. Overall, maximum hydrolysis was achieved with the alkali pretreatment and
saccharification with the crude enzyme: approximately, 2.4 g/ml of reducing sugars were
liberated over a 48 hours. The partially pure xylanase liberated a maximum amount of 2.3 g/ml
reducing sugars after 48 hours. The results obtained highlight the desirable characteristics of the
partially pure enzyme and its applicability to bioethanol production. / Thesis (M.Sc.)-University of KwaZulu-Natal, Durban, 2013.
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Biohydrogen production by photo fermentation from wastewaterLi, Ruying. January 2007 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2008. / Also available in print.
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Biological leaching of shales : black shale and oil shale /Tasa, Andrus. January 1998 (has links)
Thesis (doctoral)--University of Tartu, 1998. / Includes bibliographical references.
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