<p dir="ltr">Aquaculture is a controlled aquatic farming sector and one of the most important human food sources. Fish farming is one of the predominant, fast-growing sectors that supply seafood products worldwide. Along with its benefits, aquaculture practices can discharge large quantities of nutrients into the environment through non-treated or poorly treated wastewater. This study aims to understand the nutrient composition of fish wastewater and the use of indigenous bacteria, cyanobacteria, and microalgae as an alternative biological treatment method. Wastewater samples from a local fish farming facility were collected and treated using six different species of cyanobacteria and microalgae include <i>Chroococcus</i><i> </i><i>minutus</i>, <i>Porphyridium</i><i> </i><i>cruentum</i>, <i>Chlorella vulgaris</i>, <i>Microcystis aeruginosa</i>, <i>Chlamydomonas </i><i>reinhardtii</i>, and <i>Fischerella</i><i> </i><i>muscicola</i>. All the samples were incubated for 21 days, and the following parameters were measured weekly: Chemical oxygen demand (COD), phosphate, total dissolved nitrogen, and dissolved inorganic nitrogen. In addition, dissolved organic nitrogen (DON), bioavailable DON (ABDON), and biodegradable DON (BDON) were calculated from the mass-balance equations. Colorimetric and digestive methods were used for the parameter measurements. The results showed that <i>C. </i><i>reinhardtii</i> reduced the soluble COD concentration by 74.6%, DON by 94.3%, and phosphorous by more than 99%. Moreover, <i>M. aeruginosa</i>, and <i>C. </i><i>minutus</i> significantly reduced inorganic nitrogen species (>99%). This alternative fish wastewater treatment method was explored to gain insight into fish wastewater nutrient composition and to create a sustainable alternative to conventional fish wastewater treatment methods.<b> </b></p>