• Refine Query
  • Source
  • Publication year
  • to
  • Language
  • 2
  • Tagged with
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Population genetics of Colletotrichum truncatum associated with soybean anthracnose / Genética populacional de Colletotrichum truncatum associado à antracnose da soja

Rogério, Flávia 05 July 2019 (has links)
The soybean crop is one of the main agricultural crops, with high global economic relevance. The large area under soybean cultivation in Brazil, including the incorporation of new areas in the northern and midwestern regions, mostly under monoculture and non-tillage system, has been affected the prevalence and the intensity of diseases. Among these, one of most prominent is anthracnose, mainly associated with the fungal species Colletotrichum truncatum. Knowledge of the genetic structure of plant pathogen populations can be used to infer their life histories and the evolutionary processes that shape populations in the agroecosystems, which can help to implement effective disease management strategies. However, the genetic structure of C. truncatum populations associated with soybean remains unknown. We collected C. truncatum isolates from 10 sites representing two of main areas of soybean producing in Brazil and used microsatellite markers and whole-genome sequencing to investigate the population biology and evolutionary history of this important pathogen. The multilocus microsatellite typing of 237 isolates revealed high gene and haplotypic diversity within populations, as well low genetic differentiation and sharing of multilocus haplotypes among populations and regions. In addition, three distinct genetic clusters were detected, coexisting in syntopy in the soybean fields, without evidence of admixture between them. Such finding suggesting that Brazilian C. truncatum populations resulted from at least three founder events, which led to three genetic lineages that spread throughout the country. However, the genetic makeup of these lineages remains unknow, and their extreme geographic proximity raises the question of the maintenance of their genetic integrity in the face of admixture. In order to gain insights into the evolutionary history of C. truncatum lineages and to investigate in more details the possibility of a lack of genetic exchanges between them, we employed a population genomic approach. For that, we produced a draft genome sequence of a typical strain of the species associated with soybean anthracnose, which was used as the reference genome. Eighteen representative C. truncatum isolates from the three lineages were submitted to whole genome sequencing, aligned against the reference genome, and variants were identified. Our population genomic analyzes revealed that the genetic structure of C. truncatum pathogen causing soybean anthracnose is formed by three deeply divergent lineages with levels of genetic diversity consistent with repeated introduction events for each lineage. We also found evidence for sexual recombination within and between lineages, with multiples isolates displaying signatures of admixture. Our findings support a scenario in which the three lineages initially diverged in allopatry before experiencing hybridization following secondary contact. Monitoring of the pathogen\'s diversity over time is needed to reveal whether these lineages maintain or fuse, which can impact the disease control methods currently employed. / A soja é uma das principais culturas agrícolas, com alta relevância econômica global. A grande área sob cultivo de soja no Brasil, incluindo a incorporação de novas áreas nas regiões norte e centro-oeste, principalmente sob monocultura e plantio direto, tem afetado a prevalência e a intensidade das doenças. Entre elas, uma das mais proeminentes é a antracnose, principalmente associada à espécie fúngica Colletotrichum truncatum. O conhecimento da estrutura genética das populações de patógenos de plantas pode ser usado para inferir suas histórias de vida e os processos evolutivos que moldam as populações nos agroecossistemas, o que pode ajudar a implementar estratégias eficazes de manejo da doença. No entanto, a estrutura genética das populações de C. truncatum associadas à soja permanece desconhecida. Coletamos isolados de C. truncatum em 10 áreas, representando duas principais regiões produtoras de soja no Brasil. Utilizamos marcadores microssatélites e sequenciamento do genoma completo para investigar a biologia populacional e a história evolutiva desse importante patógeno. A tipagem de microssatélites multilocus de 237 isolados revelou alta diversidade genética e haplotípica nas populações, bem como baixa diferenciação genética e compartilhamento de haplótipos entre populações e regiões. Além disso, foram detectados três grupos genéticos distintos, coexistindo nas mesmas áreas, sem evidência de mistura entre eles. Isto sugere que as populações C. truncatum no Brasil resultaram de pelo menos três eventos fundadores, o que levou á formação das três linhagens genéticas que se espalharam pelo país. No entanto, a composição genética dessas linhagens permanece desconhecida, e sua extrema proximidade geográfica levanta a questão sobre a manutenção de sua integridade genética em face a mistura entre elas. A fim de analisar a história evolutiva das linhagens de C. truncatum e investigar a possibilidade de ausência de trocas genéticas entre elas, empregamos uma abordagem genômica populacional. Para isso, produzimos uma versão preliminar do genoma completo de um isolado típico da espécie, o qual foi utilizado como genoma de referência. Dezoito isolados representativos das três linhagens foram submetidos ao sequenciamento completo, alinhados ao genoma de referência, e variantes foram identificados. Nossas análises genômicas populacionais revelaram que a estrutura genética do patógeno é formada por três linhagens profundamente divergentes, com níveis de diversidade consistentes com repetidos eventos de introdução para cada linhagem. Também encontramos evidências de recombinação sexual dentro e entre linhagens, com múltiplos isolados apresentando assinaturas de mistura. Nossas descobertas sugerem um cenário no qual as três linhagens divergiram inicialmente em alopatria antes de experimentar hibridação, após contato secundário. O monitoramento da diversidade do patógeno ao longo do tempo é necessário para revelar se essas linhagens se mantêm geneticamente separadas ou se fundem, o que pode afetar os métodos de controle da doença atualmente empregados.
2

Phylogeography, population structure and distribution of genetic variation across the Leishmania donovani species complex with emphasis on the Indian subcontinent

Stark, Olivia 10 March 2017 (has links)
Erreger des Leishmania donovani Komplexes (LDC) verursachen viszerale Leishmaniose (VL), eine der häufigsten durch Sandmücken übertragenen Infektionskrankheiten beim Menschen. Die vorliegende von der EU geförderte Dissertation untersucht die weltweite Populationsstruktur des LDC mit besonderem Schwerpunkt auf dem Indischen Subkontinent (ISC), wo das gehäufte Auftreten von Therapieversagen ein Problem für die geplante Eliminierung von VL darstellt. Zwei hoch diskriminierende molekulare Typisierungsverfahren wurden angewendet. 845 LDC-Isolate wurden mittels Multi-Lokus-Mikrosatelliten-Typisierung (MLMT) charakterisiert. Die Parasiten wurden in Gebieten mit endemischer VL aus unterschiedlichen Wirten isoliert und repräsentieren verschiedene klinische Formen der Leishmaniose. Eine 125 Parasiten umfassende Teilprobe wurde vollständig sequenziert und in einem next-generation Multi-Lokus-Sequenz-Ansatz (ng-MLSA) typisiert, welcher auf der Analyse von genomweiten Single-Nukleotid-Polymorphismen (SNP) beruht. Sowohl die MLMT- als auch die SNP-Daten wurden mit den gleichen populationsgenetischen Methoden ausgewertet. Der ng-MLSA Ansatz bestätigte weitgehend die Populationsstrukturen des mit dem MLMT analysierten größeren Datensatzes, die genetische Struktur korrelierte mit der geographischen Herkunft der Isolate. Die Unempfänglichkeit der Parasiten gegenüber Antimon- oder Miltefosin sowie die in vitro gemessene Resistenz der Isolate vom ISC konnten nicht auf einen spezifischen Genotyp zurückgeführt oder mit einem spezifischen genetischen Merkmal verknüpft werden. Die Gesamtgenomsequenzierung konnte bisher keine Mutationen im Parasitengenom nachweisen, die in Zusammenhang mit der Antimon- und Miltefosin-Unempfindlichkeit bzw. dem Therapieversagen gebracht werden könnten. Analysen basierend auf ausgewählten Sequenzen deuten auf eine variable chromosomale Ploidie und eine erhöhte Kopienzahl einiger Gene hin, die zur Entstehung von Arzneimittelresistenzen beitragen könnten. / Parasites of the Leishmania donovani species complex (LDC) cause most cases of visceral leishmaniasis (VL), one of the most fatal vector-borne parasitic human diseases. As part of an EU funded project, this dissertation has investigated the worldwide genetic population structure of parasites of the LDC, with special focus on the Indian subcontinent (ISC) where unresponsiveness to anti-leishmanial drugs has recently become an urgent problem for the containment of VL. Two types of highly discriminatory approaches have been used. Multi-locus microsatellite typing (MLMT) has been applied to 845 LDC isolates from numerous Old and New World foci of VL, from different clinical forms of the disease and from various hosts. A subset of 125 fully sequenced isolates, reflecting the worldwide distribution of the LDC, was analysed using a next-generation multi-locus sequence approach (ng-MLSA) including single nucleotide polymorphisms (SNP). Both microsatellite and SNP data sets were analysed using, in general, the same population genetic tools. The ng-MLSA approach has, in general, corroborated the population structures obtained with MLMT for the larger data set. With the exception of non MON-1 parasites, the genetic structure revealed was largely associated with the geographic origin of the isolates, but not with the clinical presentation, host specificity and the immune status of the host or year of parasite isolation. Unresponsiveness to antimony or miltefosine treatment as well as the respective resistances measured in vitro could not be linked to a specific genotype or genetic trait. Wg sequencing also failed, so far, to identify mutations, which could be related to the unresponsiveness of LDC isolates from the ISC to antimony and miltefosine therapy. Analyses of selected targets have revealed extensive variation in chromosomal ploidy in all wg sequenced isolates under study and copy number variations for some genes possibly involved in drug resistance.

Page generated in 0.0646 seconds