Short rotation woody crops (SRWC) eastern cottonwood (Populus deltoides) and hybrid poplar plantations were established in 2021 in Pontotoc and Oktibbeha counties of Mississippi to study the biomass potential of SRWC for biofuel production. We used a novel backpack LiDAR system to measure forest metrics and harvested sample trees to build aboveground biomass (AGB) and leaf area index (LAI) equations. The results showed that LiDAR-derived variables accurately estimated aboveground biomass (R2 =0.81 and 29.22 % RMSE). However, the LAI estimation results showed that the LiDAR metrics moderately explained field measurements of LAI (R2 =0.31 and 18.05% RMSE) for individual-trees and poorly explained plot-level LAI measured with the LAI-2200C (R2 =0.11 and 66% RMSE). The backpack LiDAR system can be valuable for forest managers and researchers, enabling non-destructive AGB and LAI estimation. However, further research is required to overcome its limitations and achieve precise measurements of AGB and LAI.