The mixing layer (ML) heights inferred from radiosondes, wind profilers, airborne lidar, airborne microwave temperature profiler (MTP), and in-situ aircraft data were compared during the Texas 2000 Air Quality Study in the Houston area. The comparisons and resulting good agreement between the separate instruments allowed for the spatial and temporal evolution of the ML height distribution to be determined across the Houston area on September 1, 2000. A benchmark method was created for determining ML heights from radiosonde data. The ML heights determined using this method were compared to ML heights determined using wind profiler data. The airborne lidar and MTP heights were also compared to the wind profiler heights. This was the first time the MTP was used for estimating ML heights. Because of this, the MTP heights were also compared to the ML heights determined by in-situ aircraft data. There was good agreement between the ML estimates when the instruments were co-located. The comparisons between the benchmark method and the wind profilers were independent of the quality of the profiler heights. The statistics for lidar and the wind profilers were better for the inland profiler comparisons. Even so, the results for coastal profilers were similar to the other comparisons. The results between the MTP and the wind profilers were comparable with the results found between the other instruments, and better, in that the statistics were similar for the both the inland and coastal profilers. The results between the MTP and in-situ aircraft data provided additional support for the use of MTP for determining ML heights. The combination of the inland and coastal wind profilers with the airborne instruments provided adequate information for the spatial and temporal evolution of the ML height to be determined across the Houston area on September 1, 2000. By analyzing the ML height distribution, major features were evident. These features included the shallow ML heights associated with the marine air from Galveston Bay and the Gulf of Mexico, and the sharp gradient of increasing ML heights north of Houston associated with the variation in the inversion depth found on this day.