<p>Stress responsive genes play a key role in shaping the manner in which plants process and respond to environmental stress. Their gene products are linked to DNA transcription and its consequent translation into a response product. However, whilst these genes play a significant role in manufacturing responses to stressful stimuli, transcription factors coordinate access to these genes, specifically by accessing a gene&rsquo / s promoter region which houses transcription factor binding sites. Here transcriptional elements play a key role in mediating responses to environmental stress where each transcription factor binding site may constitute a potential response to a stress signal. Arabidopsis thaliana, a model organism, can be used to identify the mechanism of how transcription factors shape a plant&rsquo / s survival in a stressful environment. Whilst there are numerous plant stress research groups, globally there is a shortage of publicly available stress responsive gene databases. In addition a number of previous databases such as the Generation Challenge Programme&rsquo / s comparative plant stressresponsive gene catalogue, Stresslink and DRASTIC have become defunct whilst others have stagnated. There is currently a single Arabidopsis thaliana stress response database called STIFDB which was launched in 2008 and only covers abiotic stresses as handled by major abiotic stress responsive transcription factor families. Its data was sourced from microarray expression databases, contains numerous omissions as well as numerous erroneous entries and has not been updated since its inception.The Dragon Arabidopsis Stress Transcription Factor database (DASTF) was developed in response to the current lack of stress response gene resources. A total of 2333 entries were downloaded from SWISSPROT, manually curated and imported into DASTF. The entries represent 424 transcription factor families. Each entry has a corresponding SWISSPROT, ENTREZ GENBANK and TAIR accession number. The 5&rsquo / untranslated regions (UTR) of 417 families were scanned against TRANSFAC&rsquo / s binding site catalogue to identify binding sites. The relational database consists of two tables, namely a transcription factor table and a transcription factor family table called DASTF_TF and TF_Family respectively. Using a two-tier client-server architecture, a webserver was built with PHP, APACHE and MYSQL and the data was loaded into these tables with a PYTHON script. The DASTF database contains 60 entries which correspond to biotic stress and 167 correspond to abiotic stress while 2106 respond to biotic and/or abiotic stress. Users can search the database using text, family, chromosome and stress type search options. Online tools have been integrated into the DASTF&nbsp / database, such as HMMER, CLUSTALW, BLAST and HYDROCALCULATOR. User&rsquo / s can upload sequences to identify which transcription factor family their sequences belong to by using HMMER. The website can be accessed at http://apps.sanbi.ac.za/dastf/ and two updates per year are envisaged.</p>

<p>Stress responsive genes play a key role in shaping the manner in which plants process and respond to environmental stress. Their gene products are linked to DNA transcription and its consequent translation into a response product. However, whilst these genes play a significant role in manufacturing responses to stressful stimuli, transcription factors coordinate access to these genes, specifically by accessing a gene&rsquo / s promoter region which houses transcription factor binding sites. Here transcriptional elements play a key role in mediating responses to environmental stress where each transcription factor binding site may constitute a potential response to a stress signal. Arabidopsis thaliana, a model organism, can be used to identify the mechanism of how transcription factors shape a plant&rsquo / s survival in a stressful environment. Whilst there are numerous plant stress research groups, globally there is a shortage of publicly available stress responsive gene databases. In addition a number of previous databases such as the Generation Challenge Programme&rsquo / s comparative plant stressresponsive gene catalogue, Stresslink and DRASTIC have become defunct whilst others have stagnated. There is currently a single Arabidopsis thaliana stress response database called STIFDB which was launched in 2008 and only covers abiotic stresses as handled by major abiotic stress responsive transcription factor families. Its data was sourced from microarray expression databases, contains numerous omissions as well as numerous erroneous entries and has not been updated since its inception.The Dragon Arabidopsis Stress Transcription Factor database (DASTF) was developed in response to the current lack of stress response gene resources. A total of 2333 entries were downloaded from SWISSPROT, manually curated and imported into DASTF. The entries represent 424 transcription factor families. Each entry has a corresponding SWISSPROT, ENTREZ GENBANK and TAIR accession number. The 5&rsquo / untranslated regions (UTR) of 417 families were scanned against TRANSFAC&rsquo / s binding site catalogue to identify binding sites. The relational database consists of two tables, namely a transcription factor table and a transcription factor family table called DASTF_TF and TF_Family respectively. Using a two-tier client-server architecture, a webserver was built with PHP, APACHE and MYSQL and the data was loaded into these tables with a PYTHON script. The DASTF database contains 60 entries which correspond to biotic stress and 167 correspond to abiotic stress while 2106 respond to biotic and/or abiotic stress. Users can search the database using text, family, chromosome and stress type search options. Online tools have been integrated into the DASTF&nbsp / database, such as HMMER, CLUSTALW, BLAST and HYDROCALCULATOR. User&rsquo / s can upload sequences to identify which transcription factor family their sequences belong to by using HMMER. The website can be accessed at http://apps.sanbi.ac.za/dastf/ and two updates per year are envisaged.</p>

Made available in DSpace on 2016-06-02T19:52:10Z (GMT). No. of bitstreams: 1 DissFPCS.pdf: 3175020 bytes, checksum: cd2ef75652e3937ea059b12ac168afcb (MD5) Previous issue date: 2005-06-06 / The purpose of this study is to investigate the implementation of Enterprise Resource Planning (ERP) in small and medium enterprises (SME) in whom it says respect to the Business Processes Modeling (BPM) use. Until little time behind, the SME were not using conventionals of system ERP. Observed the BPM use, for the suppliers, and verified it influence in the integration of the internal processes of the business of the customer. Due to the fact that the utilization of (ERP) in small and medium enterprises is a recent issue, most of the reviewed literature was found in articles and books addressed to big companies. Substantive matters on business processes modeling (BPM) were found in international articles and books. Hardly ever, BPM, ERP or SME issues were discussed in the same article or book. Therefore, for this inquiry, one searched the information in the suppliers and customers of ERP, by means of differentiated questionnaires, that had been applied in the national supplying greaters of systems and in some customers of the system of these suppliers. This research contributed with the systematization of theoretical information regarding the concepts of BPM and in the vision of the ERP sustem in SME. With the carried through research of field, it made possible to get real information of the main characteristics of implementation with the use of the BPM for the suppliers and of its interference in the internal processes of business of some using customers, showing indications of that the BPM contributes for a bigger integration of the internal processes of business and that this option is made by the customer and that all the suppliers of this research had conditions to take care of it. / A proposta deste trabalho é de investigar a implementação de sistemas ERP (Enterprise Resource Planning) em pequenas e médias empresas (PMEs), no que diz respeito ao uso de modelagem de processos de negócios (MPN). Até pouco tempo atrás, as PMEs não eram usuárias convencionais de sistema ERP. Observou-se o uso de modelagem de processos de negócios (MPN), pelos fornecedores, e verificou-se a influencia na integração dos processos internos do negócio do cliente. Por se tratar de um assunto recente, no que diz respeito á utilização de ERP em PMEs, a literatura encontrada foi, em grande parte, de artigos e livros direcionados para as grandes empresas. Quanto a MPN foram encontrados artigos e livros, principalmente, de publicações internacionais. Raramente, os assuntos de MPN e ERP em PMEs estavam num mesmo artigo ou livro. Portanto, para esta investigação, buscou-se as informações nos fornecedores e clientes de ERP, por meio de questionários diferenciados, que foram aplicados nos maiores fornecedores nacionais de ERP e em alguns clientes do sistema destes fornecedores. Esta pesquisa contribuiu com a sistematização de informações teóricas a respeito dos conceitos de MPN e na visão do sistema ERP nas PMEs. Com a pesquisa de campo realizada, foi possível obter informações reais das principais características de implementação com o uso da MPN pelos fornecedores e de sua interferência nos processos internos de negócio de alguns clientes usuários, mostrando indícios de que a MPN contribui para uma maior integração dos processos internos de negócio e que esta opção é feita pelo cliente e que todos os fornecedores desta pesquisa tinham condições de atendê-la.