Submitted by PPG Zoologia (zoologia-pg@pucrs.br) on 2018-08-01T18:22:00Z No. of bitstreams: 1 Almeida MAB___TESE___VERS?O FINAL.pdf: 2953118 bytes, checksum: 99cbefa9c38c7969abce4bafc4b20d54 (MD5) / Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2018-08-02T17:55:11Z (GMT) No. of bitstreams: 1 Almeida MAB___TESE___VERS?O FINAL.pdf: 2953118 bytes, checksum: 99cbefa9c38c7969abce4bafc4b20d54 (MD5) / Made available in DSpace on 2018-08-02T18:39:41Z (GMT). No. of bitstreams: 1 Almeida MAB___TESE___VERS?O FINAL.pdf: 2953118 bytes, checksum: 99cbefa9c38c7969abce4bafc4b20d54 (MD5) Previous issue date: 2018-03-22 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Nonhuman primates (NHP) are susceptible to many arboviruses, including the yellow fever (YF) virus. Although native to Africa, this virus found susceptible NHP and competent mosquito vectors for maintaining its transmission in American forests. A high sensitivity of NHP to YF led health agencies to monitor these animals as a way of monitoring the disease in Brazil. The State of Rio Grande do Sul (RS) began this surveillance in 2002, which has detected the arboviruses Oropouche and Saint Louis (SLEV) and a YF epizootic that killed more than 2,000 NHP (Alouatta caraya and A. guariba clamitans) between 2008 and 2009. The objectives of this PhD thesis research were to generate models of niche suitability for YF based on that epizootic and prospect arboviruses in NHP in northwestern RS. The maximum entropy algorithm - Maxent was used to generate distribution models of Alouatta spp. and the mosquito vector Haemagogus leucocelaenus. Together with climatic, topographic and vegetative variables, these models served as predictor layers to model the occurrence of the disease based on the points of death of NHP of YF. The most influential variables in the YF models were the variation in air humidity, distribution of Alouatta spp. and maximum wind speed followed by mean annual rainfall and maximum temperature. Therefore, support for the influence of the rainfall regime and the ambient temperature on the cycle of jungle YF was found. Wind speed and direction can play an important role in the dispersal of infected mosquitoes and, consequently, the virus. The models based on the occurrence of dead NHP in the first months of the epizootic identified suitable areas to where the disease spread a few months later. In addition, 19 arboviruses were prospected in 40 blood (viral isolation and PCR) and serum (hemagglutination inhibition and neutralization tests [NT]) samples collected from 26 black howler monkeys (A. caraya) belonging to three populations in four field campaigns in the municipality of Santo Ant?nio das Miss?es, RS, between 2014 and 2016. There was no detection of circulating virus, but antibodies to Flavivirus SLEV and Ilh?us and Phlebovirus Icoaraci was found by NT. Evidence of the contact with Ilh?us and Icoaraci are the southernmost records in Brazilian NHP. An increase in antibodies to SLEV detected between two consecutive captures of the same individual is compatible with a recent contact with the virus. An adult male captured in one of the areas presented concomitant infection by the Oropouche, SLEV and YF viruses by NT. Further studies are necessary to understand the role played by NHP and other vertebrates in the circulation of arboviruses in the region, to assess potential risks to NHP and public health, and to identify the driving forces responsible for the dispersal of the YF virus during epizootics in wildlife populations. / Os primatas n?o-humanos (PNH) s?o suscet?veis a diversos arbov?rus, incluindo o v?rus da febre amarela (FA). Embora origin?rio da ?frica, esse v?rus encontrou PNH suscet?veis e mosquitos vetores competentes para sua transmiss?o em matas nas Am?ricas. Uma alta sensibilidade dos PNH ? FA levou ?rg?os de sa?de a monitorar esses animais como forma de vigiar a doen?a no Brasil. O Estado do Rio Grande do Sul (RS) iniciou essa vigil?ncia em 2002, a qual detectou os arbov?rus Oropouche e Saint Louis (SLEV) e uma epizootia de FA que matou mais de 2000 PNH (Alouatta caraya e A. guariba clamitans) entre 2008 e 2009. A presente tese de doutorado teve como objetivos gerar modelos de adequabilidade ambiental para FA com base nessa epizootia e prospectar arbov?rus em PNH no noroeste do RS. Foi utilizado o algoritmo de m?xima entropia ? Maxent para gerar modelos de distribui??o de Alouatta spp. e do mosquito vetor Haemagogus leucocelaenus. Esses modelos serviram como camadas preditoras para, junto a vari?veis clim?ticas, topogr?ficas e vegetacionais, modelar a ocorr?ncia da doen?a baseada nos pontos de morte de PNH por FA. As vari?veis mais influentes nos modelos da FA foram a varia??o na umidade do ar, a distribui??o de Alouatta spp. e a velocidade m?xima dos ventos, seguidas pela precipita??o m?dia anual e a temperatura m?xima. Portanto, foi confirmado suporte para a influ?ncia do regime de chuvas e da temperatura ambiente no ciclo da FA silvestre. A velocidade e a dire??o do vento devem desempenhar um importante papel na dispers?o de mosquitos infectados e, consequentemente, do v?rus. Os modelos baseados na distribui??o espacial de PNH mortos nos primeiros meses da epizootia identificaram ?reas adequadas para onde a doen?a avan?ou poucos meses mais tarde. Tamb?m foram prospectados 19 arbov?rus em 40 amostras de sangue (isolamento viral e PCR) e soro (inibi??o da hemaglutina??o e testes de neutraliza??o [NT]) coletadas em quatro campanhas de campo entre 2014 e 2016 de 26 bugios-pretos (A. caraya) de tr?s popula??es no munic?pio de Santo Ant?nio das Miss?es, RS. N?o houve detec??o de v?rus circulante, mas sim de anticorpos para os Flavivirus SLEV e Ilh?us e o Phlebovirus Icoaraci por NT. As evid?ncias de contato com Ilh?us e Icoaraci s?o as primeiras em PNH no extremo sul do Brasil. Um aumento de anticorpos para SLEV detectado entre duas capturas consecutivas do mesmo indiv?duo ? compat?vel com um contato recente com o v?rus. Um macho adulto capturado em uma das ?reas apresentou infec??o concomitante pelos v?rus Oropouche, SLEV e FA por NT. Mais estudos s?o necess?rios para compreender o papel de PNH e outros vertebrados na circula??o de arbov?rus na regi?o, avaliar poss?veis riscos para PNH e a sa?de humana e identificar as for?as motrizes respons?veis pela dispers?o do v?rus da FA durante epizootias em popula??es selvagens.