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Molecular cloning and characterization of mouse testin complementary DNA張朝雄, Cheung, Chiu-hung. January 1999 (has links)
published_or_final_version / Zoology / Master / Master of Philosophy
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Molecular cloning, expression and characterization of antigenic polypeptides from the human blood fluke schistosoma japonicumMa, Liang, 馬亮 January 2001 (has links)
published_or_final_version / Microbiology / Master / Master of Philosophy
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Molecular cloning and functional characterization of a goldfish glucagon-like receptor莫佩兒, Mok, Pui-yee. January 1997 (has links)
published_or_final_version / Zoology / Master / Master of Philosophy
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Molecular cloning of 1-aminocyclopropane-1-carboxylic acid n-malonyltransferase of mung bean文汝斌, Man, Yu-bun. January 2001 (has links)
published_or_final_version / Zoology / Master / Master of Philosophy
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Characterization and molecular cloning of superoxide dismutases of Trichinella pseudospiralis (nematoda)胡偉光, Wu, Wai-kwong. January 2002 (has links)
published_or_final_version / Zoology / Master / Master of Philosophy
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Foldback DNA: nucleotide sequence and characterization of MboII repeated sequences in human long foldbackDNA by molecular cloning and hybridizationLee, Hong-seng, Daniel, 李康善 January 1987 (has links)
published_or_final_version / Pathology / Doctoral / Doctor of Philosophy
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Cloning of an embryo-specific gene from Daucas Carota cDNA libraryZvejnieks, Peter Andrew 12 1900 (has links)
No description available.
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Molecular cloning and characterization of a novel developmental gene from loblolly pine - a new plant regulatory systemTulsyan, Anurag S. 08 1900 (has links)
No description available.
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Molecular cloning and characterisation of a putative peroxidase cDNA from flax (Linum usitatissimum L.)Beaulieu, Normand January 1989 (has links)
The corresponding isoperoxidases from the flax genotrophs L and S have different molecular weights. Since further structural analysis will rely on knowledge of the primary structures of these molecules, the isolation of a molecular clone of this enzyme was attempted. Probing a cDNA library with a synthetic oligonucleotide was not successful, but a partial cDNA sequence was isolated by probing an S genotroph stem $ lambda$gt11 library with polyclonal antibodies raised against anionic plant peroxidases. Its longest reading frame sequence is divergent from those of other peroxidases, but contains some related segments. Genomic Southern analysis of the clone is presented, along with a study of the expression of its corresponding mRNA in plant tissues. A study on the effects of IAA, ABA and kinetin levels on the expression of peroxidase and of the putative peroxidase cloned mRNA is also presented.
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Identification and characterisation of novel cellulolytic genes using metagenomicsHu, Xiao Ping January 2010 (has links)
<p>Metagenomics has been successfully used to discover novel enzymes from uncultured microorganisms in the environment. In this study, metagenomic DNA from a Malawian hot spring soil sample was used to construct a fosmid library. This metagenomic library comprised of more than 10000 clones with an average insert size of 30 kb, representing more than 3.0 x 108 bp of metagenomic DNA (equivalent to approximately 100 bacterial genomes). The library was screened for cellulase activity using a Congo red plate assay to detect zones of carboxymethylcellulose hydrolysis. This yielded 15 positive fosmid clones, of which five were further characterised for activity and thermostability using the 3, 5-dinitrosalicylic assay. Two of the five fosmids (XP008C2 and XP026G5) were selected for DNA pyrosequencing. The full sequence of the XP008C2 (29800bp) fosmid insert is presented in this study and genes thereon were chosen for further study.</p>
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