Thesis (M.S.)--Duquesne University, 2003. / Title from document title page. Abstract included in electronic submission form. Includes bibliographical references (p. 90-95) and abstract.

Thesis (Ph. D.)--University of Texas at Austin, 2002. / Vita. Includes bibliographical references. Available also from UMI Company.

Thesis (M. Phil.)--University of Hong Kong, 1998. / Includes bibliographical references.

Thesis (M. Phil.)--University of Hong Kong, 2009. / Includes bibliographical references (leaves 92-109) Also available online.

No description available.

Note:

published_or_final_version / Biochemistry / Doctoral / Doctor of Philosophy

published_or_final_version / Zoology / Doctoral / Doctor of Philosophy

published_or_final_version / Zoology / Master / Master of Philosophy

In order to characterize the protein product of the major capsid protein gene of Bovine Papillomavirus Type 2, 93% of the L1 open reading frame was cloned into two different expression vectors. This coding sequence produced a hybrid product when cloned into the expression vector pKK233-2, which interacted weakly with anti-BPV antisera and proved unable to elicit neutralizing antibodies. When the sequence was cloned into the expression vector pRA10, a more native form of the major capsid protein was produced, which interacted well with anti-BPV sera. Antisera raised against this cloned product was able to neutralize BPV in a tissue culture transformation assay. The 3' end of the L1 open reading frame was cloned into pBA10 in order to characterize the immunogenic potential of the carboxy terminus of the major capsid gene. The carboxy terminus proved to have no greater ability to interact with anti-BPV antisera, showing that the immunogenic epitopes of the protein are probably evenly distributed along the linear sequence.