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Molecular cloning, expression and characterization of antigenic polypeptides from the human blood fluke schistosoma japonicum /Ma, Liang. January 2001 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 180-211).
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Foldback DNA : nucleotide sequence and characterization of MboII repeated sequences in human long foldback DNA by molecular cloning and hybridization /Lee, Hong-seng, Daniel. January 1987 (has links)
Thesis (Ph. D.)--University of Hong Kong, 1988.
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Characterization and molecular cloning of superoxide dismutases of Trichinella pseudospiralis (nematoda) /Wu, Wai-kwong. January 2002 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2002. / Includes bibliographical references (leaves 121-131).
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Cloning expression and characterization of human oviductal C3 fragmentsKwok, Ka-leung., 郭家亮. January 2005 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
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The role of endoderm in vascular patterningVokes, Steven Alexander 28 August 2008 (has links)
Not available / text
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Cloning and functional characterization of the WdSTUA and WdPACC genes of Wangiella dermatitidisWang, Qin, 1970 Nov. 15- 28 August 2008 (has links)
To study the function of WdStuAp and WdPacCp in Wangiella dermatitidis, a black, polymorphic fungal pathogen of humans with yeast phase predominance, WdSTUA and WdPACC were cloned, sequenced, disrupted and expressed. WdStuAp was most similar to the APSES proteins of Aspergillus species and its APSES DNA-binding domain was located in its N-terminal half. Deletion of WdSTUA in W. dermatitidis induced convoluted instead of normal smooth colony surface growth on the rich, yeast maintenance agar medium, YPDA, at 37°C. Additionally, deletion of WdSTUA repressed aerial hyphal growth, conidiation and invasive hyphal growth on the nitrogen poor, hyphae-inducing agar medium, PDA, at 25°C. Ectopic expression of WdSTU Arepressed the convoluted colony surface growth on YPDA at 37°C, and also strongly repressed hyphal growth on PDA at 25°C and 37°C. Expression of WdSTUA in S. cerevisiae induced pseudohyphal growth on the nitrogen poor medium. WdPacCp was also most similar to the PacCp proteins of Aspergillus species. Three zinc finger DNA-binding motifs were at the N-terminus, and the C-terminus had the signaling protease cleavage site. WdPACC was more expressed at neutral-alkaline pH than at acidic pH. Truncation of the coding sequence for about 40 residues upstream of the conserved processing protease cleavage site of WdPacCp affected growth on YPDA, increased sensitivity to Na⁺ stress, decreased growth level at neutral-alkaline pH, and repressed hyphal growth on PDA at 25°C. Truncation of the coding sequence for the conserved signaling protease box of WdPacCp impaired growth and reduced RNA expression of class II chitin synthase gene WdCHS1 at acidic pH, and activated hyphal growth on PDA. My results suggested that WdStuAp and WdPacCp play important roles in yeast-hyphal transitions in W. dermatitidis, and that WdPacCp is particularly important for W. dermatitidis to adapt to different ambient pH conditions.
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Molecular cloning and characterization of SNF1 related protein kinasesin tomato (lycopersicon esculentum)Lam, Pui-Yi., 林佩儀. January 2003 (has links)
published_or_final_version / abstract / toc / Botany / Master / Master of Philosophy
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Molecular cloning and characterization of a novel germ cell-specific gene, VAD1.3, in spermatogenesisShum, Ka-yee, Cathy., 沈嘉儀. January 2003 (has links)
published_or_final_version / abstract / toc / Surgery / Master / Master of Philosophy
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Molecular cloning and functional characterization of goldfish Alpha-2 adrenergic receptorsChan, Hoi-yan, 陳凱恩 January 2004 (has links)
published_or_final_version / abstract / toc / Zoology / Master / Master of Philosophy
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cDNA cloning and characterization of the rat globin genes胡嘉雯, Woo, Carmen. January 1989 (has links)
published_or_final_version / Biochemistry / Master / Master of Philosophy
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