In estuarine and other aquatic systems, it is possible for water to transport locally produced carbon (food) across habitat boundaries, and provide nutrition for animals remote from the carbon source. In estuarine and marine systems, early work examining the movement of carbon from saltmarsh habitats in the USA suggested that carbon may move large distances from inshore to offshore environments. Upon closer examination, however, evidence did not support this paradigm of large-scale carbon movement, referred to as the outwelling hypothesis, in some estuaries. Physical characteristics of estuaries in which large-scale carbon movement did not occur, such as restricted access to the sea, were proposed as a possible explanation, and for these estuaries, movement of carbon among estuarine habitats was considered more likely. A mosaic of saltmarsh and mangrove habitats dominate the subtropical barrier estuary of southern Moreton Bay, Queensland, but there have been no studies that examine the movement of carbon among habitats within this system. Previous studies that examine the movement of carbon have mostly been done in saltmarshes in the northern hemisphere or in tropical mangrove systems. Different vegetation and tidal regimes in temperate marshes of the northern hemisphere preclude generalisations of carbon movement to tropical and subtropical systems. Our understanding of carbon movement in tropical systems may extend to subtropical waters, but the saltmarsh-mangrove mosaic in the subtropics distinguishes them from their tropical counterparts. The mosaic of saltmarsh and mangrove habitats among the barrier islands of southern Moreton Bay thus provide a unique opportunity to examine the small-scale movement of carbon among adjacent habitats in a subtropical system. Stable isotopes of carbon have been used successfully to trace the transfer of carbon from autotrophs to consumers at a range of spatial scales. This method is able to distinguish among carbon sources where autotrophs have different ratios of 13C/12C, and consumers take on the ratio of their food source. The success of stable isotopes in clarifying food web processes, however, depends on isotope ratios changing in predictable ways as elements are processed. As isotope ratios may be influenced by changes in productivity, and differences in nutrient source, they may vary across small and large spatial scales that may confound interpretation of food web processes. In this study I measured small and large-scale spatial variability of three estuarine autotrophs (the saltmarsh grass, Sporobolus virginicus, the seagrass Zostera capricorni and the algal community epiphytic on Z. capricorni) and showed the small-scale spatial variability to be negligible and insufficient to preclude the use of carbon and nitrogen isotopes in food web studies. Large-scale variability was more pronounced and may be useful for spatial correlation of food webs for more mobile species. The small-scale homogeneity and clearly distinguished isotope ratios of the dominant autotrophs in adjacent saltmarsh and mangrove habitats in southeast Queensland are therefore ideally suited to the study of small-scale carbon movement between adjacent habitats. Carbon isotopes of estuarine invertebrates were used to estimate the movement of particulate carbon between adjacent saltmarsh and mangroves at the tens-of-metre scale. Carbon isotope values of two crab species (Parasesarma erythrodactyla and Australoplax tridentata) and two snail species (Salinator solida and Ophicardelus quoyi) in saltmarsh closely match those of the saltmarsh grass, and suggest that the movement and assimilation of carbon occurs at a scale much smaller than has previously been examined. In mangroves, the results of this study indicate that microphytobenthos with some contribution of mangrove carbon is the most likely food source for P. erythrodactyla and A. tridentata, although contribution of carbon from saltmarsh is also possible. Under this latter scenario, carbon movement in mangroves would be considered to occur at a scale larger than that in saltmarsh habitat. A study that examined the movement and assimilation of carbon by crabs and an estuarine slug (Onchidina australis) at a finer resolution (i.e. metres) supported the original findings and indicated that the movement and assimilation of carbon occurs 5 - 8 m either side of the saltmarsh-mangrove interface. At this small-scale, the movement and subsequent foraging of crabs among habitats, the movement of particulate carbon among habitats, or a combination of crab and particulate carbon movement are three alternative models that provide plausible explanations for the pattern in carbon isotope values of crabs. Crab movement among these habitats was measured using an array of pitfall traps perpendicular to the saltmarsh-mangrove interface. To test for carbon movement, samples of detritus were collected at 2 m intervals across this same interface and the carbon isotopes analysed. For the majority of crabs (up to 90% for both species), movement up or down the shore was less than 1 m from the place of initial capture. Thus, crab movement cannot explain the trend in carbon isotope values of crabs. The pattern in detrital isotope values was similar to that of crabs and indicates that the movement of particulate carbon across the saltmarsh-mangrove interface is the most likely explanation for crab isotope ratios. Sources of carbon for estuarine invertebrates can also depend on the size of the saltmarsh patches. Examination of the movement and assimilation of carbon by crabs in saltmarsh patches of different sizes adjacent to mangroves indicates that saltmarshes less than 0.3 ha in area are subsidised by the import of allochthonous carbon, most likely from mangroves. These findings contribute substantially to our understanding of the food web value of estuarine habitats and provide an important link between landscape and food web ecology. They also have important implications for determining the conservation value of estuarine habitats with respect to their functional (food web) value. The scale-dependent sampling used in this thesis also provides important evidence for the fine-scale movement of estuarine carbon that has not previously been examined.

The increasing world population and with it the increased pressure on food production are likely to challenge the availability of quality fresh water resources in the near future. To compound the looming water crisis, caused by an increased demand for water available for agricultural production, the quality of our fresh water resources is also likely to suffer from the consequences of increased population pressure, i.e. urbanization of land and growth of industries, and food production, i.e. agricultural use of land. Moreton Bay, South East Queensland, Australia, is listed under the United Nations Convention on Wetlands and is also a declared Marine Park. The Moreton Bay area, however, is already one of the five fastest growing urban areas in the developed world. Prognoses about future population growth and urban and industrial development in the area, have hence given rise to growing concerns about the future water quality in this international environmentally important area. Therefore the aim of the current study was to investigate the fate of nutrients in freshwater streams in the Moreton Bay area in order to gain a better understanding of nutrient pathways in aquatic systems and assist in refining the National Water Quality Management Strategy to provide better management of our waterways. To achieve this, the effects of land use on water quality were determined at 22 study sites in the Brisbane River Catchment. Within the catchment five main types of land use were identified, including urban, rural residential, cropping, grazing and mixed types of land use. Water quality was sampled during three seasons: the pre-wet (October - November), wet (December - March) and dry (April - August) season. Nitrogen and phosphorus concentrations in ambient stream water varied significantly spatially, i.e. types of land use, and temporally, i.e. seasons. At some sites, during certain times of the year, nutrient concentrations were found to exceed the range recommended by the Australian Water Quality guidelines. Nutrient concentrations were particular high in urban areas, especially during the dry season. It was also found that the 15N signatures in aquatic plants, i.e epipelic algae, correlated strongly with in-stream nitrogen concentrations. The large variability of in-stream nutrient concentrations, and the related changes in nitrogen isotopic signatures in aquatic plants, made it obvious to suggest that changes in land use may significantly impact on water quality in the catchment. Other changes in land use, for example riparian vegetation clearing, are also commonly observed in areas under urban, industry and/or agricultural growth pressure. This is of particular concern, given riparian vegetation is important not only in controlling nutrient and other organic matter input into streams, but also in regulating light levels for in-stream primary production. Previously riparian zones have been shown to be a prime source of carbon and energy for aquatic food webs in some studies, whereas other studies suggested the main driver of food webs is in-stream primary production. The current study used stable isotope analysis track carbon and nitrogen pathways through aquatic systems and determine the primary source of carbon and energy in aquatic food webs. Despite large spatial and temporal variability of 13C, aquatic consumers were closely tracking the carbon isotope signatures of plants and it was suggested that epilithic and epipelic algae are the main contributors to the carbon and energy budget of aquatic consumers.In realizing this importance of algae in aquatic systems, the next step in this study was to examine the relative importance of light and nutrient availability to periphyton and the effects of changes of these variables on plant biomass and primary production. In an in-situ experiment the levels of light and nutrients available to periphyton, were altered. Although nutrients and light may have colimited standing crop of periphyton, other variables were clearly limited by light. Parallel to this experiment on periphyton, the nutrient availability to Vallisneria spp. was experimentally altered to investigate the effects of changes in nutrient availability and nutrient limitation on other aquatic plants. The biomass of this submerged macrophyte increased three-fold in nitrogen and phosphorus sufficient areas over nutrient limited treatments. The physiological response, i.e. changes in concentrations of amino acids, of periphyton to changes in environmental conditions was also investigated on a large scale, i.e. spatial and temporal variability of amino acids, and a local scale, i.e. amino acid changes in artificially altered light and nutrient availability. This response was of particular interest in this study, as it was previously shown that physiological changes in plants impact on the quality of plants as food for consumers. The physiological changes in aquatic plants could thus provide an important link between nutrient input into streams (e.g. from terrestrial sources), impacts on aquatic plants (e.g.. nutrient uptake and physiological responses in plants) and effects on aquatic consumers (e.g. changes in food quality of plants and therefore impacts on biomass, growth and overall health of aquatic consumers).