The Malaysian state of Sabah, in northern Borneo, started massive monoculture forest plantations of fast-growing introduced tree species in the mid-1970's to replace part of the harvested tropical rain forest. Many people, particularly conservationists in the West, are very much against this sort of reforestation as they fear it would spell a permanent loss to the Bornean rain forest biodiversity. This project was carried out at the more established forest plantations of Sabah Softwoods Sdn. Bhd. in Brumas from 1991 to 1993, where fast-growing exotics namely Acacia mangium, Eucalyptus deglupta, Gmelina arborea, Paraserianthes (=Albizia) falcataria, Pinus caribaea, were studied to assess their biodiversity and these plantations were compared with the natural regenerating logged-over secondary forest in Brumas, as well as the primary forest in Danum Valley, by using light-trapped macromoths as indicators. The method of light-trapping as a reliable means of capturing moths was supported by canopy knockdown in the form of mist-blowing. Results obtained showed that for the year-long (January-December 1991) light-trap samples, the biodiversity values, as represented by Williams alpha (higher the value, higher the diversity), were unexpectedly high in the various plantation forests. Their alpha values ranged from the lowest in Acacia mangium with 208.14+-9.22, to the highest in Eucalyptus deglupta with 330.85+-16.37 which was even higher than the natural secondary forest with 314.53+-11.99, and certainly not inferior to the published values (300 to 350) from undisturbed Bornean forest of similar altitudes (below 500m). For the shorter month-long subsidiary samples (October/November 1992, January/February 1993), the alpha values of the samples from the lowland primary forest in Danum were not necessarily higher when compared with the similarly sampled disturbed forest habitats in Brumas, but despite its small samples, Danum produced some 33 species of macromoths which were never collected out of the 1680 species obtained from Brumas in the entire project. The main reason behind the surprisingly good diversity measures (as indicated by moths) in these forest plantations was the presence of an understorey of varying diversity under the canopy. It would appear that with the fast-growing introduced trees acting as light-demanding pioneers, many plant species ranging from herbs, shrubs, to saplings of native tree species, managed to germinate and grow more or less efficiently in the understorey. Eucalyptus deglupta had a more diverse understorey both in terms of plant species and architecture, which in turn supported a more diverse moth fauna. These findings are encouraging in terms of biodiversity conservation, as plantation forestry seems to be the only way forward for many developing countries like Malaysia.

Hemolymph trehalose concentrations of male and female Heliothis zea were quantitated by high performance thin-layer chromatography (HPTLC) at various ages and times of the day and related to flight activity. Effects of octopamine injection or stress on trehalose levels were also quantified. Flight activity was measured with a 32-channel computerized actograph that simulated sunrise at 0300 EST (Eastern Standard Time) and sunset at 1700. Males exhibited greater flight activity than females at all ages examined. Flight began near sunset, continuing through the night and ending around sunrise. Females flew continuously throughout the night, whereas males exhibited two peaks in activity: the first between 1700 and 1900, followed by a second peak between 2000 and 2400. Flight activity peaked on days 3-4 in males and days 4- 5 in females. Trehalose was the predominant hemolymph sugar, comprising 82-100% of total hemolymph sugars. Glucose was the second most frequently observed sugar. Trehalose concentrations were variable, ranging from < 1 ug/ul to 37 ug/ul. Lab-reared moths had higher and more variable trehalose concentrations than field collected (wild) moths. Trehalose levels were relatively constant several days after emergence in both sexes but decreased significantly by day 6 in males. When examined over a 24 hour period, trehalose concentrations gradually increased throughout the day in day 4 males and females, peaking one hour before sunset at approximately 18 ug/ul. This peak is hypothesized to be related to flight preparation. Females showed a second peak in trehalose levels at 1800, but males’ trehalose levels continued to decline until 1900. Males and females exhibited more similar patterns in trehalose concentrations when sampled every 15 minutes over the sunset period (1500-1900) than when sampled at hour intervals. Injections of octopamine, reputed to induce hyperglycemia in other insects, failed to elicit significant increases in trehalose levels in either fed or starved moths. Starved moths had lower trehalose concentrations than fed moths. Method of analysis (HPLC, HPTLC and anthrone) produced no differences in measured levels of trehalose or glucose. Various forms of stress (handling, shaking), also reported to induce hyperglycemia in other insects, similarly did not significantly increase trehalose titers in moths. / Ph. D.