In this thesis, we proposed the series methods of moving object segmentation and object application. These methods are the moving object segmentation method in wavelet domain, double change detection method, global motion estimation method, and the moving object segmentation in the motion background. First, we proposed the Video Object Segmentation Method in Wavelet Domain. We use the Change Detection Method with the different thresholds in four wavelet sub-bands. The experiment results show that we obtain further object shape information and more accurately extracting the moving object. In the double change detection method, we proposed the method for moving object segmentation using three successive frames. We use change detection method twice in wavelet domain. After applying the Intersect Operation, we obtain the accurately moving object edge map and further object shape information. Besides, we proposed the global motion estimation method in motion scene. We propose a novel global motion estimation using cross point for the reconstruction of background scene in video sequences. Due to the robust character and limit number of cross points, we can get the Affine parameters of global motion in video sequences efficiency. At last, we proposed the object segmentation method in motion scene. We use the motion estimation method to estimate the global motion between the consecutive frames. We reconstruct a wide scene background without moving objects by the consecutive frames. At last, the moving objects will be segmented easily by comparing the object frame and the relative part in wide scene background. The Results of our proposed have good performance in the different type of video sequences. Hence, the methods of our thesis contribute to the video coding in Mpeg-4 and multimedia technology.