Doctor Educationis / Cybersecurity defense tools, techniques and methodologies are constantly faced with increasing challenges including the evolution of highly intelligent and powerful new generation threats. The main challenges posed by these modern digital multi-vector attacks is their ability to adapt with machine learning. Research shows that many existing defense systems fail to provide adequate protection against these latest threats. Hence, there is an ever-growing need for self-learning technologies that can autonomously adjust according to the behaviour and patterns of the offensive actors and systems. The accuracy and effectiveness of existing methods are dependent on decision making and manual input by human expert. This dependence causes 1) administration overhead, 2) variable and potentially limited accuracy and 3) delayed response time. In this thesis, Autonomous Threat Detection and Response (ATDR) is a proposed general method aimed at contributing toward security related self-protected networks. Through a combination of unsupervised machine learning and Deep learning, ATDR is designed as an intelligent and autonomous decision-making system that uses big data processing requirements and data frame pattern identification layers to learn sequences of patterns and derive real-time data formations. This system enhances threat detection and response capabilities, accuracy and speed. Research provided a solid foundation for the proposed method around the scope of existing methods and the unanimous problem statements and findings by other authors.